Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.

Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.

Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.

In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.

Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.

In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).

Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.

FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).

ETHYL VIOLET AZIDE BROTH

E05-105-500g 500 g
EUR 124

EUGONIC AGAR

E05-106-10kg 10 kg Ask for price

EUGONIC AGAR

E05-106-2Kg 2 Kg Ask for price

EUGONIC AGAR

E05-106-500g 500 g Ask for price

ENDO AGAR

E05-110-10kg 10 kg
EUR 2381

ENDO AGAR

E05-110-2Kg 2 Kg
EUR 557

ENDO AGAR

E05-110-500g 500 g
EUR 188

APT AGAR

A01-101-10kg 10 kg
EUR 1173

APT AGAR

A01-101-2Kg 2 Kg
EUR 294

APT AGAR

A01-101-500g 500 g
EUR 116

AGAR, BACTERIOLOGICAL

A01-102-10kg 10 kg
EUR 1684

AGAR, BACTERIOLOGICAL

A01-102-2kg 2kg
EUR 405

AGAR, BACTERIOLOGICAL

A01-102-500g 500 g
EUR 146

AGAR, HIGH GEL

A01-102IHG-10kg 10 kg
EUR 2219

AGAR, HIGH GEL

A01-102IHG-2Kg 2 Kg
EUR 521

AGAR, HIGH GEL

A01-102IHG-500g 500 g
EUR 178

AGAR,NOBLE

A01-102N-10kg 10 kg
EUR 3910

AGAR,NOBLE

A01-102N-2kg 2kg
EUR 889

AGAR,NOBLE

A01-102N-500g 500 g
EUR 278

ALT. THIOGLYCOLLATE MED.

A01-103-10kg 10 kg Ask for price

ALT. THIOGLYCOLLATE MED.

A01-103-2Kg 2 Kg Ask for price

ALT. THIOGLYCOLLATE MED.

