chondroprogenitors articular, competitor suitable for cell-based therapies in cartilage repair, employing routine fetal bovine serum (FBS) for expansion and differentiation.
The possibility of transplant rejection or zoonotic transmission increases the need for xeno-free alternatives. The use of human platelet lysates (hPL), a nutritional supplement that is abundant in growth factors, have not been reported for human chondroprogenitor expansion so far.
Our aim was to compare the biological profile chondroprogenitors grow in hPL compared FBS.Chondroprogenitors 3 isolated from osteoarthritic knee joints. Here differential fibronectin adhesion assay, section 0 cells grow at (a) 10% FBS and (b) 10% hPL considered for the assessment of the growth kinetics, surface marker expression, gene expression and differentiation trilineage.
Latent transforming growth factor-β1 (TGFβ1) levels were also measured for each of the culture media used. RESULTS cell proliferation was significantly higher in cells grown with hPL (P <0.01). surface marker expression is comparable except in the CD-146 in which the group hPL has a value significantly higher (P = 0.03). Comparison of mRNA expression revealed lower values especially collagen I, collagen X, aggrecan and collagen II (P <0.05). trilineage differentiation seen in both groups with a high alizarin red absorption recorded in hPL. There is also a significantly higher level of latent TGFβ1 in medium containing hPL compared with FBS.
CONCLUSION This is the first in vitro study of xeno-free to assert that the hPL can serve as a supplement for optimal growth chondroprogenitors articular extension, although depth assessment and evaluation of population growth factor different dilutions of hPL is required to assess the suitability for use in translational research.
Comparison of Human Platelet Lysate versus Fetal Bovine Serum for Expansion of Human Articular Cartilage-Derived Chondroprogenitors.
Identification of novel copiparvovirus collected cow fetal bovine serum.
A novel parvovirus is identified as a contaminant of cell culture with metagenomic analysis. Digital droplet PCR (ddPCR) is used to determine viral load in the cell culture supernatant and further analysis, by ddPCR and DNA sequencing showed that fetal bovine serum (FBS) was used for cell culture is a source of contamination parvovirus.
The FBS novel contained ~ 50,000 copies per ml serum parvovirus DNA. DNA viruses that are resistant to DNAse digestion. near-full length novel parvovirus sequence determined. Phylogenetic analysis showed that the virus belongs to the genus Copiparvovirus, be closely linked to the bovine parvovirus 2 (BPV2) with 41% identity with the non-structural protein NS1 and 47% identity with the virus capsid protein of BPV2.
An individual screens and collected sera cow identify novel variants closely related virus in both serum pools. For classification purposes, the new virus has been designated species of cattle copiparvovirus 3 isolates JB9 (bocopivirus 3-JB9).
the different impact of charcoal-stripped fetal bovine serum in c-Myc between different subtypes of breast cancer cell lines.
Charcoal-stripped fetal bovine serum (CS-FBS) is often used in studies of hormone-responsive cancers to provide hormone-free cell culture conditions. CS-FBS can affect the growth of cancer cells; However, the underlying mechanisms remain unclear.
In this study, we aimed to clarify the effects of CS-FBS in different subtypes of breast cancer cells. We found that it is important c-Myc oncoprotein was significantly inhibited in estrogen receptor alpha (ER-α) positive breast cancer cells when cultured in medium CS-FBS-equipped, but it was not suppressed in the cells of ER-α-negative.
Description: Stromal cells are derived from fetal liver Mononuclear Cells. The fetal liver is homogenized, and the Mononuclear Cells are isolated using lymphocyte separation medium (1.077 g/ml density) and cultured. These cells have fibroblast and epithelial-like morphology
Description: Mouse Fetal Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Rat Mesenchymal (Fetal) Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Rat Lung Primary Mesenchymal Stem Cells are derived from the lung tissues of day-1 Sprague-Dawley Rats. Rat Lung Primary Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rat Lung Primary Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-6 passages by the ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD29 and CD44 positive by flow cytometry.
