Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner
Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.
FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.
Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.
Human Cells Grow With or Without Replacement for Fetal Bovine Serum
security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.
Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.
The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).
Czapek Dox Liquid Medium |
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M1170A-500G | EWC Diagnostics | 1 unit | EUR 40.36 |
Description: Czapek Dox Liquid Medium |
Sabouraud Liquid Medium - EACH |
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MED1462 | Scientific Laboratory Supplies | EACH | EUR 71.55 |
MS LIQUID MEDIUM w/ SUGARS |
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M5615 | PhytoTechnology Laboratories | 1000ML | EUR 14.81 |
FRIIS BASE Medium, Liquid |
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F2485-100 | GenDepot | 100ml | Ask for price |
FRIIS BASE Medium, Liquid |
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F2485-500 | GenDepot | 500ml | Ask for price |
Liquid Wicks, Size Medium, 200/pk |
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W-M | MiTeGen | 200 WICKS | EUR 22 |
Description: Liquid Wicks, Size Medium, 200/pk |
LIQUID AMIES VIRAL TRANPORT MEDIUM |
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7574003 | Lampire Biologicals Laboratories | 1ML | EUR 15.85 |
Exosome-depleted FBS Media Supplement |
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EXO-FBS-250A-1 | SBI | 250 ml | EUR 837 |
Exosome-depleted FBS Media Supplement |
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EXO-FBS-50A-1 | SBI | 50 ml | EUR 193 |
Viral Transport Medium Liquid Amies |
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IGVTMA1000ML | Innovative research | each | EUR 145 |
Description: Viral Transport Medium Liquid Amies |
Viral Transport Medium Liquid Amies |
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IGVTMA500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Liquid Amies |
Tryptic Soy Broth (TSB) Medium, Liquid |
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TBS8065-1L | Tribioscience | 1L | EUR 35 |
Air Liquid Interface Differentiation Medium |
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TM091 | ABM | 500 ml | EUR 540 |
LIQUID AMIES VIRAL TRANPORT MEDIUM, 1 ML |
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7574003-A | Lampire Biologicals Laboratories | 1ML | EUR 6.33 |
LIQUID AMIES VIRAL TRANPORT MEDIUM, 1 LTR |
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7574003-1LTR | Lampire Biologicals Laboratories | 1LTR | EUR 23.17 |
C1q Liquid |
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pro-554L | ProSpec Tany | 200µg | EUR 295 |
Description: Human Complement Component C1q |
Paraffin Liquid |
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26114-04 | NACALAI TESQUE | 15KG | EUR 157.5 |
Paraffin Liquid |
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26114-75 | NACALAI TESQUE | 500ML | EUR 9.59 |
Paraffin Liquid |
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26117-45 | NACALAI TESQUE | 500ML | EUR 13.3 |
Paraffin Liquid |
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26133-54 | NACALAI TESQUE | 100ML | EUR 24.85 |
Paraffin Liquid |
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26137-85 | NACALAI TESQUE | 500ML | EUR 26.25 |
Formamide (Liquid) |
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MB012-1L | EWC Diagnostics | 1 unit | EUR 39.73 |
Description: Formamide (Liquid) |
Formamide (Liquid) |
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MB012-500ML | EWC Diagnostics | 1 unit | EUR 26.43 |
Description: Formamide (Liquid) |
Dimethylmalonate (Liquid) |
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RM2789-500G | EWC Diagnostics | 1 unit | EUR 108.95 |
