Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.
FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.
Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.
Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner Human Cells Grow With or Without Replacement for Fetal Bovine Serum
security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.
Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.
The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).
FBS (PREMIUM)
35-015-CV
CORNING
500 mL/pk
EUR 556
Description: Serum; Serum
FBS Replacement
0454-50
AthenaES
50 ml
EUR 81
FBS Replacement
0454-500
AthenaES
500 ml
EUR 207
Recombinant Fibrosin (FBS)
4-RPC473Hu01
Cloud-Clone
EUR 427.94
EUR 217.00
EUR 1329.76
EUR 509.92
EUR 919.84
EUR 349.00
EUR 3174.40
100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Fibrosin expressed in: E.coli
Recombinant Fibrosin (FBS)
4-RPC473Mu01
Cloud-Clone
EUR 454.82
EUR 224.00
EUR 1430.56
EUR 543.52
EUR 987.04
EUR 367.00
EUR 3426.40
100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Fibrosin expressed in: E.coli
FBS, Transfection Optimized
F0640-050
GenDepot
500ml
EUR 752
FBS, Hepatocyte Optimized
F0692-050
GenDepot
500ml
Ask for price
FBS, Keratinocyte Optimized
F0694-050
GenDepot
500ml
Ask for price
FBS, Melanocyte Optimized
F0695-050
GenDepot
500ml
Ask for price
Dialyzed FBS, sterile
427
AthenaES
100 ml
EUR 327
Grace's Media
CM021-050
GenDepot
500ml
EUR 85
Grace's Media
CM021-300
GenDepot
6x500ml
EUR 165
Grace's Media
CM021-310
GenDepot
10x500ml
EUR 219
Grace's Media
CM021-320
GenDepot
20x500ml
EUR 340
Grace's Media
CM021-350
GenDepot
50x500ml
EUR 585
Grace's Media
CMP21-001
GenDepot
10x1L
EUR 159
Grace's Media
CMP21-010
GenDepot
10L
EUR 146
Grace's Media
CMP21-050
GenDepot
50L
EUR 336
Mounting Media
99-MED
Fitzgerald
3 ml
EUR 211
Description: Mounting media for use in microscopy preparations
PNPP Liquid Substrate
F008-100
Cygnus Technologies
100 ml
EUR 234
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.
PNPP Liquid Substrate
F008-1000
Cygnus Technologies
1000 ml
EUR 742
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.
BenzAlt? Binuclease (Liquid)
9215-10
Biovision
EUR 169
BenzAlt? Binuclease (Liquid)
9215-50
Biovision
EUR 370
FBS, Fibroblasts Optimized I
F0691-050
GenDepot
500ml
Ask for price
FBS - Fetal Bovine Serum
FBS001
Neuromics
500 ml
EUR 545
FBS Alternative (Xeno-free)
P766-50
101Bio
-
Ask for price
FBS Alternative (Xeno-free)
P766-500
101Bio
-
Ask for price
FBS (PREMIUM, HEAT INACTIVATED)
35-016-CV
CORNING
500 mL/pk
EUR 565
Description: Serum; Serum
Endothelial Cryopreserve Media
FM300
Neuromics
10 ml
EUR 257
Human Chondrocytes Media
PC00B3
Neuromics
500 ml
EUR 362
Pericyte-Growth Media
PGB001
Neuromics
500 ml
EUR 297
Pericyte Basal Media
PGB002
Neuromics
475 ml
EUR 271
Astrocyte-Growth Media
PGB003
Neuromics
500 ml
EUR 297
Astrocyte Basal Media
PGB004
Neuromics
500 ml
EUR 271
ENDO-Growth Media
MED001
Neuromics
500 ml
EUR 362
ENDO Basal Media
MED002
Neuromics
500 ml
EUR 207
Ceraplex Media 199
CCM37-050
GenDepot
500ml
Ask for price
NSC maintenance Media
ASE-9234SM
Applied StemCell
100 ml
EUR 131
Description: 18 month
Neuron Induction Media
ASE-9321NI
Applied StemCell
100 ml
EUR 131
Description: 18 month
Neuron Maturation Media
ASE-9321NM
Applied StemCell
100 ml
EUR 131
Description: 18 month
Astro Induction Media
ASE-9322AI
Applied StemCell
100 ml
EUR 131
Description: 18 month
Astro Maturation Media
ASE-9322AM
Applied StemCell
100 ml
EUR 131
Description: 18 month
DOPA Induction Media
ASE-9323DI
Applied StemCell
100 ml
EUR 147.2
Description: 18 month
DOPA Maturation Media
ASE-9323DM
Applied StemCell
100 ml
EUR 155.3
Description: 18 month
FluorLast? Mounting Media
1213-20
Biovision
EUR 142
EPO alpha, Liquid, human
RC213-15L
Bio Basic
500IU
EUR 104.38
FRIIS BASE Medium, Liquid
F2485-100
GenDepot
100ml
Ask for price
FRIIS BASE Medium, Liquid
F2485-500
GenDepot
500ml
Ask for price
Gamborg's Vitamin Stcks, Liquid
CP049-010
GenDepot
125 ml bottle
EUR 107
Protein A (Liquid form)
P1088-100
ApexBio
100 mg
EUR 877
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.
