Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Fetal bovine serum (FBS) containing a large amount of exosomes that can interfere with the analysis of exosomes derived from cultured cells. We investigated the effect of exosomes derived FBS (FBS-Exos) on adipogenic differentiation of human bone marrow mesenchymal stromal cells (HBM-MSCs) and the underlying molecular mechanisms.

FBS-Exos absorption by HBM-MSCs can be detected by laser scanning confocal microscope, and the treatment of exosomes resulted in a decrease in lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of HBM-MSCs. miR-1246 was identified as microRNA is abundant in FBS-Exos by public identification data sequencing and RT-qPCR validation. In addition, miR-1246 in the HBM-MSCs excess causes decreased levels of adipogenic differentiation, whereas miR-1246 in FBS-Exos knockdown attenuated inhibitory effects on adipogenic differentiation.

Our results indicate that FBS-Exos inhibits adipogenic differentiation of HBM-MSCs by means of cross-species and miR-1246 transferred by FBS-Exos partly contribute to this effect.

Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner
Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner

Human Cells Grow With or Without Replacement for Fetal Bovine Serum

security problems on cell-derived pharmaceutical products manufactured in the supplement of fetal bovine serum (FBS) has fueled the demand to replace FBS.

Here, four new alternative to FBS marketed compared: xeno-free product called Ess® cells, human platelet lysates marketed as GroPro®, and two mixtures of serum adult cattle vary in their proportion of neonatal growth factor, called Liporo® and FetalGro®. Endothelial cell line (C2BBe1) and neural cell lines (SHSY5Y) near confluency media with 10% FBS is selectively etched and taken through a step-wise algorithm 25 days to replace FBS, and other human endothelial cell line (HRA- 19) studied for C2BBe1 emulate. Cells were stained and counted and compared to the survival, migration, and spheroids.

The C2BBe1 and HRA-19 cell line failed to proliferate in 10% of cells Ess® but growing at 10% or 10% FetalGro® GroPro® quite good compared with 10% FBS reference. The SH-SY5Y, just FetalGro® FBS efficacy of this approach. These are all inferior to 11 different branded many of FBS (positive control), but five days to switch only between brands FBS, 4 out of 11 supported less proliferation of endothelial FBS reference in HRA-19 (p <0.004). In addition, the neurospheres were enriched in two many brands of FBS and FetalGro® (each p <0.004), neurospheres be undesirable phenotype for nerve cell applications. Because GroPro® platelet-derived growth supplement prominent among non-FBS to allow proliferation without going spheroids, it seems the best (be aware that the cells still grew more slowly in it compared to FBS).

FBS Replacement
0454-500 500 ml
EUR 248.4

Dialyzed FBS, sterile
427 100 ml
EUR 392.4

FBS, Transfection Optimized
F0640-050 500ml
EUR 902.4

FBS, Hepatocyte Optimized
F0692-050 500ml Ask for price

FBS, Keratinocyte Optimized
F0694-050 500ml Ask for price

FBS, Melanocyte Optimized
F0695-050 500ml Ask for price

Recombinant Fibrosin (FBS)
4-RPC473Hu01
  • EUR 513.53
  • EUR 260.40
  • EUR 1595.71
  • EUR 611.90
  • EUR 1103.81
  • EUR 418.80
  • EUR 3809.28
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Fibrosin expressed in: E.coli

Recombinant Fibrosin (FBS)
4-RPC473Mu01
  • EUR 545.78
  • EUR 268.80
  • EUR 1716.67
  • EUR 652.22
  • EUR 1184.45
  • EUR 440.40
  • EUR 4111.68
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Fibrosin expressed in: E.coli

FBS (PREMIUM, HEAT INACTIVATED)
35-016-CV 500 mL/pk
EUR 678
Description: Serum; Serum

FBS, Fibroblasts Optimized I
F0691-050 500ml Ask for price

FBS - Fetal Bovine Serum
FBS001 500 ml
EUR 654

FBS Alternative (Xeno-free)
P766-50 - Ask for price

FBS Alternative (Xeno-free)
P766-500 - Ask for price

100ML FBS - Sterile - Filtered
SLS1166 100ML
EUR 70.68

Mounting Media
99-MED 3 ml
EUR 253.2
Description: Mounting media for use in microscopy preparations

