chondroprogenitors articular, competitor suitable for cell-based therapies in cartilage repair, employing routine fetal bovine serum (FBS) for expansion and differentiation.
The possibility of transplant rejection or zoonotic transmission increases the need for xeno-free alternatives. The use of human platelet lysates (hPL), a nutritional supplement that is abundant in growth factors, have not been reported for human chondroprogenitor expansion so far.
Our aim was to compare the biological profile chondroprogenitors grow in hPL compared FBS.Chondroprogenitors 3 isolated from osteoarthritic knee joints. Here differential fibronectin adhesion assay, section 0 cells grow at (a) 10% FBS and (b) 10% hPL considered for the assessment of the growth kinetics, surface marker expression, gene expression and differentiation trilineage.
Latent transforming growth factor-β1 (TGFβ1) levels were also measured for each of the culture media used. RESULTS cell proliferation was significantly higher in cells grown with hPL (P <0.01). surface marker expression is comparable except in the CD-146 in which the group hPL has a value significantly higher (P = 0.03). Comparison of mRNA expression revealed lower values especially collagen I, collagen X, aggrecan and collagen II (P <0.05). trilineage differentiation seen in both groups with a high alizarin red absorption recorded in hPL. There is also a significantly higher level of latent TGFβ1 in medium containing hPL compared with FBS.
CONCLUSION This is the first in vitro study of xeno-free to assert that the hPL can serve as a supplement for optimal growth chondroprogenitors articular extension, although depth assessment and evaluation of population growth factor different dilutions of hPL is required to assess the suitability for use in translational research.
Comparison of Human Platelet Lysate versus Fetal Bovine Serum for Expansion of Human Articular Cartilage-Derived Chondroprogenitors.
Identification of novel copiparvovirus collected cow fetal bovine serum.
A novel parvovirus is identified as a contaminant of cell culture with metagenomic analysis. Digital droplet PCR (ddPCR) is used to determine viral load in the cell culture supernatant and further analysis, by ddPCR and DNA sequencing showed that fetal bovine serum (FBS) was used for cell culture is a source of contamination parvovirus.
The FBS novel contained ~ 50,000 copies per ml serum parvovirus DNA. DNA viruses that are resistant to DNAse digestion. near-full length novel parvovirus sequence determined. Phylogenetic analysis showed that the virus belongs to the genus Copiparvovirus, be closely linked to the bovine parvovirus 2 (BPV2) with 41% identity with the non-structural protein NS1 and 47% identity with the virus capsid protein of BPV2.
An individual screens and collected sera cow identify novel variants closely related virus in both serum pools. For classification purposes, the new virus has been designated species of cattle copiparvovirus 3 isolates JB9 (bocopivirus 3-JB9).
the different impact of charcoal-stripped fetal bovine serum in c-Myc between different subtypes of breast cancer cell lines.
Charcoal-stripped fetal bovine serum (CS-FBS) is often used in studies of hormone-responsive cancers to provide hormone-free cell culture conditions. CS-FBS can affect the growth of cancer cells; However, the underlying mechanisms remain unclear.
In this study, we aimed to clarify the effects of CS-FBS in different subtypes of breast cancer cells. We found that it is important c-Myc oncoprotein was significantly inhibited in estrogen receptor alpha (ER-α) positive breast cancer cells when cultured in medium CS-FBS-equipped, but it was not suppressed in the cells of ER-α-negative.
Description: Fetal human brain stem tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human brain stem tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain stem tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain stem tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
Human Serum Albumin (HSA), Cell Culture Tested, Recombinant from plant
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls.
Total Protein - Murine Embryonic Stem Cell Line D3
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Stem Cell Factor (SCF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Stem Cell Factor (SCF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Description: DiscoveryProbe? FDA-approved drug library includes 1496 FDA approved drugs for high throughput screening (HTS) and high content screening (HCS). It can be used to find new targets for old drugs. The bioactivity and safety of these drugs were confirmed by clinical trials.
Description: DiscoveryProbe? FDA-approved drug library includes 1496 FDA approved drugs for high throughput screening (HTS) and high content screening (HCS). It can be used to find new targets for old drugs. The bioactivity and safety of these drugs were confirmed by clinical trials.
Description: DiscoveryProbe? FDA-approved drug library includes 1496 FDA approved drugs for high throughput screening (HTS) and high content screening (HCS). It can be used to find new targets for old drugs. The bioactivity and safety of these drugs were confirmed by clinical trials.
Description: DiscoveryProbe? FDA-approved drug library includes 1496 FDA approved drugs for high throughput screening (HTS) and high content screening (HCS). It can be used to find new targets for old drugs. The bioactivity and safety of these drugs were confirmed by clinical trials.
The addition of 17β-estradiol (E2) to the CS-FBS media-equipped saved CS-FBS-inhibition of c-Myc-induced, whereas treatment with 5α-dihydrotestosterone (DHT) suppressed the expression of c-Myc. Our data show that CS-FBS to inhibit the growth of breast cancer cells ER-α-positive through inhibition of c-Myc, and this is probably due to the elimination estro
