Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.

FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.

Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner
Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Human Cells Grow With or Without Replacement for Fetal Bovine Serum

security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.

Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.

The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).

Czapek Dox Liquid Medium

M1170A-500G 1 unit
EUR 40.36
Description: Czapek Dox Liquid Medium

Sabouraud Liquid Medium - EACH

MED1462 EACH
EUR 71.55

MS LIQUID MEDIUM w/ SUGARS

M5615 1000ML
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FRIIS BASE Medium, Liquid

F2485-100 100ml Ask for price

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LIQUID AMIES VIRAL TRANPORT MEDIUM

7574003 1ML
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Exosome-depleted FBS Media Supplement

EXO-FBS-250A-1 250 ml
EUR 837

Exosome-depleted FBS Media Supplement

EXO-FBS-50A-1 50 ml
EUR 193

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IGVTMA1000ML each
EUR 145
Description: Viral Transport Medium Liquid Amies

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Description: Viral Transport Medium Liquid Amies

Tryptic Soy Broth (TSB) Medium, Liquid

TBS8065-1L 1L
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Air Liquid Interface Differentiation Medium

TM091 500 ml
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7574003-A 1ML
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7574003-1LTR 1LTR
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C1q Liquid

pro-554L 200µg
EUR 295
Description: Human Complement Component C1q

Paraffin Liquid

26114-04 15KG
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26114-75 500ML
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26117-45 500ML
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26133-54 100ML
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Paraffin Liquid

26137-85 500ML
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Formamide (Liquid)

MB012-1L 1 unit
EUR 39.73
Description: Formamide (Liquid)

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Dimethylmalonate (Liquid)

RM2789-500G 1 unit
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GK3575-100 100
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Liquid Wicks assortment, 15, XF, F,and Medium, 200 of each size

W-A1 800 WICKS
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H2486-100 100ml Ask for price

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GRM6398-500ML 1 unit
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21530043-1 100 mL
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Paraffin Liquid, light

26132-35 500ML
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Paraffin Liquid, light

26132-64 16KG
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Paraffin Liquid, light

26144-85 500ML
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PNPP Liquid Substrate

F008-100 100 ml
EUR 280.8
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

PNPP Liquid Substrate

F008-1000 1000 ml
EUR 890.4
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GRM6362-2.5L 1 unit
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GK2343-25 25
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GK2343-25ML 25 ml
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TW-D004-9C11 1 UNIT
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BenzAlt? Binuclease (Liquid)

9215-10 each
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BenzAlt? Binuclease (Liquid)

9215-50 each
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GE1230-1 1
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GE1230-100 100
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GE1230-100G 100 g
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GE1230-1KG 1 kg
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GE1230-500G 500 g
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6500-10 each
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6500-100 each
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Protein A (Liquid form)

6500-1000 each
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Protein A (Liquid form)

6500-25 each
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Protein A (Liquid form)

P1088-10 10 mg
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Description: Protein A, used for purification of IgG antibodies, expressed in E.Coli.

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P1088-100 100 mg
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P1088-1000 1 g
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P1088-25 25 mg
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RC213-15L 500IU
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20-196 4 Liters/Unit
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AR-8206-01 100 ml
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AR-8206-02 200 ml
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AR-8206-05 1000 ml
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46-021-CM 1L/pk
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AOOPP-0501 10mg
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20-193 1.6 Liters/Unit
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20-194 4 Liters/Unit
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20-195 1.6 Liters/Unit
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21530109-1 10 mL
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21530109-2 25 mL
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21530109-3 100 mL
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21530113-1 100 mL
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21530113-2 500 mL
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TBS8061GF-1L 1L
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TBS8061GF-500mL 500mL
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Mucasol Liquid Detergent 2L

HAE3204 EACH
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CLE3234 PK20
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Gamborg's Vitamin Stcks, Liquid

CP049-010 125 ml bottle
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25-056-CI PK6
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THE4525 EACH
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TW-LD4 4 liter
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TW-LD5 5 liter
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IceOff Liquid Nitrogen Drizzler

API-IceOff-01 each
EUR 324
Description: IceOff Liquid Nitrogen Drizzler

IceOff Liquid Nitrogen Drizzler

TE 1 UNIT
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Description: IceOff Liquid Nitrogen DrizzlereNTER TESTNO NIE

LD10 Liquid Dewar (10 liters)

TW-LD10 10 liter
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TW-LD25 25 liter
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M-CEM-37454 1 UNIT
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Description: Automatic Liquid Nitrogen Filler

1 L TE BUFFER, 1X, LIQUID

46-009-CM 1L/pk
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Description: Media Catalog; Cell Culture Reagents

TG-SDS 10X Liquid Solution

20-247 4 Liters/Unit
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Liquid Fuel Burner 100mL - EACH

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IPD9600-CK each
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Recombinant Rat IL-2, Liquid

REILP-0202 5ug Ask for price

Swab Liquid MRD Viscose - PK100

SWA1124 PK100
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W-15 200 WICKS
EUR 22
Description: Liquid Wicks, Size 15, 200 /pk

100mL glutagro Liquid 200 mM Sol - EACH

25-015-CI EACH
EUR 55.35

TMB Liquid Substrate, for ELISA

F027-100 100 ml
EUR 262.8
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.

