Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.

FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.

Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner
Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Human Cells Grow With or Without Replacement for Fetal Bovine Serum

security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.

Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.

The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).

Media Optimization Kit™, Liquid

128 kit
EUR 140

APF Media Optimization Kit™, Liquid

129 kit
EUR 140

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-250A-1 250 ml
EUR 893
  • Category: Exosomes

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-50A-1 50 ml
EUR 255
  • Category: Exosomes

FBS (PREMIUM)

35-015-CV 500 mL/pk
EUR 556
Description: Serum; Serum

FBS Replacement

0454-50 50 ml
EUR 81

FBS Replacement

0454-500 500 ml
EUR 207

Dialyzed FBS, sterile

427 100 ml
EUR 327

FBS, Transfection Optimized

F0640-050 500ml
EUR 752

FBS, Hepatocyte Optimized

F0692-050 500ml Ask for price

FBS, Keratinocyte Optimized

F0694-050 500ml Ask for price

FBS, Melanocyte Optimized

F0695-050 500ml Ask for price

Recombinant Fibrosin (FBS)

4-RPC473Hu01
  • EUR 427.94
  • EUR 217.00
  • EUR 1329.76
  • EUR 509.92
  • EUR 919.84
  • EUR 349.00
  • EUR 3174.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9HAH7
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 30.1kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Fibrosin expressed in: E.coli

Recombinant Fibrosin (FBS)

4-RPC473Mu01
  • EUR 454.82
  • EUR 224.00
  • EUR 1430.56
  • EUR 543.52
  • EUR 987.04
  • EUR 367.00
  • EUR 3426.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q8R089
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 28.8kDa
  • Isoelectric Point: 10.1
Description: Recombinant Mouse Fibrosin expressed in: E.coli

Mounting Media

99-MED 3 ml
EUR 211
Description: Mounting media for use in microscopy preparations

Grace's Media

CM021-050 500ml
EUR 85

Grace's Media

CM021-300 6x500ml
EUR 165

Grace's Media

CM021-310 10x500ml
EUR 219

Grace's Media

CM021-320 20x500ml
EUR 340

Grace's Media

CM021-350 50x500ml
EUR 585

Grace's Media

CMP21-001 10x1L
EUR 159

Grace's Media

CMP21-010 10L
EUR 146

Grace's Media

CMP21-050 50L
EUR 336

BenzAlt? Binuclease (Liquid)

9215-10
EUR 169

BenzAlt? Binuclease (Liquid)

9215-50
EUR 370

PNPP Liquid Substrate

F008-100 100 ml
EUR 234
  • Product category: Quality control/standard for molecular biology applications
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

PNPP Liquid Substrate

F008-1000 1000 ml
EUR 742
  • Product category: Quality control/standard for molecular biology applications
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

Sunflower lecithin, liquid

GE1230-100G 100 g
EUR 58

Sunflower lecithin, liquid

GE1230-1KG 1 kg
EUR 175

Sunflower lecithin, liquid

GE1230-500G 500 g
EUR 113

alpha-Amylase, liquid

GK2343-25ML 25 ml
EUR 102

FBS (PREMIUM, HEAT INACTIVATED)

35-016-CV 500 mL/pk
EUR 565
Description: Serum; Serum

FBS, Fibroblasts Optimized I

F0691-050 500ml Ask for price

FBS - Fetal Bovine Serum

FBS001 500 ml
EUR 545

FBS Alternative (Xeno-free)

P766-50 NULL
EUR 0

FBS Alternative (Xeno-free)