Description: Dimethylmalonate (Liquid) |
Vitamin E, liquid |
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GK3575-100 | Glentham Life Sciences | 100 | EUR 123.4 |
Liquid Wicks assortment, 15, XF, F,and Medium, 200 of each size |
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W-A1 | MiTeGen | 800 WICKS | EUR 66 |
Description: Liquid Wicks assortment, 15, XF, F,and Medium, 200 of each size |
Hayflick Medium Complete with ampicillin and Thallium, Liquid |
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H2486-100 | GenDepot | 100ml | Ask for price |
D.P.X. mountant (Liquid) |
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GRM655-500G | EWC Diagnostics | 1 unit | EUR 14.77 |
Description: D.P.X. mountant (Liquid) |
Soya lecithin liquid |
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GRM6398-100ML | EWC Diagnostics | 1 unit | EUR 8.76 |
Description: Soya lecithin liquid |
Soya lecithin liquid |
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GRM6398-500ML | EWC Diagnostics | 1 unit | EUR 39.16 |
Description: Soya lecithin liquid |
ABTS Liquid Substrate |
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21530043-1 | Glycomatrix | 100 mL | EUR 57.34 |
Paraffin Liquid, light |
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26132-35 | NACALAI TESQUE | 500ML | EUR 21.7 |
Paraffin Liquid, light |
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26132-64 | NACALAI TESQUE | 16KG | EUR 238 |
Paraffin Liquid, light |
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26144-85 | NACALAI TESQUE | 500ML | EUR 57.4 |
PNPP Liquid Substrate |
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F008-100 | Cygnus Technologies | 100 ml | EUR 280.8 |
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur. |
PNPP Liquid Substrate |
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F008-1000 | Cygnus Technologies | 1000 ml | EUR 890.4 |
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur. |
Paraffin liquid, heavy |
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GRM6362-2.5L | EWC Diagnostics | 1 unit | EUR 25.94 |
Description: Paraffin liquid, heavy |
Paraffin liquid, heavy |
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GRM6362-500ML | EWC Diagnostics | 1 unit | EUR 6.93 |
Description: Paraffin liquid, heavy |
Paraffin liquid, light |
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GRM1310-2.5L | EWC Diagnostics | 1 unit | EUR 18.12 |
Description: Paraffin liquid, light |
Paraffin liquid, light |
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GRM1310-500ML | EWC Diagnostics | 1 unit | EUR 6.93 |
Description: Paraffin liquid, light |
(±)-a-Tocopherol, liquid |
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CMS185-10G | EWC Diagnostics | 1 unit | EUR 9.07 |
Description: (±)-a-Tocopherol, liquid |
alpha-Amylase, liquid |
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GK2343-25 | Glentham Life Sciences | 25 | EUR 47.4 |
alpha-Amylase, liquid |
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GK2343-25ML | Glentham Life Sciences | 25 ml | EUR 122.4 |
LD4 Liquid Dewar Cap |
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TW-D004-9C11 | MiTeGen | 1 UNIT | EUR 23 |
Description: LD4 Liquid Dewar Cap |
BenzAlt? Binuclease (Liquid) |
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9215-10 | Biovision | each | EUR 202.8 |
BenzAlt? Binuclease (Liquid) |
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9215-50 | Biovision | each | EUR 444 |
Sunflower lecithin, liquid |
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GE1230-1 | Glentham Life Sciences | 1 | EUR 144 |
Sunflower lecithin, liquid |
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GE1230-100 | Glentham Life Sciences | 100 | EUR 27.8 |
Sunflower lecithin, liquid |
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GE1230-100G | Glentham Life Sciences | 100 g | EUR 69.6 |
Sunflower lecithin, liquid |
