NatTrol Clostridium difficile

Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

As a supplement widely used cell culture, fetal bovine serum (FBS) port of the high content of growth factors, proliferation, and adhesion. However, high cost, bio-safety, possible transmission of xenogeneic agents, restricted access, and ethical controversies major obstacle that prevents the use of these additives. Thus, new alternatives have been proposed by various pros and cons. However, caution must be taken in selecting a suitable replacement given that changes in the main aspects of cultured cells can be biased consequences of clinical applications.

Here, the authors ( Corneel Verburg) evaluated the impact of harvesting cord blood serum HES (CBS-HES), as an enriched source of growth factors, the mesenchymal stem cells basic (MSC) characteristics. In this experiment, umbilical MSCs cord-derived isolated and maintained with Dulbecco’s Modified Eagle containing either 10, 15, and 20% CBS-HES or FBSS Medium to compare their morphology, immunophenotype, growth and proliferation rate, the rate of death, cell cycle, and gene expression profiles. Despite all the media that support the expansion of MSC enriched with comparable morphology, cell surface markers, the mortality rate, c-MYC and p16 expression, and the growth rate, CBS-HES cells significantly (P <0.05) expressed more hTERT gene in concentration – a dependent manner.

However, no significant shift was observed in the cell cycle of cultured cells using the same concentration of additive, a finding that was subsequently confirmed by Ki-67 immunostaining.

CBS-HES as additives available and affordable, it seems to be optimal, it is relatively safe and promising alternative FBS for cultivation, propagation, and subsequent clinical application of MSC.

Dr. Djoumana Ounas


Keywords: alternative FBS; Fetal bovine serum; Hydroxyethyl starch; Mesenchymal stem cells; Umbilical cord.

Cord blood serum teste par NatTrol de C. Difficile
Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

Favorable Effects of Fetal Bovine Serum in Vitro Cryosurvival of Chicken spermatozoa

chicken spermatozoa are highly susceptible to cryopreservation often requires extender containing additives to improve the quality of post-thaw them. Although the protective properties of fetal bovine serum (FBS) for freezing cells cultured tissue is widely known, its potential as a cryoprotectant for most of the sperm cells have not been explored. Thus, the goal of our research is to (i) investigate the protective effect of FBS in different concentrations (0, 5, 10, 15 and 20%) to cryodamages in spermatozoa chicken, and (ii) to test the concentration of FBS that produces the best preservation compared 1 mg / mL of cholesterol-loaded cyclodextrins (CLC).

Samples were assessed before and after freezing on sperm motility parameters, plasma and integrities acrosome membrane, mitochondrial membrane potential, oxidative stress and peroxidation plasma membrane.

Our findings show that, despite their beneficial effects on fresh spermatozoa, the higher the concentration of FBS (15 and 20%) obtained the worst results for most motility and functional parameters after thawing. Conversely, lowering the concentration of FBS (5 and 10%) improved post-thaw all variables when compared with controls. After that, based on regression analysis, the concentration of 7% FBS have to be assessed against the CLC in the experiment was prepared by four groups: control, FBS, CLC, and FBS + CLC.

FBS and FBS + CLC group exhibited higher progressive motility in fresh samples, whereas only FBS higher retained post-thaw progressive motility. In addition, the incorporation FBS extender to increase the percentage of cells that quickly and reducing the production of free radicals and peroxidation plasma membrane. Together, these results indicate that FBS minimize some of the harmful effects of cryopreservation, providing an alternative to chicken semen extenders which seems superior in many aspects CLC at 1 mg / mL.

