NatTrol Clostridium difficile

Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

As a supplement widely used cell culture, fetal bovine serum (FBS) port of the high content of growth factors, proliferation, and adhesion. However, high cost, bio-safety, possible transmission of xenogeneic agents, restricted access, and ethical controversies major obstacle that prevents the use of these additives. Thus, new alternatives have been proposed by various pros and cons. However, caution must be taken in selecting a suitable replacement given that changes in the main aspects of cultured cells can be biased consequences of clinical applications.

Here, the authors ( Corneel Verburg) evaluated the impact of harvesting cord blood serum HES (CBS-HES), as an enriched source of growth factors, the mesenchymal stem cells basic (MSC) characteristics. In this experiment, umbilical MSCs cord-derived isolated and maintained with Dulbecco’s Modified Eagle containing either 10, 15, and 20% CBS-HES or FBSS Medium to compare their morphology, immunophenotype, growth and proliferation rate, the rate of death, cell cycle, and gene expression profiles. Despite all the media that support the expansion of MSC enriched with comparable morphology, cell surface markers, the mortality rate, c-MYC and p16 expression, and the growth rate, CBS-HES cells significantly (P <0.05) expressed more hTERT gene in concentration – a dependent manner.

However, no significant shift was observed in the cell cycle of cultured cells using the same concentration of additive, a finding that was subsequently confirmed by Ki-67 immunostaining.

CBS-HES as additives available and affordable, it seems to be optimal, it is relatively safe and promising alternative FBS for cultivation, propagation, and subsequent clinical application of MSC.

Dr. Djoumana Ounas


Keywords: alternative FBS; Fetal bovine serum; Hydroxyethyl starch; Mesenchymal stem cells; Umbilical cord.

Cord blood serum teste par NatTrol de C. Difficile
Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

Favorable Effects of Fetal Bovine Serum in Vitro Cryosurvival of Chicken spermatozoa

chicken spermatozoa are highly susceptible to cryopreservation often requires extender containing additives to improve the quality of post-thaw them. Although the protective properties of fetal bovine serum (FBS) for freezing cells cultured tissue is widely known, its potential as a cryoprotectant for most of the sperm cells have not been explored. Thus, the goal of our research is to (i) investigate the protective effect of FBS in different concentrations (0, 5, 10, 15 and 20%) to cryodamages in spermatozoa chicken, and (ii) to test the concentration of FBS that produces the best preservation compared 1 mg / mL of cholesterol-loaded cyclodextrins (CLC).

Samples were assessed before and after freezing on sperm motility parameters, plasma and integrities acrosome membrane, mitochondrial membrane potential, oxidative stress and peroxidation plasma membrane.

Our findings show that, despite their beneficial effects on fresh spermatozoa, the higher the concentration of FBS (15 and 20%) obtained the worst results for most motility and functional parameters after thawing. Conversely, lowering the concentration of FBS (5 and 10%) improved post-thaw all variables when compared with controls. After that, based on regression analysis, the concentration of 7% FBS have to be assessed against the CLC in the experiment was prepared by four groups: control, FBS, CLC, and FBS + CLC.

FBS and FBS + CLC group exhibited higher progressive motility in fresh samples, whereas only FBS higher retained post-thaw progressive motility. In addition, the incorporation FBS extender to increase the percentage of cells that quickly and reducing the production of free radicals and peroxidation plasma membrane. Together, these results indicate that FBS minimize some of the harmful effects of cryopreservation, providing an alternative to chicken semen extenders which seems superior in many aspects CLC at 1 mg / mL.

Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested

P761-10 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested

P761S - Ask for price

Rat mesenchymal Stem cell Flow Cytometry Kit

RAXMX-09011 10 tests
EUR 1079

Human mesenchymal Stem cell Flow Cytometry Kit

HUXMX-09011 10 tests
EUR 1359

Mouse mesenchymal Stem cell Flow Cytometry Kit

MUXMX-09011 10 tests
EUR 799

Mesenchymal Stem Cells - Adipose Derived

SC00AD1 500,000 Cells
EUR 1086

MSCGro-Humanized Mesenchymal Stem Cell, complete

SC00B2-500 500 ml
EUR 450

Mesenchymal Stem Cells - Placental Derived

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Human Bone Marrow Mesenchymal Stem Cells

HUXMA-01001 1*10^6/vial
EUR 1030

Canine Bone Marrow Mesenchymal Stem Cells

CAXMX-01001 1*10^6/vial
EUR 999

Rabbit Bone Marrow Mesenchymal Stem Cells

RBXMX-01001 1*10^6/vial
EUR 649

Mesenchymal Stem Cells - Bone Marrow Derived

SC00BM1 500,000 cells
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SD Rat Bone Marrow Mesenchymal Stem Cells

