Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.
Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.
Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.
In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.
Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.
In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).
Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.
FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).
 iFluor™ 610 succinimidyl ester |
|
1038 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 iFluor™ 546 succinimidyl ester |
|
1048 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 iFluor™ 568 succinimidyl ester |
|
1049 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates* |
|
11001 |
AAT Bioquest |
1 L |
EUR 202 |
|
|
 ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates* |
|
11003 |
AAT Bioquest |
1 L |
EUR 480 |
|
|
 ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution* |
|
11004 |
AAT Bioquest |
5x10 mL |
EUR 132 |
|
|
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates* |
|
11012 |
AAT Bioquest |
100 ml |
EUR 115 |
|
|
 ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1105 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* |
|
11050 |
AAT Bioquest |
1 g |
EUR 115 |
|
|
 Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence* |
|
11100 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence* |
|
11105 |
AAT Bioquest |
500 tests |
EUR 219 |
|
|
 ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1114 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1120 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1123 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1126 |
AAT Bioquest |
2 Labelings |
EUR 50 |
|
|
 Amplite™ Colorimetric Glucose Oxidase Assay Kit |
|
11299 |
AAT Bioquest |
500 Tests |
EUR 306 |
|
|
 ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1130 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence* |
|
11300 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence* |
|
11301 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence* |
|
11302 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence* |
|
11303 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence* |
|
11304 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Assay Kit) Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit |
|
11305 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence* |
|
11306 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Colorimetric Xanthine Oxidase Assay Kit |
|
11307 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Colorimetric Acetylcholinesterase Assay Kit |
|
11400 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* |
|
11401 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence* |
|
11402 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence* |
|
11403 |
AAT Bioquest |
200 Tests |
EUR 219 |
|
|
 Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence* |
|
11503 |
AAT Bioquest |
200 Tests |
EUR 263 |
|
|
 Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence* |
|
11504 |
AAT Bioquest |
100 Tests |
EUR 263 |
|
|
 Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry* |
|
11505 |
AAT Bioquest |
100 Tests |
EUR 263 |
|
|
 Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry* |
|
11506 |
AAT Bioquest |
100 Tests |
EUR 263 |
|
|
 Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* |
|
11540 |
AAT Bioquest |
1000 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* |
|
11541 |
AAT Bioquest |
1000 Tests |
EUR 219 |
|
|
 Assay Kit *Blue Color*) Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color* |
|
11551 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Assay Kit *Red Fluorescence*) Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence* |
|
11552 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Assay Kit *Near Infrared Fluorescence*) Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence* |
|
11553 |
AAT Bioquest |
500 Tests |
EUR 306 |
|
|
 Assay Kit) Amplite™ Luminometric Peroxidase (HRP) Assay Kit |
|
11559 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence* |
|
11560 |
AAT Bioquest |
200 Tests |
EUR 306 |
|
|
 FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* |
|
11600 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* |
|
11610 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* |
|
11612 |
AAT Bioquest |
10 g |
EUR 898 |
|
|
 MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5* |
|
11614 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5* |
|
11617 |
AAT Bioquest |
5 g |
EUR 480 |
|
|
 pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9* |
|
11619 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
 CF-MUP, sodium salt *Superior alternative to MUP* |
|
11628 |
AAT Bioquest |
10 mg |
EUR 50 |
|
|
 Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color* |
|
11950 |
AAT Bioquest |
500 Tests |
EUR 176 |
|
|
 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* |
|
11952 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence* |
|
11953 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence* |
|
11954 |
AAT Bioquest |
500 Tests |
EUR 219 |
|
|
 Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence* |
|
11956 |
AAT Bioquest |
100 Tests |
EUR 219 |
|
|
 ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1227 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1235 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1240 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1245 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]) Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid] |