Grace's Media
CM021-050 500ml
EUR 102

Grace's Media
CM021-300 6x500ml
EUR 198

Grace's Media
CM021-310 10x500ml
EUR 262.8

Grace's Media
CM021-320 20x500ml
EUR 408

Grace's Media
CM021-350 50x500ml
EUR 702

Grace's Media
CMP21-001 10x1L
EUR 190.8

Grace's Media
CMP21-010 10L
EUR 175.2

Grace's Media
CMP21-050 50L
EUR 403.2

BenzAlt? Binuclease (Liquid)
9215-10
EUR 202.8

BenzAlt? Binuclease (Liquid)
9215-50
EUR 444

Sunflower lecithin, liquid
GE1230-100G 100 g
EUR 69.6

Sunflower lecithin, liquid
GE1230-1KG 1 kg
EUR 210

Sunflower lecithin, liquid
GE1230-500G 500 g
EUR 135.6

alpha-Amylase, liquid
GK2343-25ML 25 ml
EUR 122.4

PNPP Liquid Substrate
F008-100 100 ml
EUR 280.8
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

PNPP Liquid Substrate
F008-1000 1000 ml
EUR 890.4
Description: PNPP Liquid Substrate by Cygnus Technologies is available in Europe via Gentaur.

100mL Cellstripper Liquid
25-056-CI PK6
EUR 104.88

Sabouraud Liquid Medium
MED1462 EACH
EUR 54.61

LEXSY BHI - Liquid Media Kit ** shipped on blue ice - must be shipped via overnight service
M-ML-411L 5 x 1 L
EUR 532
Description: LEXSY BHI - Liquid Media Kit ** shipped on blue ice - must be shipped via overnight service

LEXSY BHI - Liquid Media Kit ** shipped on blue ice - must be shipped via overnight service
M-ML-411S 1 L
EUR 142
Description: LEXSY BHI - Liquid Media Kit ** shipped on blue ice - must be shipped via overnight service

Fibrosin (FBS) Polyclonal Antibody (Mouse)
4-PAC473Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS)

FBS, Endothelial Cell Optimized I
F0670-050 500ml Ask for price

FBS, Endothelial Cell Optimized II
F0672-050 500ml Ask for price

FBS, Epithelical Cell Optimized I
F0680-050 500ml Ask for price

FBS, Epithelical Cell Optimized II
F0682-050 500ml Ask for price

FBS Opti-Gold, US Origin
F0900-050 500ml
EUR 675.6

Human Fibrosin(FBS)ELISA Kit
QY-E00902 96T
EUR 433.2

100ML FBS - Sterile - Heat Inactivated
SLS1168 100ML
EUR 102.6

FluorLast? Mounting Media
1213-20
EUR 170.4

NSC maintenance Media
ASE-9234SM 100 ml
EUR 157.2
Description: 18 month

Neuron Induction Media
ASE-9321NI 100 ml
EUR 157.2
Description: 18 month

Neuron Maturation Media
ASE-9321NM 100 ml
EUR 157.2
Description: 18 month

Astro Induction Media
ASE-9322AI 100 ml
EUR 157.2
Description: 18 month

Astro Maturation Media
ASE-9322AM 100 ml
EUR 157.2
Description: 18 month

DOPA Induction Media
ASE-9323DI 100 ml
EUR 176.64
Description: 18 month

DOPA Maturation Media
ASE-9323DM 100 ml
EUR 186.36
Description: 18 month

Ceraplex Media 199
CCM37-050 500ml Ask for price

ENDO-Growth Media
MED001 500 ml
EUR 434.4

ENDO Basal Media
MED002 500 ml
EUR 248.4

Endothelial Cryopreserve Media
FM300 10 ml
EUR 308.4

Pericyte-Growth Media
PGB001 500 ml
EUR 356.4

Pericyte Basal Media
PGB002 475 ml
EUR 325.2

Astrocyte-Growth Media
PGB003 500 ml
EUR 356.4

Astrocyte Basal Media
PGB004 500 ml
EUR 325.2

Human Chondrocytes Media
PC00B3 500 ml
EUR 434.4

YP BASE MEDIA
Y25-110-10kg 10 kg
EUR 1078.8

YP BASE MEDIA
Y25-110-2kg 2kg
EUR 282

YP BASE MEDIA
Y25-110-500g 500 g
EUR 120

Protein A (Liquid form)
6500-10
EUR 183.6

Protein A (Liquid form)
6500-100
EUR 874.8

Protein A (Liquid form)
6500-1000
EUR 2245.2

Protein A (Liquid form)
6500-25
EUR 314.4

Gamborg's Vitamin Stcks, Liquid
CP049-010 125 ml bottle
EUR 128.4

Protein A (Liquid form)
P1088-10 10 mg
EUR 201.6
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)
P1088-100 100 mg
EUR 1052.4
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)
P1088-1000 1 g
EUR 2722.8
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

Protein A (Liquid form)
P1088-25 25 mg
EUR 369.6
Description: Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins.