Protein A (Liquid form)
P1088-1000
ApexBio
1 g
EUR 2269
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.
Protein A (Liquid form)
P1088-25
ApexBio
25 mg
EUR 308
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.
Protein A (Liquid form)
6500-10
Biovision
EUR 153
Protein A (Liquid form)
6500-100
Biovision
EUR 729
Protein A (Liquid form)
6500-1000
Biovision
EUR 1871
Protein A (Liquid form)
6500-25
Biovision
EUR 262
Fibrosin (FBS) Polyclonal Antibody (Mouse)
4-PAC473Mu01
Cloud-Clone
EUR 251.00
EUR 2576.00
EUR 640.00
EUR 316.00
EUR 215.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS)
FBS, Endothelial Cell Optimized I
F0670-050
GenDepot
500ml
Ask for price
FBS, Endothelial Cell Optimized II
F0672-050
GenDepot
500ml
Ask for price
FBS, Epithelical Cell Optimized I
F0680-050
GenDepot
500ml
Ask for price
FBS, Epithelical Cell Optimized II
F0682-050
GenDepot
500ml
Ask for price
FBS Opti-Gold, US Origin
F0900-050
GenDepot
500ml
EUR 563
Media 199 All-in-One Complete Media(10X), Singel quote
CAM37-005
GenDepot
50ml
EUR 246
MC-Easy Media Bonus Pack (includes Growth and Induction Media)
MN950A-1
SBI
5 preps
EUR 275
TMB Liquid Substrate, for ELISA
F027-100
Cygnus Technologies
100 ml
EUR 219
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.
TMB Liquid Substrate, for ELISA
F100027
Cygnus Technologies
1000 ml
EUR 681
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.
Phenol-liquid, saturated with water
PC5523
Bio Basic
100ml
EUR 91.76
SSC Buffer - 20x Liquid Concentrate
D0623
Bio Basic
1L
EUR 62.18
1 L SSPE, 20X, LIQUID
46-021-CM
CORNING
1L/pk
EUR 202
Description: Media Catalog; Classical Media
100 ML CELLSTRIPPER™, LIQUID
25-056-CI
CORNING
100 mL/pk
EUR 81
Description: Media Catalog; Cell Culture Reagents
MOK Prime-olate™ Liquid
179
AthenaES
Kit
EUR 165
ENDO-Growth Media Kit
EGK001
Neuromics
500 ml
EUR 362
Human BBB Model Media
NMBBB01
Neuromics
500 ml - Media
EUR 435
Cell Freezing Media I
CA301-005
GenDepot
50ml
EUR 205
Cell Freezing Media II
CA302-005
GenDepot
50ml
EUR 228
Cell Freezing Media III
CA303-005
GenDepot
50ml
EUR 242
Leibovitz'a L-15 Media
CM030-050
GenDepot
500ml
EUR 84
Leibovitz'a L-15 Media
CM030-300
GenDepot
6x500ml
EUR 169
Leibovitz'a L-15 Media
CM030-310
GenDepot
10x500ml
EUR 203
Leibovitz'a L-15 Media
CM030-320
GenDepot
20x500ml
EUR 383
Leibovitz'a L-15 Media
CM030-350
GenDepot
50x500ml
EUR 690
Leibovitz'a L-15 Media
CM031-050
GenDepot
500ml
EUR 84
Leibovitz'a L-15 Media
CM031-300
GenDepot
6x500ml
EUR 169
Leibovitz'a L-15 Media
CM031-310
GenDepot
10x500ml
EUR 203
Leibovitz'a L-15 Media
CM031-320
GenDepot
20x500ml
EUR 383
Leibovitz'a L-15 Media
CM031-350
GenDepot
50x500ml
EUR 690
McCoy's 5A Media
(Modified)
CM032-050
GenDepot
500ml
EUR 88
McCoy's 5A Media
(Modified)
CM032-300
GenDepot
6x500ml
EUR 165
McCoy's 5A Media
(Modified)
CM032-310
GenDepot
10x500ml
EUR 219
McCoy's 5A Media
(Modified)
CM032-320
GenDepot
20x500ml
EUR 340
McCoy's 5A Media
(Modified)
CM032-350
GenDepot
50x500ml
EUR 585
McCoy's 5A Media
(Modified)
CM033-050
GenDepot
500ml
EUR 85
McCoy's 5A Media
(Modified)
CM033-300
GenDepot
6x500ml
EUR 165
McCoy's 5A Media
(Modified)
CM033-310
GenDepot
10x500ml
EUR 219
McCoy's 5A Media
(Modified)
CM033-320
GenDepot
20x500ml
EUR 340
McCoy's 5A Media
(Modified)
CM033-350
GenDepot
50x500ml
EUR 585
Media Optimization Kit™
100
AthenaES
kit
EUR 122
Dream Media Optimization Kit
184
AthenaES
Kit
EUR 162
