Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells
As a supplement widely used cell culture, fetal bovine serum (FBS) port of the high content of growth factors, proliferation, and adhesion. However, high cost, bio-safety, possible transmission of xenogeneic agents, restricted access, and ethical controversies major obstacle that prevents the use of these additives. Thus, new alternatives have been proposed by various pros and cons. However, caution must be taken in selecting a suitable replacement given that changes in the main aspects of cultured cells can be biased consequences of clinical applications.
Here, the authors ( Corneel Verburg) evaluated the impact of harvesting cord blood serum HES (CBS-HES), as an enriched source of growth factors, the mesenchymal stem cells basic (MSC) characteristics. In this experiment, umbilical MSCs cord-derived isolated and maintained with Dulbecco’s Modified Eagle containing either 10, 15, and 20% CBS-HES or FBSS Medium to compare their morphology, immunophenotype, growth and proliferation rate, the rate of death, cell cycle, and gene expression profiles. Despite all the media that support the expansion of MSC enriched with comparable morphology, cell surface markers, the mortality rate, c-MYC and p16 expression, and the growth rate, CBS-HES cells significantly (P <0.05) expressed more hTERT gene in concentration – a dependent manner.
However, no significant shift was observed in the cell cycle of cultured cells using the same concentration of additive, a finding that was subsequently confirmed by Ki-67 immunostaining.
CBS-HES as additives available and affordable, it seems to be optimal, it is relatively safe and promising alternative FBS for cultivation, propagation, and subsequent clinical application of MSC.
Dr. Djoumana Ounas
Keywords: alternative FBS; Fetal bovine serum; Hydroxyethyl starch; Mesenchymal stem cells; Umbilical cord.
Favorable Effects of Fetal Bovine Serum in Vitro Cryosurvival of Chicken spermatozoa
chicken spermatozoa are highly susceptible to cryopreservation often requires extender containing additives to improve the quality of post-thaw them. Although the protective properties of fetal bovine serum (FBS) for freezing cells cultured tissue is widely known, its potential as a cryoprotectant for most of the sperm cells have not been explored. Thus, the goal of our research is to (i) investigate the protective effect of FBS in different concentrations (0, 5, 10, 15 and 20%) to cryodamages in spermatozoa chicken, and (ii) to test the concentration of FBS that produces the best preservation compared 1 mg / mL of cholesterol-loaded cyclodextrins (CLC).
Samples were assessed before and after freezing on sperm motility parameters, plasma and integrities acrosome membrane, mitochondrial membrane potential, oxidative stress and peroxidation plasma membrane.
Our findings show that, despite their beneficial effects on fresh spermatozoa, the higher the concentration of FBS (15 and 20%) obtained the worst results for most motility and functional parameters after thawing. Conversely, lowering the concentration of FBS (5 and 10%) improved post-thaw all variables when compared with controls. After that, based on regression analysis, the concentration of 7% FBS have to be assessed against the CLC in the experiment was prepared by four groups: control, FBS, CLC, and FBS + CLC.
FBS and FBS + CLC group exhibited higher progressive motility in fresh samples, whereas only FBS higher retained post-thaw progressive motility. In addition, the incorporation FBS extender to increase the percentage of cells that quickly and reducing the production of free radicals and peroxidation plasma membrane. Together, these results indicate that FBS minimize some of the harmful effects of cryopreservation, providing an alternative to chicken semen extenders which seems superior in many aspects CLC at 1 mg / mL.
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Eotaxin-3/CCL26, human recombinant |
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Histone H4 Peptide, tetra-acetylated, biotin conjugate |
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FGF-5, human recombinant |
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IFN-?1, human recombinant |
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IFN-?2, human recombinant |
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 IGF-BP2, human recombinant |
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IGF-BP2, human recombinant |
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 IGF-BP4, human recombinant |
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IGF-BP4, human recombinant |
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IGF-BP7, human recombinant |
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 MCP-5/CCL12, murine recombinant |
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MCP-5/CCL12, murine recombinant |
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 MIP-1?/CCL3, human recombinant |
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MIP-1?/CCL3, human recombinant |
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MIP-1?/CCL9/10, murine recombinant |
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MIP-3/CCL23, human recombinant |
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Keywords: Birds; poultry; reproductive technologies; Semen analysis; Frozen sperm.
