Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

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Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.

Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.

Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.

In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.

Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.

In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).

Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.

FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).

Fetal Bovine Serum, Premium, US Origin
F0600-050 500ml
EUR 789.6

Chondrogenic Differentiation Medium For Human Fetal Bone Marrow Mesenchymal Stem Cells
HUXMX-90041 100mL
EUR 409

Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90061 500mL
EUR 399

Fetal Bovine Serum, Premium, US Origin, Dialyzed
F0602-050 500ml
EUR 1275.6

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760-1 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760-10 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760S - Ask for price

Serum Free Medium For Human Adipose-derived Mesenchymal Stem Cells （Type I）
HUXMD-90061 500mL
EUR 579

Human Mesenchymal Stem Cells
SC00A1 500000
EUR 1086

Fetal Bovine Serum, Premium, US Origin , Heat Inactivated
F0601-050 500ml
EUR 837.6

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered US Origin
SLS1154 500ML
EUR 1120.2

Hyclone Insect Cell Screened Fetal Bovine Serum US Origin 500 mL (SH30070.03I)
HYC28 EACH
EUR 1156.76

Bovine Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS), BSE-TSE free (Australian Origin), Low endotoxin
FETB16-N-1 1 g
EUR 270

Hyclone Defined Fetal Bovine Serum US Origin 100 mL (SH30070.02)
HYC17 EACH
EUR 228

Hyclone Defined Fetal Bovine Serum US Origin 500 mL (SH30070.03)
HYC23 EACH
EUR 946.8

Hyclone Dialyzed Fetal Bovine Serum US Origin 100 mL (SH30079.02)
HYC45 EACH
EUR 174

Hyclone Dialyzed Fetal Bovine Serum US Origin 500 mL (SH30079.03)
HYC47 EACH
EUR 726

Hyclone Standard Fetal Bovine Serum US Origin 100 mL (SH30088.02)
HYC56 EACH
EUR 124.8

Hyclone Standard Fetal Bovine Serum US Origin 500 mL (SH30088.03)
HYC58 EACH
EUR 522

Rat mesenchymal Stem cell Flow Cytometry Kit
RAXMX-09011 10 tests
EUR 1079

Hyclone Characterized Fetal Bovine Serum US Origin 100 mL (SH30071.02)
HYC37 EACH
EUR 154.8

Hyclone Characterized Fetal Bovine Serum US Origin 500 mL (SH30071.03)
HYC39 EACH
EUR 637.2

Hyclone Defined Fetal Bovine Serum US Origin 50 mL (SH30070.01)
HYC15 EACH
EUR 134.4

Human mesenchymal Stem cell Flow Cytometry Kit
HUXMX-09011 10 tests
EUR 1359

Mouse mesenchymal Stem cell Flow Cytometry Kit
MUXMX-09011 10 tests
EUR 799

Mesenchymal Stem Cells - Adipose Derived
SC00AD1 500,000 Cells
EUR 1086

Hyclone Dialyzed Fetal Bovine Serum US Origin 50 mL (SH30079.01)
HYC43 EACH
EUR 103.2

MSCGro-Humanized Mesenchymal Stem Cell, complete
SC00B2-500 500 ml
EUR 450

Mesenchymal Stem Cells - Placental Derived
SC00P1 500,000 cells
EUR 1086

Hyclone Super Low IgG Fetal Bovine Serum US Origin 100 mL (SH30898.02)
HYC72 EACH
EUR 192

Hyclone Super Low IgG Fetal Bovine Serum US Origin 500 mL (SH30898.03)
HYC75 EACH
EUR 794.4

Human Bone Marrow Mesenchymal Stem Cells
HUXMA-01001 1*10^6/vial
EUR 1030

Hyclone Characterized Fetal Bovine Serum US Origin 50 mL (SH30071.01)
HYC35 EACH
EUR 90

Canine Bone Marrow Mesenchymal Stem Cells
CAXMX-01001 1*10^6/vial
EUR 999

Rabbit Bone Marrow Mesenchymal Stem Cells
RBXMX-01001 1*10^6/vial
EUR 649

Mesenchymal Stem Cells - Bone Marrow Derived
SC00BM1 500,000 cells
EUR 1086

SD Rat Bone Marrow Mesenchymal Stem Cells
RASMX-01001 1*10^6/vial
EUR 479

Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-01001 1*10^6/vial
EUR 1159

Hyclone Tetracycline Screened Fetal Bovine Serum US Origin 500 mL (SH30070.03T)
HYC32 EACH
EUR 1156.76