RASMX-01001 1*10^6/vial
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Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-01001 1*10^6/vial
EUR 1159

F344 Rat Bone Marrow Mesenchymal Stem Cells

RAFMX-01001 1*10^6/vial
EUR 479

Human Adipose-derived Mesenchymal Stem Cells

HUXMD-01001 1*10^6/vial
EUR 1079

Wistar Rat Bone Marrow Mesenchymal Stem Cells

RAWMX-01001 1*10^6/vial
EUR 479

Canine Adipose-derived Mesenchymal Stem Cells

CAXMD-01001 1*10^6/vial
EUR 1200

Rabbit Adipose-derived Mesenchymal Stem Cells

RBXMD-01001 1*10^6/vial
EUR 799

Human Mesenchymal Stem Cells Expressing Oct3/4

SC00A6 500000
EUR 1420.8

SD Rat Adipose-derived Mesenchymal Stem Cells

RASMD-01001 1*10^6/vial
EUR 639

Human Mesenchymal Stem Cells - Bone Marrow Derived

MSC001 500,000+ Cells
EUR 1545.6

Rat Bone Marrow Mesenchymal Stem Cells With GFP

RBXMX-01101 1*10^6/vial
EUR 1089

Rat Bone Marrow Mesenchymal Stem Cells With RFP

RBXMX-01201 1*10^6/vial
EUR 1089

Human Umbilical Cord Blood Mesenchymal Stem Cells

HUXUB-01001 1*10^6/vial
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Balb/c Mouse Bone Marrow Mesenchymal Stem Cells

MUCMX-01001 1*10^6/vial
EUR 639

Human Bone Marrow Mesenchymal Stem Cells With GFP

HUXMA-01101 1*10^6/vial
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Human Bone Marrow Mesenchymal Stem Cells With RFP

HUXMA-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells

MUBMX-01001 1*10^6/vial
EUR 639

Wistar Rat Adipose-derived Mesenchymal Stem Cells

RAWMD-01001 1*10^6/vial
EUR 639

Canine Bone Marrow Mesenchymal Stem Cells With GFP

CAXMX-01101 1*10^6/vial
EUR 1229

Canine Bone Marrow Mesenchymal Stem Cells With RFP

CAXMX-01201 1*10^6/vial
EUR 1229

SD Rat Bone Marrow Mesenchymal Stem Cells With GFP

RASMX-01101 1*10^6/vial
EUR 929

SD Rat Bone Marrow Mesenchymal Stem Cells With RFP

RASMX-01201 1*10^6/vial
EUR 929

Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7

T0352 1x106 cells / 1.0 ml Ask for price

Human Umbilical Cord Mesenchymal Stem Cells With GFP

HUXUC-01101 1*10^6/vial
EUR 1599

F344 Rat Bone Marrow Mesenchymal Stem Cells With GFP

RAFMX-01101 1*10^6/vial
EUR 929

F344 Rat Bone Marrow Mesenchymal Stem Cells With RFP

RAFMX-01201 1*10^6/vial
EUR 929

Balb/c Mouse Adipose-derived Mesenchymal Stem Cells

MUCMD-01001 1*10^6/vial
EUR 649

MSCGro-Humanized Mesenchymal Stem Cell Clinical Grade Medium, SF

SC00B4-500 500 ml
EUR 434.4

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled

SC00A3 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc

T0169 1x106 cells / 1.0 ml Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras

T0170 1x106 cells / 1.0 ml Ask for price

Wistar Rat Bone Marrow Mesenchymal Stem Cells With GFP

RAWMX-01101 1*10^6/vial
EUR 929

Wistar Rat Bone Marrow Mesenchymal Stem Cells With RFP

RAWMX-01201 1*10^6/vial
EUR 929

Human Adipose-derived Mesenchymal Stem Cells With GFP

HUXMD-01101 1*10^6/vial
EUR 1479

Human Adipose-derived Mesenchymal Stem Cells With RFP

HUXMD-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Adipose-Derived Mesenchymal Stem Cells

MUBMD-01001 1*10^6/vial
EUR 649

Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40

T0167 1x106 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells-Green Fluorescent-Labeled

