Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.

FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.

Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner
Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Human Cells Grow With or Without Replacement for Fetal Bovine Serum

security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.

Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.

The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).

Viral Transport Medium Liquid Amies

IGVTMA1000ML each
EUR 145
Description: Viral Transport Medium Liquid Amies

Viral Transport Medium Liquid Amies

IGVTMA500ML each
EUR 81
Description: Viral Transport Medium Liquid Amies

Exosome-depleted FBS Media Supplement

EXO-FBS-250A-1 250 ml
EUR 837

Exosome-depleted FBS Media Supplement

EXO-FBS-50A-1 50 ml
EUR 193

C1q Liquid

pro-554L 200µg
EUR 295
Description: Human Complement Component C1q

Vitamin E, liquid

GK3575-100 100
EUR 123.4

Liquid Wicks assortment, 15, XF, F,and Medium, 200 of each size

W-A1 800 WICKS
EUR 66
Description: Liquid Wicks assortment, 15, XF, F,and Medium, 200 of each size

Hayflick Medium Complete with ampicillin and Thallium, Liquid

H2486-100 100ml Ask for price

PNPP Liquid Substrate

F008-100 100 ml
EUR 280.8
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

PNPP Liquid Substrate

F008-1000 1000 ml
EUR 890.4
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

ABTS Liquid Substrate

21530043-1 100 mL
EUR 57.34

alpha-Amylase, liquid

GK2343-25ML 25 ml
EUR 122.4

alpha-Amylase, liquid

GK2343-25 25
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Desderman pure Liquid 500 mL

CLE3234 PK20
EUR 338.15

LD4 Liquid Dewar Cap

TW-D004-9C11 1 UNIT
EUR 23
Description: LD4 Liquid Dewar Cap

100mL Cellstripper Liquid

25-056-CI PK6
EUR 116.4

BenzAlt? Binuclease (Liquid)

9215-10 each
EUR 202.8

BenzAlt? Binuclease (Liquid)

9215-50 each
EUR 444

Sunflower lecithin, liquid

GE1230-100G 100 g
EUR 69.6

Sunflower lecithin, liquid

GE1230-1KG 1 kg
EUR 210

Sunflower lecithin, liquid

GE1230-500G 500 g
EUR 135.6

Sunflower lecithin, liquid

GE1230-1 1
EUR 144

Sunflower lecithin, liquid

GE1230-100 100
EUR 27.8

Sunflower lecithin, liquid

GE1230-500 500
EUR 82.3

Hanna GP Liquid Probe

THE4525 EACH
EUR 118.8

SDS, 20% Liquid Solution

18-234 500ml/Unit
EUR 334

SDS, 20% Liquid Solution

18-234B 1000ml/Unit
EUR 390

Protein A (Liquid form)

6500-10 each
EUR 183.6

Protein A (Liquid form)

6500-100 each
EUR 874.8

Protein A (Liquid form)

6500-1000 each
EUR 2245.2

Protein A (Liquid form)

6500-25 each
EUR 314.4

Protein A (Liquid form)

P1088-10 10 mg
EUR 201.6
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-100 100 mg
EUR 1052.4
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-1000 1 g
EUR 2722.8
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)

P1088-25 25 mg
EUR 369.6
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

EPO alpha, Liquid, human

RC213-15L 500IU
EUR 125.26

Liquid Fuel Burner 100mL

BUR4502 EACH
EUR 7.2

Liquid Master 600mm - PTFE

SAM1106 EACH
EUR 318

Liquid Master 1000mm - PTFE

SAM1107 EACH
EUR 409.2

Liquid Master 1500mm - PTFE

SAM1108 EACH
EUR 507.6

Liquid Master 2000mm - PTFE

SAM1109 EACH
EUR 576

Liquid Master 2500mm - PTFE

SAM1110 EACH
EUR 728.4

Swab Liquid MRD Viscose

SWA1124 PK100
EUR 126

1 L SSPE, 20X, LIQUID

46-021-CM 1L/pk
EUR 242.4
Description: Media Catalog; Classical Media

Aquet liquid detergent 3.8l

Z273260-1EA EACH
EUR 97.2

Recombinant Streptavidin, Liquid

AOOPP-0501 10mg
EUR 2207.45

DAB Liquid Concentrate 50X

21530109-1 10 mL
EUR 92.44

DAB Liquid Concentrate 50X

21530109-2 25 mL
EUR 137.75

DAB Liquid Concentrate 50X

21530109-3 100 mL
EUR 388.67

DAB Liquid Concentrate 10X

21530113-1 100 mL
EUR 99.4

DAB Liquid Concentrate 10X

21530113-2 500 mL
EUR 259.7

100mL glutagro Liquid 200 mM Sol

25-015-CI EACH
EUR 49.2

Mucasol Liquid Detergent 2L

HAE3204 EACH
EUR 166.44

Mucasol Liquid Detergent 5L

HAE3206 EACH
EUR 348

Gamborg's Vitamin Stcks, Liquid

CP049-010 125 ml bottle
EUR 128.4

1L TSB animal free Liquid

46-060-CM EACH
EUR 177.6

Label 100X100 Flammable Liquid..