A01-103-500g 500 g Ask for price

AMIES BROTH WITH CHARCOAL

A01-104-10kg 10 kg Ask for price

AMIES BROTH WITH CHARCOAL

A01-104-2kg 2kg Ask for price

AMIES BROTH WITH CHARCOAL

A01-104-500g 500 g Ask for price

AMIES BROTH W/O CHARCOAL

A01-105-10kg 10 kg Ask for price

AMIES BROTH W/O CHARCOAL

A01-105-2Kg 2 Kg Ask for price

AMIES BROTH W/O CHARCOAL

A01-105-500g 500 g Ask for price

AZIDE BLOOD AGAR BASE

A01-113-10kg 10 kg
EUR 2532

AZIDE BLOOD AGAR BASE

A01-113-2kg 2kg
EUR 589

AZIDE BLOOD AGAR BASE

A01-113-500g 500 g
EUR 197

AZIDE DEXTROSE BROTH

A01-114-10kg 10 kg
EUR 868

AZIDE DEXTROSE BROTH

A01-114-2Kg 2 Kg
EUR 228

AZIDE DEXTROSE BROTH

A01-114-500g 500 g
EUR 98

ANAEROBIC AGAR

A01-115-10kg 10 kg
EUR 2556

ANAEROBIC AGAR

A01-115-2kg 2kg
EUR 595

ANAEROBIC AGAR

A01-115-500g 500 g
EUR 198

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1034

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 264

A-1 MEDIUM

A01-116-500g 500 g
EUR 108

AGAROSE 25G-125G -500G

A01-118-10kg 10 kg Ask for price

AGAROSE 25G-125G -500G

A01-118-2kg 2kg Ask for price

AGAROSE 25G-125G -500G

A01-118-500g 500 g
EUR 494

IL-2 Receptor ?, Human Recombinant

7100-10
EUR 196

IL-2 Receptor ?, Human Recombinant

7100-50
EUR 637

Human CellExp? IL-4 R?, Human Recombinant

7101-10
EUR 229

Human CellExp? IL-4 R?, Human Recombinant

7101-50
EUR 881

Human CellExp? IL-6 R?, Human Recombinant

7102-10
EUR 207

Human CellExp? IL-6 R?, Human Recombinant

7102-50
EUR 800

IL-9, Rat Recombinant

7103-10
EUR 278

IL-9, Rat Recombinant

7103-50
EUR 1132

IL-12p80, Human Recombinant

7104-10
EUR 392

IL-12p80, Human Recombinant

7104-50
EUR 1675

IL-17A, Mouse Recombinant

7105-10
EUR 196

IL-17A, Mouse Recombinant

7105-50
EUR 675

IL-17B, Human Recombinant

7106-10
EUR 196

IL-17B, Human Recombinant

7106-50
EUR 675

IL-17D, Human Recombinant

7107-10
EUR 196

IL-17D, Human Recombinant

7107-50
EUR 675

Human CellExp? IL-34, Human Recombinant

7108-10
EUR 278

Human CellExp? IL-34, Human Recombinant

7108-50
EUR 1132

IL-36RA, Human Recombinant

7109-10
EUR 196

IL-36RA, Human Recombinant

7109-50
EUR 675

IL-36?, Human Recombinant

7110-10
EUR 278

IL-36?, Human Recombinant

7110-50
EUR 1132

IL-36?, Human Recombinant

7111-10
EUR 278

IL-36?, Human Recombinant

7111-50
EUR 1132

Activin B, Human Recombinant

7112-10
EUR 479

Activin B, Human Recombinant

7112-50
EUR 2094

AITRL, Human Recombinant

7113-10
EUR 251

AITRL, Human Recombinant

7113-50
EUR 936

Amphiregulin, Human Recombinant

7114-10
EUR 147

Amphiregulin, Human Recombinant

7114-50
EUR 376

ANG-1, Human Recombinant

7115-10
EUR 251

ANG-1, Human Recombinant

7115-50
EUR 936

ANG-2, Human Recombinant

7116-10
EUR 251

ANG-2, Human Recombinant

7116-50
EUR 936

APRIL, Human Recombinant

7117-10
EUR 311

APRIL, Human Recombinant

7117-50
EUR 1300

APRIL, Mouse Recombinant

7118-10
EUR 196

APRIL, Mouse Recombinant

7118-50
EUR 675

Betacellulin, Murine Recombinant

7119-10
EUR 196

Betacellulin, Murine Recombinant

7119-50
EUR 675

Cardiotrophin-1, Murine Recombinant

7121-10
EUR 278

Cardiotrophin-1, Murine Recombinant

7121-50
EUR 1132

sCD100/Semaphorin-4D, Human Recombinant

7126-10
EUR 251

sCD100/Semaphorin-4D, Human Recombinant

7126-50
EUR 936

CTGFL/WISP-2, Human Recombinant

7129-10
EUR 234

CTGFL/WISP-2, Human Recombinant

7129-50
EUR 860

Human CellExp? DKK-1, Human Recombinant

7132-10
EUR 278

Human CellExp? DKK-1, Human Recombinant

7132-50
EUR 1132

Human CellExp? sDLL-1, Human Recombinant

7133-10
EUR 207

Human CellExp? sDLL-1, Human Recombinant

7133-50
EUR 675

Human CellExp? sDLL-4, Human Recombinant

7134-10
EUR 207