SC00A2 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT

T0168 1x106 cells / 1.0 ml Ask for price

SD Rat Adipose-derived Mesenchymal Stem Cells With GFP

RASMD-01101 1*10^6/vial
EUR 1239

SD Rat Adipose-derived Mesenchymal Stem Cells With RFP

RASMD-01201 1*10^6/vial
EUR 1239

Immortalized Human Bone Marrow Mesenchymal Stem Cells SV40

T0520 1x106 cells / 1.0 ml Ask for price

Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP

MUCMX-01101 1*10^6/vial
EUR 1079

Human Umbilical Cord Blood Mesenchymal Stem Cells With GFP

HUXUB-01101 1*10^6/vial
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Complete Medium For Rat Bone Marrow Mesenchymal Stem Cells

RAXMX-90011 500mL
EUR 289

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP

MUBMX-01101 1*10^6/vial
EUR 1079

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With RFP

MUBMX-01201 1*10^6/vial
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Complete Medium For Human Bone Marrow Mesenchymal Stem Cells

HUXMA-90011 500mL
EUR 319

Complete Medium For Mouse Bone Marrow Mesenchymal Stem Cells

MUXMX-90011 500mL
EUR 289

Complete Medium For Canine Bone Marrow Mesenchymal Stem Cells

CAXMX-90011 500mL
EUR 319

Complete Medium For Monkey Bone Marrow Mesenchymal Stem Cells

MKXMA-90011 500mL
EUR 289

Complete Medium For Rabbit Bone Marrow Mesenchymal Stem Cells

RBXMX-90011 500mL
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Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90011 500mL
EUR 319

Hyclone Human Mesenchymal Stem Cell Screened FBS US Origin 500 mL (SH30070.03M)

HYC31 EACH
EUR 1156.76

Complete Medium For Rat Adipose-derived Mesenchymal Stem Cells

RAXMD-90011 500mL
EUR 289

C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP

MUBMD-01101 1*10^6/vial
EUR 1239

Complete Medium For Canine Adipose-derived Mesenchymal Stem Cells

CAXMD-90011 500mL
EUR 289

Complete Medium For Human Adipose-derived Mesenchymal Stem Cells

HUXMD-90011 500mL
EUR 319

Complete Medium For Monkey Adipose-derived Mesenchymal Stem Cells

MKXMD-90011 500mL
EUR 289

Complete Medium For Mouse Adipose-derived Mesenchymal Stem Cells

MUXMD-90011 500mL
EUR 289

Complete Medium For Rabbit Adipose-derived Mesenchymal Stem Cells

RBXMD-90011 500mL
EUR 289

Complete Medium For Human Umbilical Cord Blood Mesenchymal Stem Cells

HUXUB-90011 500mL
EUR 319

Osteogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells

RAXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells

RAXMX-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells

HUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells

HUXMX-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells

MUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells

MUXMX-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells

RAXMX-90041 100mL
EUR 409

Chondrogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells

HUXMD-90041 100mL
EUR 409

Hepatogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells

HUXMX-90101 50+100+200mL
EUR 759

Chondrogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells

MUXMX-90041 100mL
EUR 409

PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes

EXOP-140A-1 50 ug
EUR 525

Mesenchymal Cell Kit

abx290006-50test 50 test
EUR 2949.6

Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells

RAXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells

RAXMD-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells

HUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells

HUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-90041 100mL
EUR 409

Osteogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells

MUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells

MUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells

RAXMD-90041 100mL
EUR 409

PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes

EXOP-145A-1 50 ug
EUR 525

Osteogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)

GUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)

GUXMX-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)

GUXMX-90041 100mL
EUR 409

Chondrogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells

MUXMD-90041 100mL
EUR 409

Osteogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)

GUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)

GUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)

GUXMD-90041 100mL
EUR 409

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested

CA068-010 100g
EUR 375.6

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested

CA068-050 500g
EUR 1170

HQ Fetal Bovine Serum

abx098128-500ml 500 ml
EUR 895.2

EQ Fetal Bovine Serum

abx298025-500ml 500 ml
EUR 1362

FBS - Fetal Bovine Serum

FBS001 500 ml
EUR 654

Vimentin (Mesenchymal Cell Marker) (VM452) Antibody

BNUB0452-50 50uL
EUR 486
Description: Primary antibody against Vimentin (Mesenchymal Cell Marker) (VM452), 1mg/mL

Fetal Bovine Serum, Triple Low

F0610-050 500ml Ask for price

GOOD fetal bovine serum - 500 mL

DDP40-37500 EACH
EUR 144.78

Fetal Bovine Serum(Best-Quality )