C35AR EACH
EUR 108

20L TSB animal free Liquid

46-060-LB EACH
EUR 1434

LD4 Liquid Dewar (4 liters)

TW-LD4 4 liter
EUR 808
Description: LD4 Liquid Dewar (4 liters)

LD5 Liquid Dewar (5 liters)

TW-LD5 5 liter
EUR 1049
Description: LD5 Liquid Dewar (5 liters)

LN20 Liquid Nitrogen Generator

SLS6150 EACH
EUR 47980.8

LN30 Liquid Nitrogen Generator

SLS6154 EACH
EUR 61689.6

LN65 Liquid Nitrogen Generator

SLS6158 EACH
EUR 78825.6

100mL-Glutamine 200 mM Liquid

25-005-CI PK6
EUR 118.8

LD10 Liquid Dewar (10 liters)

TW-LD10 10 liter
EUR 748
Description: LD10 Liquid Dewar (10 liters)

LD25 Liquid Dewar (25 liters)

TW-LD25 25 liter
EUR 1155
Description: LD25 Liquid Dewar (25 liters)

LD35 Liquid Dewar (35 liters)

TW-LD35 35 liter
EUR 1196
Description: LD35 Liquid Dewar (35 liters)

LD50 Liquid Dewar (50 Liters)

TW-LD50 50 liter
EUR 1612
Description: LD50 Liquid Dewar (50 Liters)

IceOff Liquid Nitrogen Drizzler

API-IceOff-01 each
EUR 324
Description: IceOff Liquid Nitrogen Drizzler

IceOff Liquid Nitrogen Drizzler

TE 1 UNIT
EUR 324
Description: IceOff Liquid Nitrogen DrizzlereNTER TESTNO NIE

1 L TE BUFFER, 1X, LIQUID

46-009-CM 1L/pk
EUR 157.2
Description: Media Catalog; Cell Culture Reagents

Automatic Liquid Nitrogen Filler

M-CEM-37454 1 UNIT
EUR 3850
Description: Automatic Liquid Nitrogen Filler

LN20AC Liquid Nitrogen Generator

SLS6148 EACH
EUR 70402.8

LN30AC Liquid Nitrogen Generator

SLS6152 EACH
EUR 65116.8

LN65AC Liquid Nitrogen Generator

SLS6156 EACH
EUR 85680

Surface liquid thermometer probe

THE0818 EACH
EUR 98.4

Liquid Wicks, Size 15, 200 /pk

W-15 200 WICKS
EUR 22
Description: Liquid Wicks, Size 15, 200 /pk

Buffer Sachet Liquid pH4 20ml

PHB1391 PK25
EUR 55.2

Buffer Sachet Liquid Ph7 25ml

PHB1392 PK25
EUR 60

Recombinant Rat IL-2, Liquid

REILP-0202 5ug Ask for price

TRITON2 Liquid Nitrogen Generator

SLS6142 EACH
EUR 40215.09

1 L TBE BUFFER, 10X, LIQUID

46-011-CM 1L/pk
EUR 141.6
Description: Media Catalog; Classical Media

TMB Liquid Substrate, for ELISA

F027-100 100 ml
EUR 262.8
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.

TMB Liquid Substrate, for ELISA

F100027 1000 ml
EUR 817.2
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.

Buffer Sachet Liquid pH10 20mL

PHB1393 PK25
EUR 60

Cryoscope Bath Liquid 500ml Dairy Testing

DAI1158 EACH
EUR 109.2

Liquid Wicks, Size Fine, 200/pk

W-F 200 WICKS
EUR 22
Description: Liquid Wicks, Size Fine, 200/pk

Julabo Liquid Level Cooling Set

BAT5234 EACH
EUR 361.2

G418 SULPHATE (LIQUID, 50 MG/ML)