P766-500 NULL
EUR 0

FluorLast? Mounting Media

1213-20
EUR 142

NSC maintenance Media

ASE-9234SM 100 ml
EUR 131
Description: 18 month

Neuron Induction Media

ASE-9321NI 100 ml
EUR 131
Description: 18 month

Neuron Maturation Media

ASE-9321NM 100 ml
EUR 131
Description: 18 month

Astro Induction Media

ASE-9322AI 100 ml
EUR 131
Description: 18 month

Astro Maturation Media

ASE-9322AM 100 ml
EUR 131
Description: 18 month

DOPA Induction Media

ASE-9323DI 100 ml
EUR 147.2
Description: 18 month

DOPA Maturation Media

ASE-9323DM 100 ml
EUR 155.3
Description: 18 month

Ceraplex Media 199

CCM37-050 500ml Ask for price

Endothelial Cryopreserve Media

FM300 10 ml
EUR 257

ENDO-Growth Media

MED001 500 ml
EUR 362

ENDO Basal Media

MED002 500 ml
EUR 207

YP BASE MEDIA

Y25-110-10kg 10 kg
EUR 899

YP BASE MEDIA

Y25-110-2kg 2kg
EUR 235

YP BASE MEDIA

Y25-110-500g 500 g
EUR 100

Human Chondrocytes Media

PC00B3 500 ml
EUR 362

Pericyte-Growth Media

PGB001 500 ml
EUR 297

Pericyte Basal Media

PGB002 475 ml
EUR 271

Astrocyte-Growth Media

PGB003 500 ml
EUR 297

Astrocyte Basal Media

PGB004 500 ml
EUR 271

Protein A (Liquid form)

6500-100
EUR 729

Protein A (Liquid form)

6500-1000
EUR 1871

Protein A (Liquid form)

6500-25
EUR 262

Gamborg's Vitamin Stcks, Liquid

CP049-010 125 ml bottle
EUR 107

FRIIS BASE Medium, Liquid

F2485-100 100ml Ask for price

FRIIS BASE Medium, Liquid

F2485-500 500ml Ask for price

Protein A (Liquid form)

P1088-10 10 mg
EUR 168
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-100 100 mg
EUR 877
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-1000 1 g
EUR 2269
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-25 25 mg
EUR 308
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

EPO alpha, Liquid, human

RC213-15L 500IU
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Cytokines

FBS, Endothelial Cell Optimized I

F0670-050 500ml Ask for price

FBS, Endothelial Cell Optimized II

F0672-050 500ml Ask for price

FBS, Epithelical Cell Optimized I

F0680-050 500ml Ask for price

FBS, Epithelical Cell Optimized II

F0682-050 500ml Ask for price

FBS Opti-Gold, US Origin

F0900-050 500ml
EUR 563

Fibrosin (FBS) Polyclonal Antibody (Mouse)

4-PAC473Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: FBS (Gly11~Pro247)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS)

Human Fibrosin(FBS)ELISA Kit

QY-E00902 96T
EUR 361

Media 199 All-in-One Complete Media(10X), Singel quote

CAM37-005 50ml
EUR 246

MC-Easy Media Bonus Pack (includes Growth and Induction Media)

MN950A-1 5 preps
EUR 275
  • Category: Minicircle Technology

Dream Media Optimization Kit

184 Kit
EUR 162

Media Optimization Kit™

100 kit
EUR 122

CytoPort, Cytology Transport Media

CTM500 500 ml
EUR 71

CytoPort, Cytology Transport Media

CTM999 1000 ml
EUR 87

ENDO-Growth Media Kit

EGK001 500 ml
EUR 362

Cell Freezing Media I

CA301-005 50ml
EUR 205

Cell Freezing Media II

CA302-005 50ml
EUR 228

Cell Freezing Media III

CA303-005 50ml
EUR 242

Leibovitz'a L-15 Media

CM030-050 500ml
EUR 84

Leibovitz'a L-15 Media

CM030-300 6x500ml
EUR 169

Leibovitz'a L-15 Media

CM030-310 10x500ml
EUR 203

Leibovitz'a L-15 Media

CM030-320 20x500ml
EUR 383

Leibovitz'a L-15 Media

CM030-350 50x500ml
EUR 690

Leibovitz'a L-15 Media

CM031-050 500ml
EUR 84

Leibovitz'a L-15 Media

CM031-300 6x500ml
EUR 169

Leibovitz'a L-15 Media

CM031-310 10x500ml
EUR 203

Leibovitz'a L-15 Media

CM031-320 20x500ml
EUR 383

Leibovitz'a L-15 Media

CM031-350 50x500ml
EUR 690

McCoy's 5A Media (Modified)

CM032-050 500ml
EUR 88

McCoy's 5A Media (Modified)

CM032-300 6x500ml
EUR 165

Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
Keywords: C2BBe1; SH-SY5Y; fetal bovine serum; neurospheres; xeno-free.