F344 Rat Bone Marrow Mesenchymal Stem Cells
RAFMX-01001 1*10^6/vial
EUR 479

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Heat Inactivated US Origin
SLS1156 500ML
EUR 1096.7

Human Adipose-derived Mesenchymal Stem Cells
HUXMD-01001 1*10^6/vial
EUR 1079

Wistar Rat Bone Marrow Mesenchymal Stem Cells
RAWMX-01001 1*10^6/vial
EUR 479

Canine Adipose-derived Mesenchymal Stem Cells
CAXMD-01001 1*10^6/vial
EUR 1200

Rabbit Adipose-derived Mesenchymal Stem Cells
RBXMD-01001 1*10^6/vial
EUR 799

FETAL BOVINE SERUM, REGULAR, NEW ZEALAND ORIGIN, GAMMA IRRADIATED, 500 ML
35-082-CV 500 mL/pk
EUR 1296
Description: Serum; Serum

Hyclone Research Grade Fetal Bovine Serum SouthAmerican Origin 100 mL (SV30160.02)
HYC83 EACH
EUR 73.2

Hyclone Research Grade Fetal Bovine Serum SouthAmerican Origin 500 mL (SV30160.03)
HYC85 EACH
EUR 308.4

Human Mesenchymal Stem Cells Expressing Oct3/4
SC00A6 500000
EUR 1420.8

SD Rat Adipose-derived Mesenchymal Stem Cells
RASMD-01001 1*10^6/vial
EUR 639

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 500 mL (SH30068.03)
HYC11 EACH
EUR 733.2

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 100 mL (SH30068.02)
HYC9 EACH
EUR 176.4

Human Mesenchymal Stem Cells - Bone Marrow Derived
MSC001 500,000+ Cells
EUR 1545.6

Rat Bone Marrow Mesenchymal Stem Cells With GFP
RBXMX-01101 1*10^6/vial
EUR 1089

Rat Bone Marrow Mesenchymal Stem Cells With RFP
RBXMX-01201 1*10^6/vial
EUR 1089

Human Umbilical Cord Blood Mesenchymal Stem Cells
HUXUB-01001 1*10^6/vial
EUR 1159

Balb/c Mouse Bone Marrow Mesenchymal Stem Cells
MUCMX-01001 1*10^6/vial
EUR 639

Hyclone Characterized Fetal Bovine Serum US Origin Irrad 500 mL (SH30071.03IR)
HYC42 EACH
EUR 666

Hyclone Dialyzed Fetal Bovine Serum US Origin Irradiated 500 mL (SH30079.03IR)
HYC50 EACH
EUR 793.2

Human Bone Marrow Mesenchymal Stem Cells With GFP
HUXMA-01101 1*10^6/vial
EUR 1479

Human Bone Marrow Mesenchymal Stem Cells With RFP
HUXMA-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells
MUBMX-01001 1*10^6/vial
EUR 639

Hyclone Defined Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30070.02HI)
HYC20 EACH
EUR 255.6

Hyclone Defined Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30070.03HI)
HYC27 EACH
EUR 966

Wistar Rat Adipose-derived Mesenchymal Stem Cells
RAWMD-01001 1*10^6/vial
EUR 639

Canine Bone Marrow Mesenchymal Stem Cells With GFP
CAXMX-01101 1*10^6/vial
EUR 1229

Canine Bone Marrow Mesenchymal Stem Cells With RFP
CAXMX-01201 1*10^6/vial
EUR 1229

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 50 mL (SH30079.01HI)
HYC44 EACH
EUR 122.4

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30079.02HI)
HYC46 EACH
EUR 194.4

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30079.03HI)
HYC48 EACH
EUR 771.6

Hyclone Standard Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30088.02HI)
HYC57 EACH
EUR 144

Hyclone Standard Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30088.03HI)
HYC59 EACH
EUR 541.2