30-234-CR 20 mL/pk
EUR 116.4
Description: Media Catalog; Cell Culture Reagents

Liquid Wicks, Extra Fine, 200/pk

W-XF 200 WICKS
EUR 22
Description: Liquid Wicks, Extra Fine, 200/pk

Influenza A (H1N1) Antigen Liquid

IFLUAH1N1ALR1ML each
EUR 771
Description: Influenza A (H1N1) Antigen Liquid

Influenza A (H3N2) Antigen Liquid

IFLUAH3N2ALR1ML each
EUR 754
Description: Influenza A (H3N2) Antigen Liquid

TE Buffer 10X, Liquid Concentrate

10530029-1 100 mL
EUR 18.02

TE Buffer 10X, Liquid Concentrate

10530029-2 500 mL
EUR 43

TE Buffer 10X, Liquid Concentrate

10530029-3 250 mL
EUR 27.17

TE Buffer 10X, Liquid Concentrate

10530029-4 1 L
EUR 93.89

Probe Liquid pH Std with Cable

PHC30101 EACH
EUR 369.6

RPMI 1640 without L-Gln, liquid

05176-25 500ML
EUR 10.5

10mL Gentamicin Sulfate Liquid 5

30-005-CR PK10
EUR 578.4

SSC Buffer - 20x Liquid Concentrate

D0623 1L
EUR 74.62

TAE Buffer 10X, Liquid Concentrate

10530012-1 1 L
EUR 51.77

TAE Buffer 10X, Liquid Concentrate

10530012-2 4 L
EUR 157.04

TAE Buffer 25X, Liquid Concentrate

10530013-1 1 L
EUR 44.84

TAE Buffer 25X, Liquid Concentrate

10530013-2 4 L
EUR 127.23

TAE Buffer 50X, Liquid Concentrate

10530016-1 500 mL
EUR 43

TAE Buffer 50X, Liquid Concentrate

10530016-2 1 L
EUR 69.32

TAE Buffer 50X, Liquid Concentrate

10530016-3 4 L
EUR 170.21

TBE Buffer Liquid Concentrate 10X

10530017-1 1 L
EUR 43.83

TBE Buffer Liquid Concentrate 10X

10530017-2 4 L
EUR 120.2

TBE Buffer Liquid Concentrate 10X

10530017-3 500 mL
EUR 28.07

TBE Buffer Liquid Concentrate 25X

10530022-1 1 L
EUR 65.31

TBE Buffer Liquid Concentrate 25X

10530022-2 4 L
EUR 224.93

SSC Buffer Liquid Concentrate 20X

10750018-1 1 L
EUR 59.66

SSC Buffer Liquid Concentrate 20X

10750018-2 4 L
EUR 112

SSC Buffer Liquid Concentrate 20X

10750018-3 20 L
EUR 349.19

TBS Buffer Liquid Concentrate 20X

42020064-1 4 L
EUR 102.65

TBS Buffer Liquid Concentrate 20X

42020064-2 1 L
EUR 29.83

TBS Buffer Liquid Concentrate 20X

42020064-3 6 L
EUR 146.75

SSPE Buffer Liquid Concentrate 20X

10750024-1 1 L
EUR 49.13
Description: Saline-Sodium Phosphate-EDTA Buffer

SSPE Buffer Liquid Concentrate 20X

10750024-2 4 L
EUR 165.83
Description: Saline-Sodium Phosphate-EDTA Buffer

100mL G418 Sulphate liquid 50 m

30-234-CI EACH
EUR 568.8

Cryoscope Bath Liquid Dairy Testing

CRYBL 500ML
EUR 31.2

Human KHK Recombinant Protein Liquid

IHUKHKKHKR50UG each
EUR 721
Description: Human KHK Recombinant Protein Liquid

Human p53 Recombinant Protein Liquid

IHUP53R25UG each
EUR 681
Description: Human p53 Recombinant Protein Liquid

Human p62 Recombinant Protein Liquid

IHUP62R25UG each
EUR 681
Description: Human p62 Recombinant Protein Liquid

Human PEF Recombinant Protein Liquid

IHUPEFPEFR50UG each
EUR 899
Description: Human PEF Recombinant Protein Liquid

Human PXF Recombinant Protein Liquid

IHUPXFPXFR100UG each
EUR 899
Description: Human PXF Recombinant Protein Liquid

Human LXN Recombinant Protein Liquid

IHULXNLXNR100UG each
EUR 1110
Description: Human LXN Recombinant Protein Liquid

Human ESD Recombinant Protein Liquid

IHUESDESDR100UG each
EUR 721
Description: Human ESD Recombinant Protein Liquid

Classic 25 Liquid Dewar (25 Liters)

TW-LD25C 25 liter
EUR 1305
Description: Classic 25 Liquid Dewar (25 Liters)

Human SGTA Recombinant Protein Liquid

IHUSGTASGTAR100UG each
EUR 802
Description: Human SGTA Recombinant Protein Liquid

Human SNX5 Recombinant Protein Liquid

IHUSNX5SNX5R50UG each
EUR 721
Description: Human SNX5 Recombinant Protein Liquid

Human TDO2 Recombinant Protein Liquid

IHUTDO2TDO2R50UG each
EUR 681
Description: Human TDO2 Recombinant Protein Liquid

Human PFN2 Recombinant Protein Liquid

IHUPFN2PFN2R50UG each
EUR 899
Description: Human PFN2 Recombinant Protein Liquid

Human CALR Recombinant Protein Liquid

IHUCALRCALRR100UG each
EUR 1069
Description: Human CALR Recombinant Protein Liquid