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GE1230-1KG | Glentham Life Sciences | 1 kg | EUR 210 |
Sunflower lecithin, liquid |
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GE1230-500 | Glentham Life Sciences | 500 | EUR 82.3 |
Sunflower lecithin, liquid |
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GE1230-500G | Glentham Life Sciences | 500 g | EUR 135.6 |
2,6-Dimethylaniline (Liquid) |
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RM2786-100G | EWC Diagnostics | 1 unit | EUR 37.27 |
Description: 2,6-Dimethylaniline (Liquid) |
2,4-Dimethylaniline (Liquid) |
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RM4214-100G | EWC Diagnostics | 1 unit | EUR 16.27 |
Description: 2,4-Dimethylaniline (Liquid) |
Protein A (Liquid form) |
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6500-10 | Biovision | each | EUR 183.6 |
Protein A (Liquid form) |
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6500-100 | Biovision | each | EUR 874.8 |
Protein A (Liquid form) |
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6500-1000 | Biovision | each | EUR 2245.2 |
Protein A (Liquid form) |
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6500-25 | Biovision | each | EUR 314.4 |
Protein A (Liquid form) |
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P1088-10 | ApexBio | 10 mg | EUR 139 |
Description: Protein A, used for purification of IgG antibodies, expressed in E.Coli. |
Protein A (Liquid form) |
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P1088-100 | ApexBio | 100 mg | EUR 1052.4 |
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. |
Protein A (Liquid form) |
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P1088-1000 | ApexBio | 1 g | EUR 2722.8 |
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. |
Protein A (Liquid form) |
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P1088-25 | ApexBio | 25 mg | EUR 369.6 |
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. |
EPO alpha, Liquid, human |
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RC213-15L | Bio Basic | 500IU | EUR 125.26 |
TBE 10X Liquid Solution |
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20-196 | Genesee Scientific | 4 Liters/Unit | EUR 92.41 |
Description: Ultra Pure Grade Solution |
DAB Two component liquid |
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AR-8206-01 | ImmunoBioscience | 100 ml | EUR 61.4 |
DAB Two component liquid |
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AR-8206-02 | ImmunoBioscience | 200 ml | EUR 104.3 |
DAB Two component liquid |
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AR-8206-05 | ImmunoBioscience | 1000 ml | EUR 331.8 |
1 L SSPE, 20X, LIQUID |
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46-021-CM | CORNING | 1L/pk | EUR 242.4 |
Description: Media Catalog; Classical Media |
Recombinant Streptavidin, Liquid |
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AOOPP-0501 | Cyagen | 10mg | EUR 2207.45 |
TAE 50X Liquid Concentrate |
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20-193 | Genesee Scientific | 1.6 Liters/Unit | EUR 156.79 |
Description: Ultra Pure Grade Solution |
TAE 50X Liquid Concentrate |
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20-194 | Genesee Scientific | 4 Liters/Unit | EUR 228.33 |
Description: Ultra Pure Grade Solution |
TAE 25X Liquid Concentrate |
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20-195 | Genesee Scientific | 1.6 Liters/Unit | EUR 90.13 |
Description: Ultra Pure Grade Solution |
DAB Liquid Concentrate 50X |
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21530109-1 | Glycomatrix | 10 mL | EUR 92.44 |
DAB Liquid Concentrate 50X |
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21530109-2 | Glycomatrix | 25 mL | EUR 137.75 |
DAB Liquid Concentrate 50X |
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21530109-3 | Glycomatrix | 100 mL | EUR 388.67 |
DAB Liquid Concentrate 10X |
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21530113-1 | Glycomatrix | 100 mL | EUR 99.4 |