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F100027 1000 ml
EUR 817.2
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Aquet liquid detergent 3.8l - EACH

Z273260-1EA EACH
EUR 109.35

RPMI 1640 with L-Gln, liquid

30264-56 10X500ML
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RPMI 1640 with L-Gln, liquid

30264-85 500ML
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1 L TBE BUFFER, 10X, LIQUID

46-011-CM 1L/pk
EUR 141.6
Description: Media Catalog; Classical Media

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-250A-1 250 ml
EUR 850

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-50A-1 50 ml
EUR 206

Liquid Wicks, Size Fine, 200/pk

W-F 200 WICKS
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TE Buffer 10X, Liquid Concentrate

10530029-1 100 mL
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TE Buffer 10X, Liquid Concentrate

10530029-2 500 mL
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TE Buffer 10X, Liquid Concentrate

10530029-3 250 mL
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TE Buffer 10X, Liquid Concentrate

10530029-4 1 L
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Influenza A (H1N1) Antigen Liquid

IFLUAH1N1ALR1ML each
EUR 771
Description: Influenza A (H1N1) Antigen Liquid

Influenza A (H3N2) Antigen Liquid

IFLUAH3N2ALR1ML each
EUR 754
Description: Influenza A (H3N2) Antigen Liquid

Mucasol Liquid Detergent 5L - EACH

HAE3206 EACH
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G418 SULPHATE (LIQUID, 50 MG/ML)

30-234-CR 20 mL/pk
EUR 116.4
Description: Media Catalog; Cell Culture Reagents

Liquid Wicks, Extra Fine, 200/pk

W-XF 200 WICKS
EUR 22
Description: Liquid Wicks, Extra Fine, 200/pk

TAE Buffer 10X, Liquid Concentrate

10530012-1 1 L
EUR 51.77

TAE Buffer 10X, Liquid Concentrate

10530012-2 4 L
EUR 157.04

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10530013-1 1 L
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TAE Buffer 25X, Liquid Concentrate

10530013-2 4 L
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TAE Buffer 50X, Liquid Concentrate

10530016-1 500 mL
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TAE Buffer 50X, Liquid Concentrate

10530016-2 1 L
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TAE Buffer 50X, Liquid Concentrate

10530016-3 4 L
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TBE Buffer Liquid Concentrate 10X

10530017-1 1 L
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TBE Buffer Liquid Concentrate 10X

10530017-2 4 L
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TBE Buffer Liquid Concentrate 10X

10530017-3 500 mL
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TBE Buffer Liquid Concentrate 25X

10530022-1 1 L
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TBE Buffer Liquid Concentrate 25X

10530022-2 4 L
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SSC Buffer Liquid Concentrate 20X

10750018-1 1 L
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SSC Buffer Liquid Concentrate 20X

10750018-2 4 L
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10750018-3 20 L
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TBS Buffer Liquid Concentrate 20X

42020064-1 4 L
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42020064-2 1 L
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42020064-3 6 L
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SSC Buffer - 20x Liquid Concentrate

D0623 1L
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RPMI 1640 without L-Gln, liquid

05176-25 500ML
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1L TSB animal free Liquid - EACH

46-060-CM EACH
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100mL-Glutamine 200 mM Liquid - PK6

25-005-CI PK6
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SSPE Buffer Liquid Concentrate 20X

10750024-1 1 L
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SSPE Buffer Liquid Concentrate 20X

10750024-2 4 L
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Tris-Glycine 10X Liquid Solution

20-246 4 Liters/Unit
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Paraffin Liquid, Low Viscosity Type

25990-25 500ML
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Label 100X100 Flammable Liquid.. - EACH

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20L TSB animal free Liquid - EACH

46-060-LB EACH
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Paraffin Liquid, High Viscosity Type

25991-15 500ML
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LN20 Liquid Nitrogen Generator - EACH

SLS6150 EACH
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SLS6154 EACH
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LN65 Liquid Nitrogen Generator - EACH

SLS6158 EACH
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Paraffin liquid, For IR Spectroscopy

GRM1136-100ML 1 unit
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Paraffin liquid, For IR Spectroscopy

GRM1136-500ML 1 unit
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Ham's F-12 with L-Gln, liquid

17458-65 500ML
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Human ESD Recombinant Protein Liquid

IHUESDESDR100UG each
EUR 721
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Human KHK Recombinant Protein Liquid

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Human PXF Recombinant Protein Liquid

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Human p53 Recombinant Protein Liquid

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Human LXN Recombinant Protein Liquid

IHULXNLXNR100UG each
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Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
Keywords: C2BBe1; SH-SY5Y; fetal bovine serum; neurospheres; xeno-free.