Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

As a supplement widely used cell culture, fetal bovine serum (FBS) port of the high content of growth factors, proliferation, and adhesion. However, high cost, bio-safety, possible transmission of xenogeneic agents, restricted access, and ethical controversies major obstacle that prevents the use of these additives. Thus, new alternatives have been proposed by various pros and cons. However, caution must be taken in selecting a suitable replacement given that changes in the main aspects of cultured cells can be biased consequences of clinical applications.

Here, the authors ( Corneel Verburg) evaluated the impact of harvesting cord blood serum HES (CBS-HES), as an enriched source of growth factors, the mesenchymal stem cells basic (MSC) characteristics. In this experiment, umbilical MSCs cord-derived isolated and maintained with Dulbecco’s Modified Eagle containing either 10, 15, and 20% CBS-HES or FBSS Medium to compare their morphology, immunophenotype, growth and proliferation rate, the rate of death, cell cycle, and gene expression profiles. Despite all the media that support the expansion of MSC enriched with comparable morphology, cell surface markers, the mortality rate, c-MYC and p16 expression, and the growth rate, CBS-HES cells significantly (P <0.05) expressed more hTERT gene in concentration – a dependent manner.

However, no significant shift was observed in the cell cycle of cultured cells using the same concentration of additive, a finding that was subsequently confirmed by Ki-67 immunostaining.

CBS-HES as additives available and affordable, it seems to be optimal, it is relatively safe and promising alternative FBS for cultivation, propagation, and subsequent clinical application of MSC.

Dr. Djoumana Ounas

Keywords: alternative FBS; Fetal bovine serum; Hydroxyethyl starch; Mesenchymal stem cells; Umbilical cord.

Favorable Effects of Fetal Bovine Serum in Vitro Cryosurvival of Chicken spermatozoa

chicken spermatozoa are highly susceptible to cryopreservation often requires extender containing additives to improve the quality of post-thaw them. Although the protective properties of fetal bovine serum (FBS) for freezing cells cultured tissue is widely known, its potential as a cryoprotectant for most of the sperm cells have not been explored. Thus, the goal of our research is to (i) investigate the protective effect of FBS in different concentrations (0, 5, 10, 15 and 20%) to cryodamages in spermatozoa chicken, and (ii) to test the concentration of FBS that produces the best preservation compared 1 mg / mL of cholesterol-loaded cyclodextrins (CLC).

Samples were assessed before and after freezing on sperm motility parameters, plasma and integrities acrosome membrane, mitochondrial membrane potential, oxidative stress and peroxidation plasma membrane.

Our findings show that, despite their beneficial effects on fresh spermatozoa, the higher the concentration of FBS (15 and 20%) obtained the worst results for most motility and functional parameters after thawing. Conversely, lowering the concentration of FBS (5 and 10%) improved post-thaw all variables when compared with controls. After that, based on regression analysis, the concentration of 7% FBS have to be assessed against the CLC in the experiment was prepared by four groups: control, FBS, CLC, and FBS + CLC.

FBS and FBS + CLC group exhibited higher progressive motility in fresh samples, whereas only FBS higher retained post-thaw progressive motility. In addition, the incorporation FBS extender to increase the percentage of cells that quickly and reducing the production of free radicals and peroxidation plasma membrane. Together, these results indicate that FBS minimize some of the harmful effects of cryopreservation, providing an alternative to chicken semen extenders which seems superior in many aspects CLC at 1 mg / mL.