|
12481 |
AAT Bioquest |
25 mg |
EUR 219 |
|
|
Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid] |
|
12482 |
AAT Bioquest |
100 mg |
EUR 480 |
|
|
 ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1250 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 D-Luciferin, free acid *CAS#: 2591-17-5* |
|
12501 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
D-Luciferin, free acid *CAS#: 2591-17-5* |
|
12502 |
AAT Bioquest |
100 mg |
EUR 132 |
|
|
D-Luciferin, free acid *CAS#: 2591-17-5* |
|
12503 |
AAT Bioquest |
1 g |
EUR 528 |
|
|
 D-Luciferin, potassium salt *CAS#: 115144-35-9* |
|
12505 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
D-Luciferin, potassium salt *CAS#: 115144-35-9* |
|
12506 |
AAT Bioquest |
100 mg |
EUR 132 |
|
|
D-Luciferin, potassium salt *CAS#: 115144-35-9* |
|
12507 |
AAT Bioquest |
1 g |
EUR 263 |
|
|
 D-Luciferin, sodium salt *CAS#: 103404-75-7* |
|
12509 |
AAT Bioquest |
25 mg |
EUR 115 |
|
|
D-Luciferin, sodium salt *CAS#: 103404-75-7* |
|
12510 |
AAT Bioquest |
100 mg |
EUR 132 |
|
|
D-Luciferin, sodium salt *CAS#: 103404-75-7* |
|
12511 |
AAT Bioquest |
1 g |
EUR 528 |
|
|
 D-Luciferin phosphate *CAS 145613-12-3* |
|
12512 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 D-Luciferin galactoside *CAS 131474-38-9* |
|
12513 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 D-Luciferin methyl ester *CAS 73918-26-0* |
|
12514 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 D-Luciferin ethyl ester *CAS 135251-85-3* |
|
12515 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12518 |
AAT Bioquest |
1 plate |
EUR 176 |
|
|
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12519 |
AAT Bioquest |
10 plates |
EUR 393 |
|
|
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12520 |
AAT Bioquest |
100 plates |
EUR 2616 |
|
|
 Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12530 |
AAT Bioquest |
1 plate |
EUR 219 |
|
|
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12531 |
AAT Bioquest |
10 Plates |
EUR 702 |
|
|
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12532 |
AAT Bioquest |
100 plates |
EUR 3486 |
|
|
 Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12535 |
AAT Bioquest |
1 plate |
EUR 219 |
|
|
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12536 |
AAT Bioquest |
10 plates |
EUR 876 |
|
|
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
|
12537 |
AAT Bioquest |
100 plates |
EUR 3486 |
|
|
 ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1255 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit |
|
12551 |
AAT Bioquest |
200 Tests |
EUR 393 |
|
|
guanine]) BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine] |
|
12560 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine] |
|
12561 |
AAT Bioquest |
5 mg |
EUR 393 |
|
|
 ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1260 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* |
|
12601 |
AAT Bioquest |
500 Tests |
EUR 306 |
|
|
 Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence* |
|
12602 |
AAT Bioquest |
200 Tests |
EUR 306 |
|
|
 Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence* |
|
12603 |
AAT Bioquest |
200 Tests |
EUR 306 |
|
|
 Amplite™ Colorimetric Beta-Galactosidase Assay Kit |
|
12604 |
AAT Bioquest |
200 Tests |
EUR 306 |
|
|
 HIS Lite™ Cy3 Bis NTA-Ni Complex |
|
12610 |
AAT Bioquest |
1 mg |
EUR 393 |
|
|
 HIS Lite™ Cy5 Bis NTA-Ni Complex |
|
12613 |
AAT Bioquest |
1 mg |
EUR 393 |
|
|
 ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1265 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1290 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1292 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1294 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* |
|
1299 |
AAT Bioquest |
2 Labelings |
EUR 176 |
|
|
 ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction* |
|
1300 |
AAT Bioquest |
2 Labelings |
EUR 876 |
|
|
 Benzoyl-Leu-Gly-Arg-Aminoluciferin |
|
13000 |
AAT Bioquest |
1 mg |
EUR 480 |
|
|
 BOC-Leu-Gly-Arg-Aminoluciferin |
|
13001 |
AAT Bioquest |
1 mg |
EUR 480 |
|
|
 BOC-Val-Pro-Arg-Aminoluciferin |
|
13002 |
AAT Bioquest |
1 mg |
EUR 480 |
|
|
 D-VLK-AMC [D-Val-Leu-Lys-AMC] |
|
13201 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 Ac-DEVD-AFC *CAS 201608-14-2* |
|
13401 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 Ac-DEVD-AMC *CAS 169332-61-0* |
|
13402 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 Ac-DEVD-CHO *CAS 169332-60-9* |
|
13403 |
AAT Bioquest |
1 mg |
EUR 132 |
|
|
 Ac-DEVD-pNA *CAS 189950-66-1* |
|
13405 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 Ac-IETD-AFC *CAS 211990-57-7* |
|
13410 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 Ac-IETD-CHO *CAS 191338-86-0* |
|
13412 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 Z-IETD-pNA *CAS 219138-21-3* |
|
13413 |
AAT Bioquest |
5 mg |
EUR 219 |
|
|
 Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction* |
|
1342 |
AAT Bioquest |
2 Labelings |
EUR 306 |
|
|
 Z-IETD-AFC *CAS 219138-02-0* |
|
13425 |
AAT Bioquest |
5 mg |
EUR 158 |
|
|
 Ac-LEHD-AMC *CAS 292633-16-0* |
|
13426 |
AAT Bioquest |
5 mg |
EUR 176 |
|
|
 Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8* |
|
13445 |
AAT Bioquest |
10 mg |
EUR 176 |
|
|
 Gly-Pro-AMC *CAS 115035-46-6* |
|
13450 |
AAT Bioquest |
5 mg |
EUR 132 |
|
|
 Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence* |
|
13456 |
AAT Bioquest |
100 tests |
EUR 306 |
|
|
After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.