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse)
4-PAC473Hu01
Cloud-Clone
EUR 247.00
EUR 2510.00
EUR 625.00
EUR 310.00
EUR 214.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS)
Fibrosin (FBS) Polyclonal Antibody (Mouse), APC
4-PAC473Mu01-APC
Cloud-Clone
EUR 351.00
EUR 3365.00
EUR 935.00
EUR 449.00
EUR 222.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with APC.
Fibrosin (FBS) Polyclonal Antibody (Mouse), Biotinylated
4-PAC473Mu01-Biotin
Cloud-Clone
EUR 316.00
EUR 2526.00
EUR 744.00
EUR 387.00
EUR 221.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with Biotin.
Fibrosin (FBS) Polyclonal Antibody (Mouse), Cy3
4-PAC473Mu01-Cy3
Cloud-Clone
EUR 427.00
EUR 4445.00
EUR 1205.00
EUR 557.00
EUR 254.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with Cy3.
Fibrosin (FBS) Polyclonal Antibody (Mouse), FITC
4-PAC473Mu01-FITC
Cloud-Clone
EUR 301.00
EUR 2712.00
EUR 768.00
EUR 379.00
EUR 197.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with FITC.
Fibrosin (FBS) Polyclonal Antibody (Mouse), HRP
4-PAC473Mu01-HRP
Cloud-Clone
EUR 321.00
EUR 2933.00
EUR 827.00
EUR 405.00
EUR 209.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with HRP.
Fibrosin (FBS) Polyclonal Antibody (Mouse), PE
4-PAC473Mu01-PE
Cloud-Clone
EUR 301.00
EUR 2712.00
EUR 768.00
EUR 379.00
EUR 197.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with PE.
FBS - Fetal Bovine Serum USDA Origin
FBS002
Neuromics
500 mL
EUR 479
Fetal Bovine Serum (FBS) [50 ml]
ABP-MED-FBS0050
Allele Biotech
50ml
Ask for price
Fetal Bovine Serum (FBS) [500 ml]
ABP-MED-FBS0500
Allele Biotech
500ml
Ask for price
Murashige and Skoog Vitamin Stock; Liquid
CP050-010
GenDepot
100 ml bottle
EUR 107
1 L TE BUFFER, 1X, LIQUID
46-009-CM
CORNING
1L/pk
EUR 131
Description: Media Catalog; Cell Culture Reagents
1 L TBE BUFFER, 10X, LIQUID
46-011-CM
CORNING
1L/pk
EUR 118
Description: Media Catalog; Classical Media
Glycerol Assay Kit (For liquid samples)
abx090674-1Kit
Abbexa
1 Kit
EUR 613
100 ML-GLUTAMINE (200 MM, LIQUID)
25-005-CI
CORNING
100 mL/pk
EUR 58
Description: Media Catalog; Cell Culture Reagents
G418 SULPHATE (LIQUID, 50 MG/ML)
30-234-CI
CORNING
100 mL/pk
EUR 297
Description: Media Catalog; Cell Culture Reagents
G418 SULPHATE (LIQUID, 50 MG/ML)
30-234-CR
CORNING
20 mL/pk
EUR 97
Description: Media Catalog; Cell Culture Reagents
Human L-EGF (Media Grade)
NM001
GroPep
1 mg
EUR 438
Human L-EGF (Media Grade)
NM005
GroPep
5 mg
EUR 717
Human IGF-II (Media Grade)
OM001
GroPep
1 mg
EUR 902
Human IGF-II (Media Grade)
OU100
GroPep
100 µg
EUR 327
Human IGF-I (Media Grade)
IM001
GroPep
1 mg
EUR 624
Human IGF-I (Media Grade)
IM005
GroPep
5 mg
EUR 161
Human IGF-I (Media Grade)
IU100
GroPep
100 µg
EUR 327
Pancreatic Stellate CAF Maintaining Media
PC00B5
Neuromics
500 ml
EUR 321
Mouse ES Cell Culture Media
M16101
Neuromics
500 mL
EUR 1114
Rat Neurosphere-Progenitors Proliferation Media
M36111
Neuromics
500 ml
EUR 826
CMRL 1066, CIT CULTURE MEDIA
98-304-CV
CORNING
500 mL/pk
EUR 65
Description: Specialty Media; Islet products
ExoPure? Isolation Kit (Cell Media)
K1237-10
Biovision
EUR 664
ExoPure? Isolation Kit (Cell Media)
K1237-2
Biovision
EUR 392
APF Media Optimization Kit™
0100-APF
AthenaES
kit
EUR 122
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), APC
4-PAC473Hu01-APC
Cloud-Clone
EUR 345.00
EUR 3275.00
EUR 912.00
EUR 440.00
EUR 219.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with APC.