SD Rat Bone Marrow Mesenchymal Stem Cells With GFP
RASMX-01101 1*10^6/vial
EUR 929

SD Rat Bone Marrow Mesenchymal Stem Cells With RFP
RASMX-01201 1*10^6/vial
EUR 929

Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7
T0352 1x106 cells / 1.0 ml Ask for price

Hyclone Super Low IgG Fetal Bovine Serum US Origin Irrad 500 mL (SH30898.03IR)
HYC78 EACH
EUR 813.6

Hyclone Characterized Fetal Bovine Serum US Origin HeatInact 100 mL (SH30071.02HI)
HYC38 EACH
EUR 174

Hyclone Characterized Fetal Bovine Serum US Origin HeatInact 500 mL (SH30071.03HI)
HYC40 EACH
EUR 658.8

Human Umbilical Cord Mesenchymal Stem Cells With GFP
HUXUC-01101 1*10^6/vial
EUR 1599

F344 Rat Bone Marrow Mesenchymal Stem Cells With GFP
RAFMX-01101 1*10^6/vial
EUR 929

F344 Rat Bone Marrow Mesenchymal Stem Cells With RFP
RAFMX-01201 1*10^6/vial
EUR 929

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 50 mL (SH30068.01)
HYC7 EACH
EUR 103.2

Balb/c Mouse Adipose-derived Mesenchymal Stem Cells
MUCMD-01001 1*10^6/vial
EUR 649

MSCGro-Humanized Mesenchymal Stem Cell Clinical Grade Medium, SF
SC00B4-500 500 ml
EUR 434.4

Hyclone ES Screened Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30070.02EH)
HYC19 EACH
EUR 257.64

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled
SC00A3 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc
T0169 1x106 cells / 1.0 ml Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras
T0170 1x106 cells / 1.0 ml Ask for price

Wistar Rat Bone Marrow Mesenchymal Stem Cells With GFP
RAWMX-01101 1*10^6/vial
EUR 929

Wistar Rat Bone Marrow Mesenchymal Stem Cells With RFP
RAWMX-01201 1*10^6/vial
EUR 929

Hyclone Defined Fetal Bovine Serum US Origin Heat Inactivated 50 mL (SH30070.01HI)
HYC16 EACH
EUR 153.6

Hyclone Characterized Fetal Bovine Serum US Origin Heat Inact 50 mL (SH30071.01HI)
HYC36 EACH
EUR 109.2

Human Adipose-derived Mesenchymal Stem Cells With GFP
HUXMD-01101 1*10^6/vial
EUR 1479

Human Adipose-derived Mesenchymal Stem Cells With RFP
HUXMD-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Adipose-Derived Mesenchymal Stem Cells
MUBMD-01001 1*10^6/vial
EUR 649

Hyclone Super Low IgG Fetal Bovine Serum US Origin HeatInact 100 mL (SH30898.02HI)
HYC73 EACH
EUR 212.4

Hyclone Super Low IgG Fetal Bovine Serum US Origin HeatInact 500 mL (SH30898.03HI)
HYC76 EACH
EUR 813.6

Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40
T0167 1x106 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells-Green Fluorescent-Labeled
SC00A2 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT
T0168 1x106 cells / 1.0 ml Ask for price

SD Rat Adipose-derived Mesenchymal Stem Cells With GFP
RASMD-01101 1*10^6/vial
EUR 1239

SD Rat Adipose-derived Mesenchymal Stem Cells With RFP
RASMD-01201 1*10^6/vial
EUR 1239

Immortalized Human Bone Marrow Mesenchymal Stem Cells SV40
T0520 1x106 cells / 1.0 ml Ask for price

Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP
MUCMX-01101 1*10^6/vial
EUR 1079

Human Umbilical Cord Blood Mesenchymal Stem Cells With GFP
HUXUB-01101 1*10^6/vial
EUR 1599

Complete Medium For Rat Bone Marrow Mesenchymal Stem Cells
RAXMX-90011 500mL
EUR 289

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP
MUBMX-01101 1*10^6/vial
EUR 1079

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With RFP
MUBMX-01201 1*10^6/vial
EUR 1079

Complete Medium For Human Bone Marrow Mesenchymal Stem Cells
HUXMA-90011 500mL
EUR 319