Human CellExp? sDLL-4, Human Recombinant

7134-50
EUR 675

Eotaxin-2/CCL24, murine recombinant

7137-10
EUR 207

Eotaxin-2/CCL24, murine recombinant

7137-50
EUR 675

Eotaxin-3/CCL26, human recombinant

7138-10
EUR 207

Eotaxin-3/CCL26, human recombinant

7138-50
EUR 675

Human CellExp? Fetuin A/ASHG, Human Recombinant

7142-10
EUR 153

Human CellExp? Fetuin A/ASHG, Human Recombinant

7142-50
EUR 381

Histone H3 Peptide, biotin conjugate

7143-01
EUR 240

Histone H3 Peptide, biotin conjugate

7143-1
EUR 1132

Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-01
EUR 240

Histone H4 Peptide, tetra-acetylated, biotin conjugate

7144-1
EUR 1132

FGF-5, human recombinant

7147-10
EUR 142

FGF-5, human recombinant

7147-50
EUR 359

FGF-6, human recombinant

7148-10
EUR 207

FGF-6, human recombinant

7148-50
EUR 675

FGF-16, human recombinant

7149-10
EUR 207

FGF-16, human recombinant

7149-50
EUR 675

FGF-17, human recombinant

7150-10
EUR 207

FGF-17, human recombinant

7150-50
EUR 675

FGF-23, human recombinant

7151-10
EUR 229

FGF-23, human recombinant

7151-50
EUR 860

GASP-1, human recombinant

7152-10
EUR 229

GASP-1, human recombinant

7152-50
EUR 833

GCP-2 (CXCL6), human recombinant

7153-10
EUR 207

GCP-2 (CXCL6), human recombinant

7153-50
EUR 675

BMP-9/GDF-2, human recombinant

7154-10
EUR 278

BMP-9/GDF-2, human recombinant

7154-50
EUR 1132

BMP-11/GDF-11, human recombinant

7155-10
EUR 240

BMP-11/GDF-11, human recombinant

7155-50
EUR 860

Gremlin-1, human recombinant

7159-10
EUR 142

Gremlin-1, human recombinant

7159-50
EUR 359

ICAM-1, human recombinant

7161-10
EUR 142

ICAM-1, human recombinant

7161-50
EUR 359

IFN-?1, human recombinant

7162-10
EUR 207

IFN-?1, human recombinant

7162-50
EUR 735

IFN-?2, human recombinant

7163-10
EUR 207

IFN-?2, human recombinant

7163-50
EUR 675

IGF-BP2, human recombinant

7165-10
EUR 251

IGF-BP2, human recombinant

7165-50
EUR 936

IGF-BP4, human recombinant

7166-10
EUR 251

IGF-BP4, human recombinant

7166-50
EUR 936

IGF-BP7, human recombinant

7167-10
EUR 207

IGF-BP7, human recombinant

7167-50
EUR 675

MCP-5/CCL12, murine recombinant

7168-10
EUR 207

MCP-5/CCL12, murine recombinant

7168-50
EUR 675

MIP-1?/CCL3, human recombinant

7173-10
EUR 207

MIP-1?/CCL3, human recombinant

7173-50
EUR 675

MIP-1?/CCL9/10, murine recombinant

7174-10
EUR 207

MIP-1?/CCL9/10, murine recombinant

7174-50
EUR 675

MIP-3/CCL23, human recombinant

7175-10
EUR 207

MIP-3/CCL23, human recombinant

7175-50
EUR 675

Nesfatin-1, human recombinant

7178-10
EUR 137

Nesfatin-1, human recombinant

7178-50
EUR 272

Human CellExp? PECAM-1, Human Recombinant

7184-10
EUR 153

Human CellExp? PECAM-1, Human Recombinant

7184-50
EUR 381

PF-4/CXCL4, murine recombinant

7187-10
EUR 207

PF-4/CXCL4, murine recombinant

7187-50
EUR 675

R-Spondin-1, human recombinant

7189-10
EUR 207

R-Spondin-1, human recombinant

7189-50
EUR 675

R-Spondin-2, human recombinant

7190-10
EUR 207

R-Spondin-2, human recombinant

7190-50
EUR 675

R-Spondin-3, human recombinant

7191-10
EUR 207

R-Spondin-3, human recombinant

7191-50
EUR 675

Relaxin-2, human recombinant

7194-10
EUR 207

Relaxin-2, human recombinant

7194-50
EUR 675

Relaxin-3, human recombinant

7195-10
EUR 207

Relaxin-3, human recombinant

7195-50
EUR 675

SDF-1?/CXCL2, rat recombinant

7199-10
EUR 207

SDF-1?/CXCL2, rat recombinant

7199-50
EUR 675

SDF-1?/CXCL2, rat recombinant

7200-10
EUR 207

SDF-1?/CXCL2, rat recombinant

7200-50
EUR 675

Semaphorin 3A, human recombinant

7201-10
EUR 207

After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.

Leave a Reply

Your email address will not be published.