Description: Bone Marrow Stromal Cells (Mesenchymal Stem Cells) are derived from Bone Marrow-Mononuclear Cells from the first culture passage. Cells are derived from single donor
Description: Mouse Adult Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Feline Mesenchymal Stem Cells (FMSC) have the potential to maintain multipotency and proliferate extensively in vitro. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, mesenchymal steml cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSCs can differentiate into chondrocytes or osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-ad from Gentaur Research Laboratories are isolated from human adipose tissue. HMSC-ad are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-ad are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-ad are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-ad are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. The fetal liver is the source of early hematopoietic cells and considered as a source of pluripotent stem cells. MSCs can differentiate into chondrocytes or osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence, MSC can be cultured to expand their numbers and transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-he from Gentaur Research Laboratories are isolated from human liver. HMSC-he are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-he are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-he are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-he are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.
Description: Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. This property, in combination with MSC's developmental plasticity, has generated tremendous interest in the potential use of MSC to replace damaged tissues. Fetal lung has been identified as a rich source of MSCs and has been reported that MSCs can differentiate into neural cells in addition to their mesenchymal differentiation potential; enhance the engraftment of human umbilical cord blood-derived CD34 hematopoietic cells in nonobese diabetic-severe combined immunodeficiency mice. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC.HPMSC from Gentaur Research Laboratories are isolated from lung tissue. HPMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HPMSC are characterized by immunofluorescent method with antibodies to CD73, CD90 and CD105. HPMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMSC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult CD1 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult B129 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.
Description: Rat Adult Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Mouse Bone Marrow Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Cynomolgus Monkey Bone Marrow Mesenchymal Stem Cells are derived from the tibias of Cynomolgus Monkey. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44 and CD29 by flow cytometry.
Description: Canine Bone Marrow Mesenchymal Stem Cells are derived from the tibias of beagle dog. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.
Description: Rabbit Bone Marrow Mesenchymal Stem Cells are derived from the tibias of New Zealand Whit Rabbit. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 2-3 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44, CD90 or CD29 by flow cytometry.
Description: Human Mesenchymal (Liver) Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Bone Marrow Stromal Cells (Mesenchymal Stem Cells) are derived from Bone Marrow-Mononuclear Cells from the first culture passage. Cells are derived from multiple donors and mixed
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSCs can differentiate into chondrocytes or osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-bm from Gentaur Research Laboratories are isolated from human bone marrow. HMSC-bm are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-bm are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-bm are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-bm are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.
Description: Porcine Bone Marrow Mesenchymal Stem Cells are derived from porcine tibias. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44 and CD29 by flow cytometry.
Description: Hamster Bone Marrow Mesenchymal Stem Cells are derived from the tibias of Syrian hamster. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 2-3 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44, CD90 or CD29 by flow cytometry.
Description: Human Whartons Jelly Mesenchymal Stem Cells provide an ideal culture model for the study of multipotent stem cell biology and differentiation processes. HMSC-WJ are isolated from Wharton's Jelly of human umbilical cord and cryopreserved to ensure optimal phenotype and the highest viability and plating efficiency. HMSC-WJ can be expanded in an undifferentiated state for future differentiation to multiple lineages. Human Mesenchymal Stem Cells may be differentiated down the typical mesenchymal lineages, such as adipogenic, chondrogenic, and osteogenic. Human Whartons Jelly Mesenchymal Stem Cells are characterized by flow cytometry to ensure the proper expression of multiple markers of mesenchymal stem cells. They are uniformly positive for CD29, CD44, CD73, CD90, CD105, and CD166. They are uniformly negative for CD14, CD31, CD34, and CD45. Gentaur has developed optimized media to expand the Human Mesenchymal Stem Cell products in the undifferentiated state, as well as optimized media kits for inducing Adipogenesis, Chondrogenesis, and Osteogenesis. Additionally, provides convenient staining kits for staining the fully differentiated cells.