FBSAD-01011 100/500mL Ask for price

Fetal Bovine Serum (FBS) [50 ml]

ABP-MED-FBS0050 50ml Ask for price

Fetal Bovine Serum (FBS) [500 ml]

ABP-MED-FBS0500 500ml Ask for price

Fetal Bovine Serum(Without Exosome )

FBSNE-01061 20/50mL Ask for price

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)

RA0338-C.1 0.1 ml
EUR 150

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)

RA0338-C.5 0.5 ml
EUR 296.4

Vimentin (Mesenchymal Cell Marker); Clone VM452 (Concentrate)

RA0337-C.1 0.1 ml
EUR 150

Vimentin (Mesenchymal Cell Marker); Clone VM452 (Concentrate)

RA0337-C.5 0.5 ml
EUR 360

Fetal Bovine Serum(Superior-Quality)

FBSSR-01021 500mL
EUR 939

Fetal Bovine Serum(Standard-Quality)

FBSST-01033 500mL
EUR 599

Fetal Bovine Serum, Premium, US Origin

F0600-050 500ml
EUR 789.6

Monoclonal Vimentin (Mesenchymal Cell Marker) Antibody, Clone: VM452

AMM01396G 7 ml
EUR 580.8
Description: A Monoclonal antibody against Human Vimentin (Mesenchymal Cell Marker). The antibodies are raised in Mouse and are from clone VM452. This antibody is applicable in WB, IHC and IF, FC

Fetal Bovine Serum, Hormone Level Reduced

F0620-050 500ml
EUR 902.4

Monoclonal Vimentin (Mesenchymal Cell Marker) Antibody, Clone: SPM576

AMM01397G 7 ml
EUR 580.8
Description: A Monoclonal antibody against Human Vimentin (Mesenchymal Cell Marker). The antibodies are raised in Mouse and are from clone SPM576. This antibody is applicable in WB, IHC and IF, FC

Monoclonal Vimentin (Mesenchymal Cell Marker) Antibody, Clone: VM1170

AMM01401G 7 ml
EUR 580.8
Description: A Monoclonal antibody against Human Vimentin (Mesenchymal Cell Marker). The antibodies are raised in Mouse and are from clone VM1170. This antibody is applicable in WB, IHC and IF, FC

Monoclonal Vimentin (Mesenchymal Cell Marker) Antibody, Clone: LN-6

AMM01398G 7 ml
EUR 580.8
Description: A Monoclonal antibody against Human Vimentin (Mesenchymal Cell Marker). The antibodies are raised in Mouse and are from clone LN-6. This antibody is applicable in WB and IHC-P, IF

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1388.4

Fetal Bovine Serum,Low IgG , < 5 ug/ml

F0710-050 500 ml
EUR 1437.6

500 ML FETAL BOVINE SERUM, PREMIUM (DIALYZED)

35-071-CV 500 mL/pk
EUR 1110
Description: Serum; Serum

Immortalized Human Bone Marrow Mesenchymal Cells - hTERT

T0523 1x106 cells / 1.0 ml Ask for price

500 ML FETAL BOVINE SERUM, AUSTRALIAN ORIGIN

35-076-CV 500 mL/pk
EUR 952.8
Description: Serum; Serum

Fetal Bovine Serum (FBS) proteins for ELISA

FBS15-N-1 1 ml
EUR 270

500 ML FETAL BOVINE SERUM, PREMIUM (LOW IGG)

35-073-CV 500 mL/pk
EUR 2173.2
Description: Serum; Serum

FBS-HI - Heat Inactivated Fetal Bovine Serum

FBS001-HI 500 ml
EUR 676.8

Fetal Bovine Serum,IgG Depleted, < 2 ug/ml

F0720-050 500 ml
EUR 1437.6

Fetal Bovine Serum, Premium, US Origin, Dialyzed

F0602-050 500ml
EUR 1275.6

Fetal Bovine Serum (FBS), Premium, 100% US Origin

P760-1 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin

P760-10 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin

P760S - Ask for price

Immortalized mouse adipose-derived mesenchymal cells (iMADs)

T0541 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM)

T0622 1x106 cells / 1.0 ml Ask for price

Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1)

T0687 1x106 cells / 1.0 ml Ask for price

Rabbit Anti-Fetal Bovine Serum (FBS) protein IgG-Agarose affinity matrix to remove fetal bovine serum

FBS12-AS 2 ml
EUR 562.8

Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2)

T0653 1x106 cells / 1.0 ml Ask for price


Keywords: Birds; poultry; reproductive technologies; Semen analysis; Frozen sperm.

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