As a supplement widely used cell culture, fetal bovine serum (FBS) port of the high content of growth factors, proliferation, and adhesion. However, high cost, bio-safety, possible transmission of xenogeneic agents, restricted access, and ethical controversies major obstacle that prevents the use of these additives. Thus, new alternatives have been proposed by various pros and cons. However, caution must be taken in selecting a suitable replacement given that changes in the main aspects of cultured cells can be biased consequences of clinical applications.
Here, the authors ( Corneel Verburg) evaluated the impact of harvesting cord blood serum HES (CBS-HES), as an enriched source of growth factors, the mesenchymal stem cells basic (MSC) characteristics. In this experiment, umbilical MSCs cord-derived isolated and maintained with Dulbecco’s Modified Eagle containing either 10, 15, and 20% CBS-HES or FBSS Medium to compare their morphology, immunophenotype, growth and proliferation rate, the rate of death, cell cycle, and gene expression profiles. Despite all the media that support the expansion of MSC enriched with comparable morphology, cell surface markers, the mortality rate, c-MYC and p16 expression, and the growth rate, CBS-HES cells significantly (P <0.05) expressed more hTERT gene in concentration – a dependent manner.
However, no significant shift was observed in the cell cycle of cultured cells using the same concentration of additive, a finding that was subsequently confirmed by Ki-67 immunostaining.
CBS-HES as additives available and affordable, it seems to be optimal, it is relatively safe and promising alternative FBS for cultivation, propagation, and subsequent clinical application of MSC.
Dr. Djoumana Ounas
Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells Favorable Effects of Fetal Bovine Serum in Vitro Cryosurvival of Chicken spermatozoa
chicken spermatozoa are highly susceptible to cryopreservation often requires extender containing additives to improve the quality of post-thaw them. Although the protective properties of fetal bovine serum (FBS) for freezing cells cultured tissue is widely known, its potential as a cryoprotectant for most of the sperm cells have not been explored. Thus, the goal of our research is to (i) investigate the protective effect of FBS in different concentrations (0, 5, 10, 15 and 20%) to cryodamages in spermatozoa chicken, and (ii) to test the concentration of FBS that produces the best preservation compared 1 mg / mL of cholesterol-loaded cyclodextrins (CLC).
Samples were assessed before and after freezing on sperm motility parameters, plasma and integrities acrosome membrane, mitochondrial membrane potential, oxidative stress and peroxidation plasma membrane.
Our findings show that, despite their beneficial effects on fresh spermatozoa, the higher the concentration of FBS (15 and 20%) obtained the worst results for most motility and functional parameters after thawing. Conversely, lowering the concentration of FBS (5 and 10%) improved post-thaw all variables when compared with controls. After that, based on regression analysis, the concentration of 7% FBS have to be assessed against the CLC in the experiment was prepared by four groups: control, FBS, CLC, and FBS + CLC.
FBS and FBS + CLC group exhibited higher progressive motility in fresh samples, whereas only FBS higher retained post-thaw progressive motility. In addition, the incorporation FBS extender to increase the percentage of cells that quickly and reducing the production of free radicals and peroxidation plasma membrane. Together, these results indicate that FBS minimize some of the harmful effects of cryopreservation, providing an alternative to chicken semen extenders which seems superior in many aspects CLC at 1 mg / mL.
iFluor™ 610 succinimidyl ester
1038
AAT Bioquest
1 mg
EUR 219
iFluor™ 546 succinimidyl ester
1048
AAT Bioquest
1 mg
EUR 219
iFluor™ 568 succinimidyl ester
1049
AAT Bioquest
1 mg
EUR 219
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11001
AAT Bioquest
1 L
EUR 202
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11003
AAT Bioquest
1 L
EUR 480
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
11004
AAT Bioquest
5x10 mL
EUR 132
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11012
AAT Bioquest
100 ml
EUR 115
ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1105
AAT Bioquest
2 Labelings
EUR 176
Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*
11050
AAT Bioquest
1 g
EUR 115
Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
11100
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
11105
AAT Bioquest
500 tests
EUR 219
ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1114
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1120
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1123
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1126
AAT Bioquest
2 Labelings
EUR 50
Amplite™ Colorimetric Glucose Oxidase Assay Kit
11299
AAT Bioquest
500 Tests
EUR 306
ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1130
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
11300
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
11301
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
11302
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
11303
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
11304
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit
11305
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*
11306
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Xanthine Oxidase Assay Kit
11307
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Acetylcholinesterase Assay Kit
11400
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
11401
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
11402
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
11403
AAT Bioquest
200 Tests
EUR 219
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
11503
AAT Bioquest
200 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*
11504
AAT Bioquest
100 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*
11505
AAT Bioquest