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AZIDE DEXTROSE BROTH

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ANAEROBIC AGAR

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AGAROSE 25G-125G -500G

A01-118-500g 500 g
EUR 494

IL-2 Receptor ?, Human Recombinant

7100-10
EUR 196

IL-2 Receptor ?, Human Recombinant

7100-50
EUR 637

Human CellExp? IL-4 R?, Human Recombinant

7101-10
EUR 229

Human CellExp? IL-4 R?, Human Recombinant

7101-50
EUR 881

Human CellExp? IL-6 R?, Human Recombinant

7102-10
EUR 207

Human CellExp? IL-6 R?, Human Recombinant

7102-50
EUR 800

IL-9, Rat Recombinant

7103-10
EUR 278

IL-9, Rat Recombinant

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EUR 1132

IL-12p80, Human Recombinant

7104-10
EUR 392

IL-12p80, Human Recombinant

7104-50
EUR 1675

IL-17A, Mouse Recombinant

7105-10
EUR 196

IL-17A, Mouse Recombinant

7105-50
EUR 675

IL-17B, Human Recombinant

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IL-17B, Human Recombinant

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EUR 675

IL-17D, Human Recombinant

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IL-17D, Human Recombinant

7107-50
EUR 675

Human CellExp? IL-34, Human Recombinant

7108-10
EUR 278

Human CellExp? IL-34, Human Recombinant

7108-50
EUR 1132

IL-36RA, Human Recombinant

7109-10
EUR 196

IL-36RA, Human Recombinant

7109-50
EUR 675

IL-36?, Human Recombinant

7110-10
EUR 278

IL-36?, Human Recombinant

7110-50
EUR 1132

IL-36?, Human Recombinant

7111-10
EUR 278

IL-36?, Human Recombinant

7111-50
EUR 1132

Activin B, Human Recombinant

7112-10
EUR 479

Activin B, Human Recombinant

7112-50
EUR 2094

AITRL, Human Recombinant

7113-10
EUR 251

AITRL, Human Recombinant

7113-50
EUR 936

Amphiregulin, Human Recombinant

7114-10
EUR 147

Amphiregulin, Human Recombinant

7114-50
EUR 376

ANG-1, Human Recombinant

7115-10
EUR 251

ANG-1, Human Recombinant

7115-50
EUR 936

ANG-2, Human Recombinant

7116-10
EUR 251

ANG-2, Human Recombinant

7116-50
EUR 936

APRIL, Human Recombinant

7117-10
EUR 311

APRIL, Human Recombinant

7117-50
EUR 1300

APRIL, Mouse Recombinant

7118-10
EUR 196

APRIL, Mouse Recombinant

7118-50
EUR 675

Betacellulin, Murine Recombinant

7119-10
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Betacellulin, Murine Recombinant

7119-50
EUR 675

Cardiotrophin-1, Murine Recombinant

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EUR 278

Cardiotrophin-1, Murine Recombinant

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EUR 1132

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7126-10
EUR 251

sCD100/Semaphorin-4D, Human Recombinant

7126-50
EUR 936

CTGFL/WISP-2, Human Recombinant

7129-10
EUR 234

CTGFL/WISP-2, Human Recombinant

7129-50
EUR 860

Human CellExp? DKK-1, Human Recombinant

7132-10
EUR 278

Human CellExp? DKK-1, Human Recombinant

7132-50
EUR 1132

Human CellExp? sDLL-1, Human Recombinant

7133-10
EUR 207

Human CellExp? sDLL-1, Human Recombinant

7133-50
EUR 675

Human CellExp? sDLL-4, Human Recombinant

7134-10
EUR 207

Human CellExp? sDLL-4, Human Recombinant

7134-50
EUR 675

Eotaxin-2/CCL24, murine recombinant

7137-10
EUR 207

Eotaxin-2/CCL24, murine recombinant

7137-50
EUR 675

Eotaxin-3/CCL26, human recombinant

7138-10
EUR 207

Eotaxin-3/CCL26, human recombinant

7138-50
EUR 675

Human CellExp? Fetuin A/ASHG, Human Recombinant

7142-10
EUR 153

Human CellExp? Fetuin A/ASHG, Human Recombinant

7142-50
EUR 381

Histone H3 Peptide, biotin conjugate

7143-01
EUR 240

Histone H3 Peptide, biotin conjugate

7143-1
EUR 1132

Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-01
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Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-1
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FGF-5, human recombinant

7147-10
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FGF-5, human recombinant

7147-50
EUR 359

FGF-6, human recombinant

7148-10
EUR 207

FGF-6, human recombinant

7148-50
EUR 675

FGF-16, human recombinant

7149-10
EUR 207

FGF-16, human recombinant

7149-50
EUR 675

FGF-17, human recombinant

7150-10
EUR 207

FGF-17, human recombinant

7150-50
EUR 675

FGF-23, human recombinant

7151-10
EUR 229

FGF-23, human recombinant

7151-50
EUR 860

GASP-1, human recombinant

7152-10
EUR 229

GASP-1, human recombinant

7152-50
EUR 833

GCP-2 (CXCL6), human recombinant

7153-10
EUR 207

GCP-2 (CXCL6), human recombinant

7153-50
EUR 675

BMP-9/GDF-2, human recombinant

7154-10
EUR 278

BMP-9/GDF-2, human recombinant

7154-50
EUR 1132

BMP-11/GDF-11, human recombinant

7155-10
EUR 240

BMP-11/GDF-11, human recombinant

7155-50
EUR 860

Gremlin-1, human recombinant

7159-10
EUR 142

Gremlin-1, human recombinant

7159-50
EUR 359

ICAM-1, human recombinant

7161-10
EUR 142

ICAM-1, human recombinant

7161-50
EUR 359

IFN-?1, human recombinant

7162-10
EUR 207

IFN-?1, human recombinant

7162-50
EUR 735

IFN-?2, human recombinant

7163-10
EUR 207

IFN-?2, human recombinant

7163-50
EUR 675

IGF-BP2, human recombinant

7165-10
EUR 251

IGF-BP2, human recombinant

7165-50
EUR 936

IGF-BP4, human recombinant

7166-10
EUR 251

IGF-BP4, human recombinant

7166-50
EUR 936

IGF-BP7, human recombinant

7167-10
EUR 207

IGF-BP7, human recombinant

7167-50
EUR 675

MCP-5/CCL12, murine recombinant

7168-10
EUR 207

MCP-5/CCL12, murine recombinant

7168-50
EUR 675

MIP-1?/CCL3, human recombinant

7173-10
EUR 207

MIP-1?/CCL3, human recombinant

7173-50
EUR 675

MIP-1?/CCL9/10, murine recombinant

7174-10
EUR 207

MIP-1?/CCL9/10, murine recombinant

7174-50
EUR 675

MIP-3/CCL23, human recombinant

7175-10
EUR 207

MIP-3/CCL23, human recombinant

7175-50
EUR 675

Nesfatin-1, human recombinant

7178-10
EUR 137


Keywords: Birds; poultry; reproductive technologies; Semen analysis; Frozen sperm.

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