Complete Medium For Mouse Bone Marrow Mesenchymal Stem Cells
MUXMX-90011 500mL
EUR 289

Complete Medium For Canine Bone Marrow Mesenchymal Stem Cells
CAXMX-90011 500mL
EUR 319

Complete Medium For Monkey Bone Marrow Mesenchymal Stem Cells
MKXMA-90011 500mL
EUR 289

Complete Medium For Rabbit Bone Marrow Mesenchymal Stem Cells
RBXMX-90011 500mL
EUR 289

Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90011 500mL
EUR 319

Complete Medium For Rat Adipose-derived Mesenchymal Stem Cells
RAXMD-90011 500mL
EUR 289

C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP
MUBMD-01101 1*10^6/vial
EUR 1239

Complete Medium For Canine Adipose-derived Mesenchymal Stem Cells
CAXMD-90011 500mL
EUR 289

Complete Medium For Human Adipose-derived Mesenchymal Stem Cells
HUXMD-90011 500mL
EUR 319

Complete Medium For Monkey Adipose-derived Mesenchymal Stem Cells
MKXMD-90011 500mL
EUR 289

Complete Medium For Mouse Adipose-derived Mesenchymal Stem Cells
MUXMD-90011 500mL
EUR 289

Complete Medium For Rabbit Adipose-derived Mesenchymal Stem Cells
RBXMD-90011 500mL
EUR 289

Complete Medium For Human Umbilical Cord Blood Mesenchymal Stem Cells
HUXUB-90011 500mL
EUR 319

Osteogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells
RAXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells
RAXMX-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells
HUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells
HUXMX-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells
MUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells
MUXMX-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells
RAXMX-90041 100mL
EUR 409

Chondrogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells
HUXMD-90041 100mL
EUR 409

Hepatogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells
HUXMX-90101 50+100+200mL
EUR 759

Chondrogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells
MUXMX-90041 100mL
EUR 409

PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes
EXOP-140A-1 50 ug
EUR 525

Mesenchymal Cell Kit
abx290006-50test 50 test
EUR 2949.6

Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells
RAXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells
RAXMD-90031 200+100mL
EUR 359

Osteogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells
HUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells
HUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90041 100mL
EUR 409

Osteogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells
MUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells
MUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells
RAXMD-90041 100mL
EUR 409

PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes
EXOP-145A-1 50 ug
EUR 525

Osteogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)
GUXMX-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)
GUXMX-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose)
GUXMX-90041 100mL
EUR 409

Chondrogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells
MUXMD-90041 100mL
EUR 409

Osteogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)
GUXMD-90021 200mL
EUR 250

Adipogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)
GUXMD-90031 200+100mL
EUR 359

Chondrogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose)
GUXMD-90041 100mL
EUR 409

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested
CA068-010 100g
EUR 375.6

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested
CA068-050 500g
EUR 1170

HQ Fetal Bovine Serum
abx098128-500ml 500 ml
EUR 895.2

EQ Fetal Bovine Serum
abx298025-500ml 500 ml
EUR 1362

FBS - Fetal Bovine Serum
FBS001 500 ml
EUR 654

Vimentin (Mesenchymal Cell Marker) (VM452) Antibody
BNUB0452-50 50uL
EUR 486
Description: Primary antibody against Vimentin (Mesenchymal Cell Marker) (VM452), 1mg/mL

Fetal Bovine Serum, Triple Low
F0610-050 500ml Ask for price

GOOD fetal bovine serum - 500 mL
DDP40-37500 EACH
EUR 144.78

Fetal Bovine Serum（Best-Quality ）
FBSAD-01011 100/500mL Ask for price

Fetal Bovine Serum (FBS) [50 ml]
ABP-MED-FBS0050 50ml Ask for price

Fetal Bovine Serum (FBS) [500 ml]
ABP-MED-FBS0500 500ml Ask for price

Fetal Bovine Serum（Without Exosome ）
FBSNE-01061 20/50mL Ask for price

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)
RA0338-C.1 0.1 ml
EUR 150

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)
RA0338-C.5 0.5 ml
EUR 296.4

After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.

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