FRIIS BASE Medium, Liquid
F2485-100 100ml Ask for price

FRIIS BASE Medium, Liquid
F2485-500 500ml Ask for price

EPO alpha, Liquid, human
RC213-15L 500IU
EUR 125.26

Mucasol Liquid Detergent 2L
HAE3204 EACH
EUR 166.44

Mucasol Liquid Detergent 5L
HAE3206 EACH
EUR 286.03

TRITON2 Liquid Nitrogen Generator
SLS6142 EACH
EUR 35114.28

LN20AC Liquid Nitrogen Generator
SLS6148 EACH
EUR 44887.5

LN20 Liquid Nitrogen Generator
SLS6150 EACH
EUR 41895

LN30AC Liquid Nitrogen Generator
SLS6152 EACH
EUR 56857.5

LN30 Liquid Nitrogen Generator
SLS6154 EACH
EUR 53865

LN65AC Liquid Nitrogen Generator
SLS6156 EACH
EUR 74812.5

LN65 Liquid Nitrogen Generator
SLS6158 EACH
EUR 68827.5

Liquid Fuel Burner 100mL
BUR4502 EACH
EUR 5.59

Label 100X100 Flammable Liquid..
C35AR EACH
EUR 102.6

Swab Liquid MRD Viscose
SWA1124 PK100
EUR 108.3

Surface liquid thermometer probe
THE0818 EACH
EUR 77.52

Hanna GP Liquid Probe
THE4525 EACH
EUR 87.78

Aquet liquid detergent 3.8l
Z273260-1EA EACH
EUR 67.6

Liquid Master 600mm - PTFE
SAM1106 EACH
EUR 279.3

Liquid Master 1000mm - PTFE
SAM1107 EACH
EUR 349.98

Liquid Master 1500mm - PTFE
SAM1108 EACH
EUR 434.34

Liquid Master 2000mm - PTFE
SAM1109 EACH
EUR 506.16

Liquid Master 2500mm - PTFE
SAM1110 EACH
EUR 622.44

IceOff Liquid Nitrogen Drizzler
TE 1 UNIT
EUR 324
Description: IceOff Liquid Nitrogen DrizzlereNTER TESTNO NIE

LD4 Liquid Dewar Cap
TW-D004-9C11 1 UNIT
EUR 23
Description: LD4 Liquid Dewar Cap

Automatic Liquid Nitrogen Filler
M-CEM-37454 1 UNIT
EUR 3500
Description: Automatic Liquid Nitrogen Filler

Fetal Bovine Serum (FBS) [50 ml]
ABP-MED-FBS0050 50ml Ask for price

Fetal Bovine Serum (FBS) [500 ml]
ABP-MED-FBS0500 500ml Ask for price

Fibrosin (FBS) Polyclonal Antibody (Human, Mouse)
4-PAC473Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Fibrosin (FBS)

Fibrosin (FBS) Polyclonal Antibody (Mouse), APC
4-PAC473Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with APC.

Fibrosin (FBS) Polyclonal Antibody (Mouse), Biotinylated
4-PAC473Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with Biotin.

Fibrosin (FBS) Polyclonal Antibody (Mouse), Cy3
4-PAC473Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with Cy3.

Fibrosin (FBS) Polyclonal Antibody (Mouse), FITC
4-PAC473Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with FITC.

Fibrosin (FBS) Polyclonal Antibody (Mouse), HRP
4-PAC473Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with HRP.

Fibrosin (FBS) Polyclonal Antibody (Mouse), PE
4-PAC473Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fibrosin (FBS). This antibody is labeled with PE.

FBS - Fetal Bovine Serum USDA Origin
FBS002 500 mL
EUR 574.8

South American FBS Serum 500ml Bottles
35-079-CV 500ML
EUR 391.02

500ML FBS EU Grade Origin: Brazil
SLS1108 500ML
EUR 273.6

Media 199 All-in-One Complete Media(10X), Singel quote
CAM37-005 50ml
EUR 295.2

MC-Easy Media Bonus Pack (includes Growth and Induction Media)
MN950A-1 5 preps
EUR 330