DAB Liquid Concentrate 10X |
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21530113-2 | Glycomatrix | 500 mL | EUR 259.7 |
DMEM-Glucose Free, liquid |
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TBS8061GF-1L | Tribioscience | 1L | EUR 39 |
DMEM-Glucose Free, Liquid |
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TBS8061GF-500mL | Tribioscience | 500mL | EUR 28 |
Mucasol Liquid Detergent 2L |
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HAE3204 | Scientific Laboratory Supplies | EACH | EUR 166.44 |
Desderman pure Liquid 500 mL - PK20 |
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CLE3234 | Scientific Laboratory Supplies | PK20 | EUR 380.42 |
Gamborg's Vitamin Stcks, Liquid |
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CP049-010 | GenDepot | 125 ml bottle | EUR 128.4 |
100mL Cellstripper Liquid - PK6 |
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25-056-CI | Scientific Laboratory Supplies | PK6 | EUR 130.95 |
Hanna GP Liquid Probe - EACH |
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THE4525 | Scientific Laboratory Supplies | EACH | EUR 133.65 |
LD4 Liquid Dewar (4 liters) |
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TW-LD4 | MiTeGen | 4 liter | EUR 808 |
Description: LD4 Liquid Dewar (4 liters) |
LD5 Liquid Dewar (5 liters) |
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TW-LD5 | MiTeGen | 5 liter | EUR 1049 |
Description: LD5 Liquid Dewar (5 liters) |
IceOff Liquid Nitrogen Drizzler |
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API-IceOff-01 | MiTeGen | each | EUR 324 |
Description: IceOff Liquid Nitrogen Drizzler |
IceOff Liquid Nitrogen Drizzler |
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TE | MiTeGen | 1 UNIT | EUR 324 |
Description: IceOff Liquid Nitrogen DrizzlereNTER TESTNO NIE |
LD10 Liquid Dewar (10 liters) |
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TW-LD10 | MiTeGen | 10 liter | EUR 748 |
Description: LD10 Liquid Dewar (10 liters) |
LD25 Liquid Dewar (25 liters) |
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TW-LD25 | MiTeGen | 25 liter | EUR 1155 |
Description: LD25 Liquid Dewar (25 liters) |
LD35 Liquid Dewar (35 liters) |
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TW-LD35 | MiTeGen | 35 liter | EUR 1196 |
Description: LD35 Liquid Dewar (35 liters) |
LD50 Liquid Dewar (50 Liters) |
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TW-LD50 | MiTeGen | 50 liter | EUR 1612 |
Description: LD50 Liquid Dewar (50 Liters) |
Automatic Liquid Nitrogen Filler |
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M-CEM-37454 | MiTeGen | 1 UNIT | EUR 3850 |
Description: Automatic Liquid Nitrogen Filler |
1 L TE BUFFER, 1X, LIQUID |
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46-009-CM | CORNING | 1L/pk | EUR 157.2 |
Description: Media Catalog; Cell Culture Reagents |
TG-SDS 10X Liquid Solution |
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20-247 | Genesee Scientific | 4 Liters/Unit | EUR 97.29 |
Description: Ultra Pure Grade |
Liquid Fuel Burner 100mL - EACH |
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BUR4502 | Scientific Laboratory Supplies | EACH | EUR 8.1 |
Cryo Kit for liquid nitrogen |
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IPD9600-CK | Benchmark Scientific | each | EUR 1027.94 |
Liquid Master 600mm - PTFE - EACH |
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SAM1106 | Scientific Laboratory Supplies | EACH | EUR 357.75 |
Liquid Master 1000mm - PTFE - EACH |
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SAM1107 | Scientific Laboratory Supplies | EACH | EUR 460.35 |
Liquid Master 1500mm - PTFE - EACH |
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SAM1108 | Scientific Laboratory Supplies | EACH | EUR 571.05 |
Liquid Master 2000mm - PTFE - EACH |
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SAM1109 | Scientific Laboratory Supplies | EACH | EUR 648 |