100 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
11506
AAT Bioquest
100 Tests
EUR 263
Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11540
AAT Bioquest
1000 Tests
EUR 219
Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11541
AAT Bioquest
1000 Tests
EUR 219
Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
11551
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
11552
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*
11553
AAT Bioquest
500 Tests
EUR 306
Amplite™ Luminometric Peroxidase (HRP) Assay Kit
11559
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
11560
AAT Bioquest
200 Tests
EUR 306
FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*
11600
AAT Bioquest
5 mg
EUR 132
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11610
AAT Bioquest
25 mg
EUR 115
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11612
AAT Bioquest
10 g
EUR 898
MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11614
AAT Bioquest
25 mg
EUR 115
MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11617
AAT Bioquest
5 g
EUR 480
pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*
11619
AAT Bioquest
25 mg
EUR 115
CF-MUP, sodium salt *Superior alternative to MUP*
11628
AAT Bioquest
10 mg
EUR 50
Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
11950
AAT Bioquest
500 Tests
EUR 176
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*
11952
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
11953
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*
11954
AAT Bioquest
500 Tests
EUR 219
Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
11956
AAT Bioquest
100 Tests
EUR 219
ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1227
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1235
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1240
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1245
AAT Bioquest
2 Labelings
EUR 176
Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12481
AAT Bioquest
25 mg
EUR 219
Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12482
AAT Bioquest
100 mg
EUR 480
ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1250
AAT Bioquest
2 Labelings
EUR 176
D-Luciferin, free acid *CAS#: 2591-17-5*
12501
AAT Bioquest
25 mg
EUR 115
D-Luciferin, free acid *CAS#: 2591-17-5*
12502
AAT Bioquest
100 mg
EUR 132
D-Luciferin, free acid *CAS#: 2591-17-5*
12503
AAT Bioquest
1 g
EUR 528
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12505
AAT Bioquest
25 mg
EUR 115
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12506
AAT Bioquest
100 mg
EUR 132
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12507
AAT Bioquest
1 g
EUR 263
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12509
AAT Bioquest
25 mg
EUR 115
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12510
AAT Bioquest
100 mg
EUR 132
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12511
AAT Bioquest
1 g
EUR 528
D-Luciferin phosphate *CAS 145613-12-3*
12512
AAT Bioquest
1 mg
EUR 219
D-Luciferin galactoside *CAS 131474-38-9*
12513
AAT Bioquest
5 mg
EUR 219
D-Luciferin methyl ester *CAS 73918-26-0*
12514
AAT Bioquest
5 mg
EUR 219
D-Luciferin ethyl ester *CAS 135251-85-3*
12515
AAT Bioquest
5 mg
EUR 219
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12518
AAT Bioquest
1 plate
EUR 176
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12519
AAT Bioquest
10 plates
EUR 393
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12520
AAT Bioquest
100 plates
EUR 2616
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530
AAT Bioquest
1 plate
EUR 219
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531
AAT Bioquest
10 Plates
EUR 702
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532
AAT Bioquest
100 plates
EUR 3486
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535
AAT Bioquest
1 plate
EUR 219
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536
AAT Bioquest
10 plates
EUR 876
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537
AAT Bioquest
100 plates
EUR 3486
ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1255
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit
12551
AAT Bioquest
200 Tests
EUR 393
BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12560
AAT Bioquest
1 mg
EUR 219
BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12561
AAT Bioquest
5 mg
EUR 393
ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1260
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
12601
AAT Bioquest
500 Tests
EUR 306
Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
12602
AAT Bioquest
200 Tests
EUR 306
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*
12603
AAT Bioquest
200 Tests
EUR 306
Amplite™ Colorimetric Beta-Galactosidase Assay Kit
12604
AAT Bioquest
200 Tests
EUR 306
HIS Lite™ Cy3 Bis NTA-Ni Complex
12610
AAT Bioquest
1 mg
EUR 393
HIS Lite™ Cy5 Bis NTA-Ni Complex
12613
AAT Bioquest
1 mg
EUR 393
ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1265
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1290
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1292
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1294
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1299
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
1300
AAT Bioquest
2 Labelings
EUR 876
Benzoyl-Leu-Gly-Arg-Aminoluciferin
13000
AAT Bioquest
1 mg
EUR 480
BOC-Leu-Gly-Arg-Aminoluciferin
13001
AAT Bioquest
1 mg
EUR 480
BOC-Val-Pro-Arg-Aminoluciferin
13002
AAT Bioquest
1 mg
EUR 480
D-VLK-AMC [D-Val-Leu-Lys-AMC]
13201
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-AFC *CAS 201608-14-2*
13401
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-AMC *CAS 169332-61-0*
13402
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-CHO *CAS 169332-60-9*
13403
AAT Bioquest
1 mg
EUR 132
Ac-DEVD-pNA *CAS 189950-66-1*
13405
AAT Bioquest
5 mg
EUR 132
Ac-IETD-AFC *CAS 211990-57-7*
13410
AAT Bioquest
5 mg
EUR 219
Ac-IETD-CHO *CAS 191338-86-0*
13412
AAT Bioquest
5 mg
EUR 219
Z-IETD-pNA *CAS 219138-21-3*
13413
AAT Bioquest
5 mg
EUR 219
Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*
1342
AAT Bioquest
2 Labelings
EUR 306
Z-IETD-AFC *CAS 219138-02-0*
13425
AAT Bioquest
5 mg
EUR 158
