Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.

Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.

Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.

In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.

Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.

In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).

Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.

FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).

S-Adenosyl-L-homocysteine (SAH) ELISA Kit

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S-Adenosyl-L-homocysteine (SAH) ELISA Kit

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Aflatoxin M2 (AFM2) ELISA Kit

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abx159326-100ul 100 ul
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Alpha Tubulin Acetyltransferase 1 (ATAT1) Antibody

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EUR 356.00
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AICAR Formyltransferase/IMP Cyclohydrolase Bifunctional Enzyme (ATIC) Antibody

abx159338-100ul 100 ul
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Beclin 1 (BECN1) Antibody

abx159345-100ul 100 ul
EUR 467.00
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BRCA1 Associated Protein (BRAP) Antibody

abx159350-100ul 100 ul
EUR 467.00
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Calumenin (CALU) Antibody

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EUR 467.00
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abx159354-100ul 100 ul
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Coactivator Associated Arginine Methyltransferase 1 (CARM1) Antibody

abx159355-100ul 100 ul
EUR 467.00
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Carbonyl Reductase 1 (CBR1) Antibody

abx159359-100ul 100 ul
EUR 467.00
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Chromobox Protein Homolog 5 (CBX5) Antibody

abx159363-100ul 100 ul
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Cell Cycle And Apoptosis Regulator 2 (CCAR2) Antibody

abx159366-100ul 100 ul
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Cyclin H (CCNH) Antibody

abx159369-100ul 100 ul
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159370-100ul 100 ul
EUR 356.00
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159371-100ul 100 ul
EUR 356.00
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159372-100ul 100 ul
EUR 356.00
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159373-100ul 100 ul
EUR 356.00
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159374-100ul 100 ul
EUR 439.00
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Monocyte differentiation antigen CD14 (CD14) Antibody

abx159375-100ul 100 ul
EUR 356.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159376-100ul 100 ul
EUR 356.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159377-100ul 100 ul
EUR 356.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159378-100ul 100 ul
EUR 356.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159379-100ul 100 ul
EUR 356.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159380-100ul 100 ul
EUR 439.00
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B-Lymphocyte Antigen CD19 (CD19) Antibody

abx159381-100ul 100 ul
EUR 356.00
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T-cell Surface Antigen CD2 (CD2) Antibody (FITC)

abx159384-100ul 100 ul
EUR 356.00
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Hematopoietic Progenitor Cell Antigen CD34 (CD34) Antibody

abx159385-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159386-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159387-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159388-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159389-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159390-100ul 100 ul
EUR 439.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159391-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159392-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159393-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159394-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159395-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159396-100ul 100 ul
EUR 356.00
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T-cell surface glycoprotein CD3 epsilon chain (CD3E) Antibody

abx159397-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159399-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159400-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159401-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159402-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159403-100ul 100 ul
EUR 439.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159404-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159405-100ul 100 ul
EUR 356.00
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T-cell Surface Glycoprotein CD4 (CD4) Antibody

abx159406-100ul 100 ul
EUR 356.00
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CD4 / CD25 Antibody (FITC / PE)

abx159407-100ul 100 ul
EUR 356.00
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CD4 / CD8 Antibody (FITC / PE)

abx159408-100ul 100 ul
EUR 356.00
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CD4 / CD8 / CD3 Antibody (FITC / PE / PE-Cy5)

abx159409-100ul 100 ul
EUR 356.00
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CD45 / CD14 Antibody (FITC / PE)

abx159410-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159413-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159414-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159415-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159416-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159417-100ul 100 ul
EUR 439.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159418-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159419-100ul 100 ul
EUR 356.00
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T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8A) Antibody

abx159420-100ul 100 ul
EUR 356.00
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Cyclin Dependent Kinase 1 (CDK1) Antibody

abx159423-100ul 100 ul
EUR 467.00
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Cyclin Dependent Kinase 4 (CDK4) Antibody

abx159424-100ul 100 ul
EUR 467.00
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Cyclin Dependent Kinase 5 (CDK5) Antibody

abx159425-100ul 100 ul
EUR 467.00
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Homeobox Protein CDX-2 (CDX2) Antibody

abx159427-100ul 100 ul
EUR 467.00
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Homeobox Protein CDX-2 (CDX2) Antibody

abx159428-100ul 100 ul
EUR 467.00
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Carcinoembryonic Antigen Related Cell Adhesion Molecule 5 (CEACAM5) Antibody

abx159430-100ul 100 ul
EUR 356.00
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Chromodomain Helicase DNA Binding Protein 3 (CHD3) Antibody

abx159431-100ul 100 ul
EUR 467.00
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Chromodomain Helicase DNA Binding Protein 3 (CHD3) Antibody

abx159432-100ul 100 ul
EUR 467.00
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Chromodomain Helicase DNA Binding Protein 4 (CHD4) Antibody

abx159433-100ul 100 ul
EUR 467.00
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Chordin Like 1 (CHRDL1) Antibody

abx159434-100ul 100 ul
EUR 467.00
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Creatine Kinase, Mitochondrial 1A (CKMT1A) Antibody