Liquid Master 2500mm - PTFE - EACH |
|||
SAM1110 | Scientific Laboratory Supplies | EACH | EUR 819.45 |
Recombinant Rat IL-2, Liquid |
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REILP-0202 | Cyagen | 5ug | Ask for price |
Swab Liquid MRD Viscose - PK100 |
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SWA1124 | Scientific Laboratory Supplies | PK100 | EUR 141.75 |
Liquid Wicks, Size 15, 200 /pk |
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W-15 | MiTeGen | 200 WICKS | EUR 22 |
Description: Liquid Wicks, Size 15, 200 /pk |
100mL glutagro Liquid 200 mM Sol - EACH |
|||
25-015-CI | Scientific Laboratory Supplies | EACH | EUR 55.35 |
TMB Liquid Substrate, for ELISA |
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F027-100 | Cygnus Technologies | 100 ml | EUR 262.8 |
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur. |
TMB Liquid Substrate, for ELISA |
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F100027 | Cygnus Technologies | 1000 ml | EUR 817.2 |
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur. |
Aquet liquid detergent 3.8l - EACH |
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Z273260-1EA | Scientific Laboratory Supplies | EACH | EUR 109.35 |
RPMI 1640 with L-Gln, liquid |
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30264-56 | NACALAI TESQUE | 10X500ML | EUR 80.5 |
RPMI 1640 with L-Gln, liquid |
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30264-85 | NACALAI TESQUE | 500ML | EUR 9.8 |
1 L TBE BUFFER, 10X, LIQUID |
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46-011-CM | CORNING | 1L/pk | EUR 141.6 |
Description: Media Catalog; Classical Media |
Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified |
|||
EXO-FBSHI-250A-1 | SBI | 250 ml | EUR 850 |
Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified |
|||
EXO-FBSHI-50A-1 | SBI | 50 ml | EUR 206 |
Liquid Wicks, Size Fine, 200/pk |
|||
W-F | MiTeGen | 200 WICKS | EUR 22 |
Description: Liquid Wicks, Size Fine, 200/pk |
TE Buffer 10X, Liquid Concentrate |
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10530029-1 | Glycomatrix | 100 mL | EUR 18.02 |
TE Buffer 10X, Liquid Concentrate |
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10530029-2 | Glycomatrix | 500 mL | EUR 43 |
TE Buffer 10X, Liquid Concentrate |
|||
10530029-3 | Glycomatrix | 250 mL | EUR 27.17 |
TE Buffer 10X, Liquid Concentrate |
|||
10530029-4 | Glycomatrix | 1 L | EUR 93.89 |
Influenza A (H1N1) Antigen Liquid |
|||
IFLUAH1N1ALR1ML | Innovative research | each | EUR 771 |
Description: Influenza A (H1N1) Antigen Liquid |
Influenza A (H3N2) Antigen Liquid |
|||
IFLUAH3N2ALR1ML | Innovative research | each | EUR 754 |
Description: Influenza A (H3N2) Antigen Liquid |
Mucasol Liquid Detergent 5L - EACH |
|||
HAE3206 | Scientific Laboratory Supplies | EACH | EUR 391.5 |
G418 SULPHATE (LIQUID, 50 MG/ML) |
|||
30-234-CR | CORNING | 20 mL/pk | EUR 116.4 |
Description: Media Catalog; Cell Culture Reagents |
Liquid Wicks, Extra Fine, 200/pk |
|||
W-XF | MiTeGen | 200 WICKS | EUR 22 |
Description: Liquid Wicks, Extra Fine, 200/pk |
TAE Buffer 10X, Liquid Concentrate |
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10530012-1 | Glycomatrix | 1 L | EUR 51.77 |
TAE Buffer 10X, Liquid Concentrate |
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10530012-2 | Glycomatrix | 4 L | EUR 157.04 |
TAE Buffer 25X, Liquid Concentrate |
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10530013-1 | Glycomatrix | 1 L | EUR 44.84 |
TAE Buffer 25X, Liquid Concentrate |
|||
10530013-2 | Glycomatrix | 4 L | EUR 127.23 |
TAE Buffer 50X, Liquid Concentrate |
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10530016-1 | Glycomatrix | 500 mL | EUR 43 |
TAE Buffer 50X, Liquid Concentrate |
|||
10530016-2 | Glycomatrix | 1 L | EUR 69.32 |
TAE Buffer 50X, Liquid Concentrate |
|||
10530016-3 | Glycomatrix | 4 L | EUR 170.21 |
TBE Buffer Liquid Concentrate 10X |
|||