Description: Derived from inguinal fat tissue of beagle dogs (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium. Cells are cryopreserved at the second passage.
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult C57BL/6 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur.
Description: Human Pre-Adipocyte MSCs from a single adult donor. Gentaur human mesenchymal stem cells have been functionally tested and are compatible with our MSC Expansion Medium. Our mesenchymal stem cells are andgt;95% positive for the presence of CD29, CD44, CD73, CD90, CD105, CD166 markers by flow cytometry analysis. Our MSCs are andlt;2% positive for the presence of CD14, CD31, CD34, CD45 markers by flow cytometry analysis. All of the cells provided are tested and are negative for HIV-1, HIV-2, Hepatitis B and Hepatitis C as detected by PCR. The cells are confirmed to be negative for mycoplasma and other detectable microbial contamination.
HighQC™ Human Placental Derived Mesenchymal Stem Cell
Description: As interest grows in the expanding field of stem cell research, areas such as regenerative medicine, tissue engineering and basic stem cell biology necessitate a reliable cost-effective cell-based tool for use in discovery and design projects. Adipose-derived adult stem cells and their unique differentiation properties can be utilized in any number of research applications and offer decided benefits over similar multipotent cell types.Adipose tissue is typically in greater supply enabling ample sourcing for isolation and production of adult stem cells – at a fraction of the cost for bone marrow-derived counterparts while maintaining similar markers and characteristics. Adult stem cells offer another more subtle benefit allowing scientists to circumvent the ethical issues normally associated with employing embryonic stem cells in biomedical research.Gentaur adult stem cells are available with optimized media formulations to maximize growth, survival and differentiation in culture. All cells have been screened negative for HIV-1, HIV-2, HTLC-1, HTLV-2, Hep-B, Hep-C, and mycoplama.
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Description: Derived from the inguinal fat tissue of New Zealand white rabbits (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium. Cells are cryopreserved at the second passage.
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Description: Rat Bone Marrow Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Human Bone Marrow Derived Mesenchymal Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent.Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms.Optimizing your hMSC Cultures: 1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..2) Use of our MSCGroTM media is highly recommended.3) Subtrate-We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.
Exosome derived from human preadipocyte (Mesenchymal Stem Cell)
Description: HAdMSCs are isolated from lipoaspirate derived from white adipose tissue and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HAdMSCs are puromycin resistant HAdMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HAdMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.
Description: Derived from fat tissue of strain Wistar rats (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium.Cells are cryopreserved at the second passage.
Exosome derived from hTERT-immortalized Mesenchymal Stem Cell
Description: Human Mesenchymal (Adipose Derived) Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
Description: Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Description: Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
C57BL - 6 Mouse Bone Marrow Mesenchymal Stem Cells
Description: These cells are native human MSCs labeled with rhodamine (Excitation = 550 nm; Emission = 570 nm) through a cell membrane integrated probe. This product is useful in tracking studies of MSCs such as homing to specific targets and the mechanisms of this homing. They are provided as 500,000 cryopreserved cells.This product requires shipment on dry ice and immediate transfer to liquid nitrogen storage or culture. Please note there is a 33 USD dry ice charge in addition to Fedex next day shipping.Optimizing your hMSC Cultures: 1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..2) Use of our MSCGroTM media is highly recommended.3) Subtrate-We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.
MSCGro-Humanized Mesenchymal Stem Cell Clinical Grade Medium, SF
The addition of 17β-estradiol (E2) to the CS-FBS media-equipped saved CS-FBS-inhibition of c-Myc-induced, whereas treatment with 5α-dihydrotestosterone (DHT) suppressed the expression of c-Myc. Our data show that CS-FBS to inhibit the growth of breast cancer cells ER-α-positive through inhibition of c-Myc, and this is probably due to the elimination estro