abx159435-100ul 100 ul
EUR 467.00
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Creatine Kinase, Mitochondrial 2 (CKMT2) Antibody

abx159436-100ul 100 ul
EUR 467.00
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Cytochrome C Oxidase Subunit 4I1 (COX4I1) Antibody

abx159456-100ul 100 ul
EUR 467.00
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CRK Proto-Oncogene, Adaptor Protein (CRK) Antibody

abx159459-100ul 100 ul
EUR 467.00
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CREB Regulated Transcription Coactivator 1 (CRTC1) Antibody

abx159460-100ul 100 ul
EUR 467.00
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Tyrosine-Protein Kinase CSK (CSK) Antibody

abx159461-100ul 100 ul
EUR 467.00
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Catenin Beta 1 (CTNNB1) Antibody

abx159464-100ul 100 ul
EUR 356.00
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Cytochrome P450 Family 17 Subfamily A Member 1 (CYP17A1) Antibody

abx159466-100ul 100 ul
EUR 467.00
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Dynactin Subunit 1 (DCTN1) Antibody

abx159467-100ul 100 ul
EUR 467.00
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Damage Specific DNA Binding Protein 1 (DDB1) Antibody

abx159468-100ul 100 ul
EUR 467.00
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Damage Specific DNA Binding Protein 1 (DDB1) Antibody

abx159469-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Dihydrolipoamide S-Acetyltransferase (DLAT) Antibody

abx159478-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Thiol:Disulfide Interchange Protein DsbA (DSBA) Antibody

abx159479-100ul 100 ul
EUR 467.00
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Eukaryotic Translation Elongation Factor 2 (EEF2) Antibody

abx159481-100ul 100 ul
EUR 467.00
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EGFP / EYFP Antibody

abx159483-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Epithelial Cell Adhesion Molecule (EPCAM) Antibody

abx159488-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Erb-B2 Receptor Tyrosine Kinase 2 (ERBB2) Antibody

abx159491-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

TNF Receptor Superfamily Member 6 / CD95 / TNFRSF6 (FAS) Antibody

abx159494-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

High Affinity Immunoglobulin Gamma Fc Receptor I (FCGR1A) Antibody

abx159497-100ul 100 ul
EUR 356.00
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High Affinity Immunoglobulin Gamma Fc Receptor I (FCGR1A) Antibody

abx159498-100ul 100 ul
EUR 356.00
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Fc Fragment Of IgG Receptor IIIa (FCGR3A) Antibody

abx159500-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

Proto-Oncogene C-Fos (FOS) Antibody

abx159506-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Forkhead Box O1 (FOXO1) Antibody

abx159507-100ul 100 ul
EUR 467.00
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Forkhead Box P3 (FOXP3) Antibody

abx159508-100ul 100 ul
EUR 467.00
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Forkhead Box P3 (FOXP3) Antibody

abx159509-100ul 100 ul
EUR 467.00
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Fyn-Binding Protein 1 (FYB1) Antibody

abx159511-100ul 100 ul
EUR 467.00
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody

abx159515-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

GATA Binding Protein 6 (GATA6) Antibody

abx159516-100ul 100 ul
EUR 467.00
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Growth Factor Receptor Bound Protein 2 (GRB2) Antibody

abx159521-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

General Transcription Factor IIB (GTF2B) Antibody

abx159524-100ul 100 ul
EUR 467.00
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General Transcription Factor IIH Subunit 1 (GTF2H1) Antibody

abx159525-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Histone Deacetylase 3 (HDAC3) Antibody

abx159527-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Histone Deacetylase 4 (HDAC4) Antibody

abx159528-100ul 100 ul
EUR 467.00
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Hexokinase 1 (HK1) Antibody

abx159534-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Hexokinase 2 (HK2) Antibody

abx159535-100ul 100 ul
EUR 467.00
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Heterogeneous Nuclear Ribonucleoprotein U (HNRNPU) Antibody

abx159536-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Heat Shock Protein Family A (Hsp70) Member 2 (HSPA2) Antibody

abx159541-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Heat Shock Protein Family A (Hsp70) Member 4 (HSPA4) Antibody

abx159542-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Heat Shock Protein Family A (Hsp70) Member 5 (HSPA5) Antibody

abx159543-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Heat Shock Protein Family B (Small) Member 1 (HSPB1) Antibody

abx159547-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Intercellular Adhesion Molecule 1 (ICAM1) Antibody

abx159548-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

Intercellular Adhesion Molecule 1 (ICAM1) Antibody

abx159549-100ul 100 ul
EUR 467.00
  • Shipped within 5-10 working days.

IgG1 Antibody (FITC)

abx159554-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

IgG1 Antibody (PE)

abx159555-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

IgG2a Antibody (FITC)

abx159556-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

IgG2a Antibody (PE)

abx159557-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

IgG2b Antibody (FITC)

abx159558-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

IgG2b Antibody (PE)

abx159559-100ul 100 ul
EUR 356.00
  • Shipped within 5-10 working days.

After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.

Diana

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