Leibovitz'a L-15 Media
CM030-050 500ml
EUR 100.8

Leibovitz'a L-15 Media
CM030-300 6x500ml
EUR 202.8

Leibovitz'a L-15 Media
CM030-310 10x500ml
EUR 243.6

Leibovitz'a L-15 Media
CM030-320 20x500ml
EUR 459.6

Leibovitz'a L-15 Media
CM030-350 50x500ml
EUR 828

Leibovitz'a L-15 Media
CM031-050 500ml
EUR 100.8

Leibovitz'a L-15 Media
CM031-300 6x500ml
EUR 202.8

Leibovitz'a L-15 Media
CM031-310 10x500ml
EUR 243.6

Leibovitz'a L-15 Media
CM031-320 20x500ml
EUR 459.6

Leibovitz'a L-15 Media
CM031-350 50x500ml
EUR 828

McCoy's 5A Media (Modified)
CM032-050 500ml
EUR 105.6

McCoy's 5A Media (Modified)
CM032-300 6x500ml
EUR 198

McCoy's 5A Media (Modified)
CM032-310 10x500ml
EUR 262.8

McCoy's 5A Media (Modified)
CM032-320 20x500ml
EUR 408

McCoy's 5A Media (Modified)
CM032-350 50x500ml
EUR 702

McCoy's 5A Media (Modified)
CM033-050 500ml
EUR 102

McCoy's 5A Media (Modified)
CM033-300 6x500ml
EUR 198

McCoy's 5A Media (Modified)
CM033-310 10x500ml
EUR 262.8

McCoy's 5A Media (Modified)
CM033-320 20x500ml
EUR 408

McCoy's 5A Media (Modified)
CM033-350 50x500ml
EUR 702

CytoPort, Cytology Transport Media
CTM500 500 ml
EUR 85.2

CytoPort, Cytology Transport Media
CTM999 1000 ml
EUR 104.4

Cell Freezing Media I
CA301-005 50ml
EUR 246

Cell Freezing Media II
CA302-005 50ml
EUR 273.6

Cell Freezing Media III
CA303-005 50ml
EUR 290.4

Human BBB Model Media
NMBBB01 500 ml - Media
EUR 522

ENDO-Growth Media Kit
EGK001 500 ml
EUR 434.4

Buffered Glycerol Mounting Media
GMM003 3 ml
EUR 73.2

Buffered Glycerol Mounting Media
GMM030 30 ml
EUR 86.4

Buffered Glycerol Mounting Media
GMM500 500 ml
EUR 219.6

Buffered Glycerol Mounting Media
GMM999 1000 ml
EUR 312

Permanent Mounting Media (Aqueous)
PMT030 30 ml
EUR 130.8

Permanent Mounting Media (Aqueous)
PMT999 1000 ml
EUR 910.8

Autoclave Classic Media 9L
AUT4515 EACH
EUR 1413.6

Autoclave Classic Media 12L
AUT4517 EACH
EUR 1698.6

Printer for Media Autoclaves
AUT4519 EACH
EUR 388.74

Bottle Pyrex Media 100ml
BOT5350 PK10
EUR 78.59

Bottle Pyrex Media 250ml
BOT5352 PK10
EUR 92.75

Hyclone ADCF-MAb Media
SH3034901 500ML
EUR 30.78

1 L SSPE, 20X, LIQUID
46-021-CM 1L/pk
EUR 242.4
Description: Media Catalog; Classical Media

SSC Buffer - 20x Liquid Concentrate
D0623 1L
EUR 74.62

TMB Liquid Substrate, for ELISA
F027-100 100 ml
EUR 262.8
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.

TMB Liquid Substrate, for ELISA
F100027 1000 ml
EUR 817.2
Description: TMB Liquid Substrate, for ELISA by Cygnus Technologies is available in Europe via Gentaur.

Phenol-liquid, saturated with water
PC5523 100ml
EUR 110.11

10mL Gentamicin Sulfate Liquid 5
30-005-CR PK10
EUR 523.26

1L TSB animal free Liquid
46-060-CM EACH
EUR 153.9

20L TSB animal free Liquid
46-060-LB EACH
EUR 1249.44

100mL-Glutamine 200 mM Liquid
25-005-CI PK6
EUR 107.16

Julabo Liquid Level Cooling Set
BAT5234 EACH
EUR 343.14

Desderman pure Liquid 500 mL
CLE3234 PK20
EUR 159.6

Cryoscope Bath Liquid Dairy Testing
CRYBL 500ML
EUR 25.08

TRITON2 PLUS Liquid Nitrogen Generator
SLS6144 EACH
EUR 38304

LN20AC Compact Liquid Nitrogen Generator
SLS6146 EACH
EUR 41497.14

Buffer Sachet Liquid pH4 20ml
PHB1391 PK25
EUR 43.09

Although there is no perfect substitute is found among alternative to FBS, the algorithm for the switch to be useful in future testing new alternative to FBS as a need to switch from FBS and expanding pharmaceutical products safety for human use.
Keywords: C2BBe1; SH-SY5Y; fetal bovine serum; neurospheres; xeno-free.