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-250A-1 250 ml
EUR 850

Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified      

EXO-FBSHI-50A-1 50 ml
EUR 206

TRITON2 PLUS Liquid Nitrogen Generator

SLS6144 EACH
EUR 43868.16

Human ACTG1 Recombinant Protein Liquid

IHUACTG1ACTG1R50UG each
EUR 721
Description: Human ACTG1 Recombinant Protein Liquid

Human POLE3 Recombinant Protein Liquid

IHUPOLE3POLE3R100UG each
EUR 899
Description: Human POLE3 Recombinant Protein Liquid

Human PPIL1 Recombinant Protein Liquid

IHUPPIL1PPIL1R100UG each
EUR 721
Description: Human PPIL1 Recombinant Protein Liquid

Human PSAT1 Recombinant Protein Liquid

IHUPSAT1PSAT1R100UG each
EUR 899
Description: Human PSAT1 Recombinant Protein Liquid

Human PSMC6 Recombinant Protein Liquid

IHUPSMC6PSMC6R50UG each
EUR 721
Description: Human PSMC6 Recombinant Protein Liquid

Human MAT2A Recombinant Protein Liquid

IHUMAT2AMAT2AR100UG each
EUR 899
Description: Human MAT2A Recombinant Protein Liquid

Human DNAL4 Recombinant Protein Liquid

IHUDNAL4DNAL4R100UG each
EUR 899
Description: Human DNAL4 Recombinant Protein Liquid

Hellma(R) liquid calibration standards

Z803251-1EA EACH
EUR 782.05

Human MTHFD2 Recombinant Protein Liquid

IHUMTHFD2MTHFD2R50UG each
EUR 632
Description: Human MTHFD2 Recombinant Protein Liquid

Human PPP1CC Recombinant Protein Liquid

IHUPPP1CCPPP1CCR20UG each
EUR 899
Description: Human PPP1CC Recombinant Protein Liquid

LN20AC Compact Liquid Nitrogen Generator

SLS6146 EACH
EUR 67269.6

Glycerol Assay Kit (For liquid samples)

abx090674-1Kit 1 Kit
EUR 735.6

HIV-1 gD Recombinant Protein liquid

IHIV1HSV1GDR10UG each
EUR 948
Description: HIV-1 gD Recombinant Protein liquid

Human ATP6V1E1 Recombinant Protein Liquid

IHUATP6V1E1ATP6V1E1R50UG each
EUR 681
Description: Human ATP6V1E1 Recombinant Protein Liquid

Human Survivin Recombinant Protein Liquid

IHUBIRC5R25UG each
EUR 681
Description: Human Survivin Recombinant Protein Liquid

HIV-1 p24 Recombinant Protein liquid

IHIV1HIV1P24R10UG each
EUR 948
Description: HIV-1 p24 Recombinant Protein liquid

WHEATON(R) LIQUID SCINTILLATION VIAL WI

DWK986561-500EA PK500
EUR 346.8

WHEATON(R) LIQUID SCINTILLATION VIAL WI

DWK986754-500EA PK500
EUR 207.6

HIV-1 gp41 Recombinant Protein liquid

IHIV1HIV1GP41R10UG each
EUR 762
Description: HIV-1 gp41 Recombinant Protein liquid

HIV-2 gp36 Recombinant Protein liquid

IHIV2HIV2GP36R10UG each
EUR 851
Description: HIV-2 gp36 Recombinant Protein liquid

Murashige and Skoog Vitamin Stock; Liquid

CP050-010 100 ml bottle
EUR 128.4

Human IMP-1 Recombinant Protein Liquid

IHUIMP1R25UG each
EUR 681
Description: Human IMP-1 Recombinant Protein Liquid

Human IMP-3 Recombinant Protein Liquid

IHUIMP3R25UG each
EUR 681
Description: Human IMP-3 Recombinant Protein Liquid

HELLMA UV CALIBRATION COMPLETE LIQUID FI

Z802204-1EA EACH
EUR 5651.94

Glass bottle for calibration liquid 50ml

WAT4876 EACH
EUR 238.92

TB Buffer 10X, pH 7.0, Liquid Concentrate

42020410-1 1 L
EUR 49.97

TB Buffer 10X, pH 7.0, Liquid Concentrate

42020410-2 500 mL
EUR 35.73

50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION

30-006-CF 50 mL/pk
EUR 117.6
Description: Media Catalog; Cell Culture Reagents

Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
Keywords: C2BBe1; SH-SY5Y; fetal bovine serum; neurospheres; xeno-free.