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), Biotinylated
4-PAC473Hu01-Biotin
Cloud-Clone
EUR 311.00
EUR 2460.00
EUR 727.00
EUR 381.00
EUR 219.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with Biotin.
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), Cy3
4-PAC473Hu01-Cy3
Cloud-Clone
EUR 419.00
EUR 4325.00
EUR 1175.00
EUR 545.00
EUR 251.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with Cy3.
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), FITC
4-PAC473Hu01-FITC
Cloud-Clone
EUR 296.00
EUR 2640.00
EUR 750.00
EUR 372.00
EUR 195.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with FITC.
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), HRP
4-PAC473Hu01-HRP
Cloud-Clone
EUR 316.00
EUR 2855.00
EUR 807.00
EUR 398.00
EUR 206.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with HRP.
Fibrosin (FBS) Polyclonal Antibody (Human, Mouse), PE
4-PAC473Hu01-PE
Cloud-Clone
EUR 296.00
EUR 2640.00
EUR 750.00
EUR 372.00
EUR 195.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS). This antibody is labeled with PE.
Fibrosin (FBS) Polyclonal Antibody (Mouse), APC-Cy7
4-PAC473Mu01-APC-Cy7
Cloud-Clone
EUR 583.00
EUR 6610.00
EUR 1750.00
EUR 778.00
EUR 324.00
100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with APC-Cy7.
FBS Ultra Low IgG, < 5 μg/ml
F1900-050
GenDepot
500ml
EUR 928
FBS-HI - Heat Inactivated Fetal Bovine Serum
FBS001-HI
Neuromics
500 ml
EUR 564
2-Mercaptoethanol (1000X), liquid, 55mM in DPBS
CA713-005
GenDepot
50 ml
EUR 120
1000UL TECAN-STYLE LIQUID LEVEL SENSING TIP.
TT-1000-CBK-HTR
CORNING
1536/pk
EUR 136
Description: Robotic Tips; Robotic tips - Axygen
20UL TECAN-STYLE LIQUID LEVEL SENSING TIP
TT-20-CBK-HTR
CORNING
2304/pk
EUR 201
Description: Robotic Tips; Robotic tips - Axygen
200UL TECAN-STYLE LIQUID LEVEL SENSING TIP.
TT-200-CBK-HTR
CORNING
2304/pk
EUR 195
Description: Robotic Tips; Robotic tips - Axygen
50UL TECAN-STYLE LIQUID LEVEL SENSING TIP.
TT-50-CBK-HTR
CORNING
2304/pk
EUR 201
Description: Robotic Tips; Robotic tips - Axygen
THERMALTRAY SLP LOW PROFILE FOR LIQUID NITROGEN
432073
CORNING
1/pk
EUR 332
Description: BioCision; BioCision CoolBox
1 L TRIS BUFFERED SALINE, 10X, LIQUID
46-012-CM
CORNING
1L/pk
EUR 97
Description: Media Catalog; Buffered Salt-Salution
TSB (TRYPTIC SOY BROTH), ANIMAL FREE, LIQUID
46-060-CM
CORNING
1L/pk
EUR 72
Description: Media Catalog; Classical Media
Human [Arg3] IGF-I (Media Grade)
LM001
GroPep
1 mg
EUR 624
Human [Arg3] IGF-I (Media Grade)
LM005
GroPep
5 mg
EUR 161
Human [Arg3] IGF-I (Media Grade)
LU100
GroPep
100 µg
EUR 299
Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