CF568 donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20105	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF568 donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20106	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF568 rabbit anti-goat IgG(H+L)
9-20107	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF568 goat anti-guinea pig IgG(H+L)
9-20108	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-mouse IgG(H+L)
9-20110	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20111	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-rabbit IgG(H+L)
9-20112	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20113	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20114	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20115	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20116	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-goat IgG(H+L)
9-20117	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-guinea pig IgG(H+L)
9-20118	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-mouse IgG(H+L)
9-20120	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20121	Biotium	EUR 319.20 EUR 326.40	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-rabbit IgG(H+L)
9-20122	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20123	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20124	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-rabbit IgG(H+L), highly cross-adsorbed
9-20125	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20126	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20127	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 rabbit anti-goat IgG(H+L)
9-20128	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-guinea pig IgG(H+L)
9-20129	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-human IgG(H+L), highly cross-adsorbed
9-20132	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20133	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20134	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 rabbit anti-rat IgG(H+L), highly cross-adsorbed
9-20135	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20136	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-rat IgG(H+L), highly cross-adsorbed
9-20137	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-swine IgG(H+L)
9-20138	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-mouse IgG(H+L)
9-20140	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-rabbit IgG(H+L)
9-20141	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20142	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20143	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20144	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20147	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20148	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20149	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-rabbit IgG(H+L), highly cross-adsorbed
9-20152	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-human IgG(H+L), highly cross-adsorbed
9-20154	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20155	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20156	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-rat IgG(H+L), highly cross-adsorbed
9-20157	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20158	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-rat IgG(H+L), highly cross-adsorbed
9-20159	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-swine IgG(H+L)
9-20160	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-chicken IgY ( IgG) (H+L)
9-20164	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 rabbit anti-chicken IgY (IgG) (H+L)
9-20165	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20166	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20167	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20168	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A donkey anti-guinea pig IgG(H+L), highly cross-adsorbed
9-20169	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 donkey anti-guinea pig IgG(H+L), highly cross-adsorbed
9-20170	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 donkey anti-guinea pig IgG(H+L), highly cross-adsorbed
9-20171	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A rabbit anti-sheep IgG(H+L), highly cross-adsorbed
9-20172	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-sheep IgG(H+L), highly cross-adsorbed
9-20173	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 rabbit anti-sheep IgG(H+L), highly cross-adsorbed
9-20174	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20175	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20176	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20177	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R donkey anti-rabbit IgG(H+L), highly cross-adsorbed
9-20178	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20179	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF405M goat anti-mouse IgG(H+L)
9-20180	Biotium	EUR 271.20 EUR 277.20	0.5 mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF405M goat anti-rabbit IgG(H+L)
9-20181	Biotium	EUR 271.20 EUR 277.20	0.5 mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF405M goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20182	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin goat anti-mouse IgG(H+L)
9-20183	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20184	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin goat anti-rabbit IgG(H+L)
9-20185	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20186	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20189	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20190	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin donkey anti-rabbit IgG(H+L), highly cross-adsorbed
9-20191	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R goat anti-mouse IgG(H+L)
9-20197	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-guinea pig IgG(H+L)
9-20198	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R donkey anti-rat IgG(H+L), highly cross-adsorbed
9-20199	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R rabbit anti-mouseIgG(H+L), highly cross-adsorbed
9-20200	Biotium	EUR 283.20 EUR 290.40	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R rabbit anti-rat IgG(H+L), highly cross-adsorbed
9-20201	Biotium	EUR 283.20 EUR 290.40	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R goat anti-rabbit IgG(H+L)
9-20202	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A chicken anti-mouse IgG(H+L)