10530017-1 | Glycomatrix | 1 L | EUR 43.83 |
TBE Buffer Liquid Concentrate 10X |
|||
10530017-2 | Glycomatrix | 4 L | EUR 120.2 |
TBE Buffer Liquid Concentrate 10X |
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10530017-3 | Glycomatrix | 500 mL | EUR 28.07 |
TBE Buffer Liquid Concentrate 25X |
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10530022-1 | Glycomatrix | 1 L | EUR 65.31 |
TBE Buffer Liquid Concentrate 25X |
|||
10530022-2 | Glycomatrix | 4 L | EUR 224.93 |
SSC Buffer Liquid Concentrate 20X |
|||
10750018-1 | Glycomatrix | 1 L | EUR 59.66 |
SSC Buffer Liquid Concentrate 20X |
|||
10750018-2 | Glycomatrix | 4 L | EUR 112 |
SSC Buffer Liquid Concentrate 20X |
|||
10750018-3 | Glycomatrix | 20 L | EUR 349.19 |
TBS Buffer Liquid Concentrate 20X |
|||
42020064-1 | Glycomatrix | 4 L | EUR 102.65 |
TBS Buffer Liquid Concentrate 20X |
|||
42020064-2 | Glycomatrix | 1 L | EUR 29.83 |
TBS Buffer Liquid Concentrate 20X |
|||
42020064-3 | Glycomatrix | 6 L | EUR 146.75 |
SSC Buffer - 20x Liquid Concentrate |
|||
D0623 | Bio Basic | 1L | EUR 74.62 |
RPMI 1640 without L-Gln, liquid |
|||
05176-25 | NACALAI TESQUE | 500ML | EUR 10.5 |
1L TSB animal free Liquid - EACH |
|||
46-060-CM | Scientific Laboratory Supplies | EACH | EUR 199.8 |
100mL-Glutamine 200 mM Liquid - PK6 |
|||
25-005-CI | Scientific Laboratory Supplies | PK6 | EUR 133.65 |
SSPE Buffer Liquid Concentrate 20X |
|||
10750024-1 | Glycomatrix | 1 L | EUR 49.13 |
SSPE Buffer Liquid Concentrate 20X |
|||
10750024-2 | Glycomatrix | 4 L | EUR 165.83 |
Tris-Glycine 10X Liquid Solution |
|||
20-246 | Genesee Scientific | 4 Liters/Unit | EUR 120.21 |
Description: Ultra Pure Grade |
Paraffin Liquid, Low Viscosity Type |
|||
25990-25 | NACALAI TESQUE | 500ML | EUR 18.55 |
Label 100X100 Flammable Liquid.. - EACH |
|||
C35AR | Scientific Laboratory Supplies | EACH | EUR 121.5 |
20L TSB animal free Liquid - EACH |
|||
46-060-LB | Scientific Laboratory Supplies | EACH | EUR 1613.25 |
Paraffin Liquid, High Viscosity Type |
|||
25991-15 | NACALAI TESQUE | 500ML | EUR 12.95 |
LN20 Liquid Nitrogen Generator - EACH |
|||
SLS6150 | Scientific Laboratory Supplies | EACH | EUR 53978.4 |
LN30 Liquid Nitrogen Generator - EACH |
|||
SLS6154 | Scientific Laboratory Supplies | EACH | EUR 69400.8 |
LN65 Liquid Nitrogen Generator - EACH |
|||
SLS6158 | Scientific Laboratory Supplies | EACH | EUR 88678.8 |
Paraffin liquid, For IR Spectroscopy |
|||
GRM1136-100ML | EWC Diagnostics | 1 unit | EUR 6.93 |
Description: Paraffin liquid, For IR Spectroscopy |
Paraffin liquid, For IR Spectroscopy |
|||
GRM1136-500ML | EWC Diagnostics | 1 unit | EUR 20.07 |
Description: Paraffin liquid, For IR Spectroscopy |
Ham's F-12 with L-Gln, liquid |
|||
17458-65 | NACALAI TESQUE | 500ML | EUR 9.8 |
Human ESD Recombinant Protein Liquid |
|||
IHUESDESDR100UG | Innovative research | each | EUR 721 |
Description: Human ESD Recombinant Protein Liquid |
Human KHK Recombinant Protein Liquid |
|||
IHUKHKKHKR50UG | Innovative research | each | EUR 721 |
Description: Human KHK Recombinant Protein Liquid |
Human PEF Recombinant Protein Liquid |
|||
IHUPEFPEFR50UG | Innovative research | each | EUR 899 |
Description: Human PEF Recombinant Protein Liquid |
Human PXF Recombinant Protein Liquid |
|||
IHUPXFPXFR100UG | Innovative research | each | EUR 899 |
Description: Human PXF Recombinant Protein Liquid |
Human p53 Recombinant Protein Liquid |
|||
IHUP53R25UG | Innovative research | each | EUR 681 |
Description: Human p53 Recombinant Protein Liquid |
Human p62 Recombinant Protein Liquid |
|||
IHUP62R25UG | Innovative research | each | EUR 681 |
Description: Human p62 Recombinant Protein Liquid |
Human LXN Recombinant Protein Liquid |
|||
IHULXNLXNR100UG | Innovative research | each | EUR 1110 |
Description: Human LXN Recombinant Protein Liquid |
Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
Keywords: C2BBe1; SH-SY5Y; fetal bovine serum; neurospheres; xeno-free.