9-20208	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A chicken anti-rabbit IgG(H+L)
9-20209	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 chicken anti-mouse IgG(H+L)
9-20221	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 chicken anti-mouse IgG(H+L)
9-20222	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 chicken anti-rabbit IgG(H+L)
9-20223	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 chicken anti-rabbit IgG(H+L)
9-20224	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A chicken anti-goat IgG(H+L)
9-20225	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 chicken anti-goat IgG(H+L)
9-20226	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 chicken anti-goat IgG(H+L)
9-20227	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 goat anti-human IgG(H+L), highly cross-adsorbed
9-20230	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20231	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20232	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20233	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20234	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20235	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 goat anti-swine IgG(H+L)
9-20236	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF680 rabbit anti-guinea pig IgG(H+L)
9-20243	Biotium	EUR 306.00 EUR 312.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF770 rabbit anti-guinea pig IgG(H+L)
9-20244	Biotium	EUR 306.00 EUR 312.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20275	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF555 donkey anti-guinea pig IgG (H+L), highly cross-adsorbed
9-20276	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 goat anti-human IgG(H+L), highly cross-adsorbed
9-20280	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 goat anti-mouse IgG(H+L), highly cross-adsorbed
9-20281	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 goat anti-rabbit IgG(H+L), highly cross-adsorbed
9-20282	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20283	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20284	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20285	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF647 goat anti-swine IgG(H+L)
9-20286	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20293	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF568 Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20294	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20295	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20296	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20297	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Goat anti-mouse IgG (H+L), highly cross-adsorbed
9-20299	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Goat anti-rabbit IgG (H+L), highly cross-adsorbed
9-20300	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)
9-20301	Biotium	EUR 388.80 EUR 396.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)
9-20302	Biotium	EUR 388.80 EUR 396.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)
9-20303	Biotium	EUR 388.80 EUR 396.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)
9-20304	Biotium	EUR 388.80 EUR 396.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Donkey anti-mouse IgG(H+L), highly cross-adsorbed
9-20305	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-mouse IgG(H+L)
9-20306	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Rabbit anti-mouse IgG(H+L), highly cross-adsorbed
9-20307	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Donkey anti-rabbit IgG(H+L), highly cross-adsorbed
9-20308	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-rabbit IgG(H+L)
9-20309	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 donkey anti-chicken IgY (IgG) (H+L), highly cross-adsorbed
9-20310	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-chicken IgY(H+L), highly cross-adsorbed
9-20311	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 rabbit anti-chicken IgY (IgG) (H+L)
9-20312	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Bovine anti-goat IgG(H+L), highly cross-adsorbed
9-20313	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Donkey anti-goat IgG(H+L), highly cross-adsorbed
9-20314	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 rabbit anti-goat IgG(H+L)
9-20315	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 donkey anti-guinea pig IgG (H+L), highly cross-adsorbed
9-20316	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-guinea pig IgG(H+L)
9-20317	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 donkey anti-human IgG(H+L), highly cross-adsorbed
9-20318	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-human IgG(H+L), highly cross-adsorbed
9-20319	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 donkey anti-rat IgG(H+L), highly cross-adsorbed
9-20320	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-rat IgG(H+L), highly cross-adsorbed
9-20321	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 donkey anti-sheep IgG(H+L), highly cross-adsorbed
9-20322	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 rabbit anti-sheep IgG(H+L), highly cross-adsorbed
9-20323	Biotium	EUR 312.00 EUR 319.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-swine IgG(H+L)
9-20324	Biotium	EUR 271.20 EUR 277.20	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat)
9-20328	Biotium	EUR 388.80 EUR 396.00	0.5mL 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 Chicken Anti-Mouse IgG(H+L), 2 mg/mL
9-20331	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF350 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL
9-20332	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF543 Chicken Anti-Goat IgG(H+L), 2 mg/mL
9-20333	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF543 Chicken Anti-Mouse IgG(H+L), 2 mg/mL
9-20334	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF543 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL
9-20335	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF543 Rabbit Anit-Guinea Pig IgG(H+L), 2 mg/mL
9-20336	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF568 Chicken Anti-Goat IgG(H+L), 2 mg/mL
9-20337	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF568 Chicken Anti-Mouse IgG(H+L), 2 mg/mL
9-20338	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

CF568 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL
9-20339	Biotium	EUR 277.20 EUR 271.20 EUR 144.00	1mg 0.5mL 50uL
Description: Minimum order quantity: 1 unit of 50uL

Keywords: Birds; poultry; reproductive technologies; Semen analysis; Frozen sperm.