Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.

by Posted on

Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.

Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.

Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.

In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.

Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.

In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).

Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.

FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).

Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
P761-1 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
P761-10 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin, ESC Tested
P761S - Ask for price

HighQC™ Rat Mesenchymal (Fetal) Stem Cell
ABC-SC0008 1 vial Ask for price
Description: Rat Mesenchymal (Fetal) Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

Human Fetal Bone Marrow Mesenchymal Stem Cells
HUXMF-01001 1*10^6/vial
EUR 1200

Fetal Bovine Serum (South Africa). Embryonic Stem Cells tested - 500ml
FB-1003S/500 500ml
EUR 220

Fetal Bovine Serum (South America). Embryonic Stem Cells tested - 100ml
FB-1001S/100 100ml
EUR 56.82

Fetal Bovine Serum (South America). Embryonic Stem Cells tested - 500ml
FB-1001S/500 500ml
EUR 220

Hyclone Human Mesenchymal Stem Cell Screened FBS US Origin 500 mL (SH30070.03M) - EACH
HYC31 EACH
EUR 1301.36

Fetal Bovine Serum (South Africa). Embryonic Stem Cells tested - 50ml
FB-1003S/50 50ml
EUR 45.49

Fetal Bovine Serum (South America). Embryonic Stem Cells tested - 50ml
FB-1001S/50 50ml
EUR 45.49

Complete Medium For Human Fetal Bone Marrow Mesenchymal Stem Cells
HUXMF-90011 500mL
EUR 319

Chondrogenic Differentiation Medium For Human Fetal Bone Marrow Mesenchymal Stem Cells
HUXMX-90041 100mL
EUR 409

Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-90061 500mL
EUR 399

Mesenchymal Stem Cell Medium
C0013-19 RT 500 mL Bottle
EUR 182

Fetal Bovine Serum US Origin
AB20000 500ml bottle
EUR 0.1

Fetal Bovine Serum, (US, Origin)
cAP-55 500ml
EUR 470.25

Fetal Bovine Serum US Origin
AlphaFBS 500ml bottle
EUR 0.1

Mesenchymal Stem Cell Growth Medium
BPG-001 500ml bottle
EUR 0.1

Mesenchymal Stem Cell Growth Medium
PG007 4X 500ml bottle
EUR 0.1

Serum Free Medium For Human Adipose-derived Mesenchymal Stem Cells （Type I）
HUXMD-90061 500mL
EUR 579

HighQC™ Dog Mesenchymal Stem Cell
ABC-SC0052T 1 vial Ask for price
Description: Derived from bone marrow of Beagle dog and cultured as a monolayer for two passages in Mesenchymal Stem Cell Growth Medium.

Fetal Bovine Serum (EU Origin) - 100ml
FB-1280/100 100ml
EUR 43.4

Fetal Bovine Serum (EU Origin) - 500ml
FB-1280/500 500ml
EUR 195.58

HighQC™ Rabbit Mesenchymal Stem Cell
ABC-SC0051T 1 vial Ask for price
Description: Derived from bone marrow of New Zealand white rabbits and cultured as monolayer for two passages in Mesenchymal Stem Cell Growth Medium

Fetal Bovine Serum (EU Origin) - 50ml
FB-1280/50 50ml
EUR 34.21

Mesenchymal Stem Cell Growth Medium (MSCGM)
cAP-35 500ml
EUR 108.9

Human Mesenchymal Stem Cells
SC00A1 500000
EUR 1086

Human Mesenchymal Stem Cells
MBS556452-INQUIRE INQUIRE Ask for price

FBS - Fetal Bovine Serum USDA Origin
FBS002 500 mL
EUR 574.8

Rabbit Mesenchymal Stem Cells
CSC-C1302 One Frozen vial Ask for price

Human Mesenchymal Stem Cell Expansion Media
MR1016 1 bottle
EUR 311.85
Description: Mesenchymal Stem Cell Expansion Media (450mL)

HighQC™ Rat Lung Mesenchymal Stem Cell
ABC-SC0042T 1 vial Ask for price
Description: Rat Lung Primary Mesenchymal Stem Cells are derived from the lung tissues of day-1 Sprague-Dawley Rats. Rat Lung Primary Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rat Lung Primary Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-6 passages by the ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD29 and CD44 positive by flow cytometry.

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6004312-005mg 0.05(mg
EUR 745

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6004312-5x005mg 5x0.05mg
EUR 3195

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6012322-005mg 0.05(mg
EUR 745

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6012322-5x005mg 5x0.05mg
EUR 3195

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6010094-005mg 0.05(mg
EUR 745

Mesenchymal Stem Cell Protein DSCD75 (C8orf55)
MBS6010094-5x005mg 5x0.05mg
EUR 3195

Fetal Bovine Serum, Premium, US Origin
F0600-050 500ml
EUR 789.6

HighQC™ Mouse Adult Mesenchymal Stem Cell
ABC-SC0056 1 vial Ask for price
Description: Mouse Adult Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

HighQC™ Feline Mesenchymal Stem Cell: FMSC
ABC-SC0113T 1 vial Ask for price
Description: Feline Mesenchymal Stem Cells (FMSC) have the potential to maintain multipotency and proliferate extensively in vitro. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, mesenchymal steml cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.

HighQC™ Bone Marrow Mesenchymal Stem Cell
ABC-TC3119 1 vial Ask for price
Description: Bone Marrow Stromal Cells (Mesenchymal Stem Cells) are derived from Bone Marrow-Mononuclear Cells from the first culture passage. Cells are derived from single donor

Rat mesenchymal Stem cell Flow Cytometry Kit
RAXMX-09011 10 tests
EUR 1079

Mesenchymal Stem Cell (MSC) Basal Medium (MSCBM)
cAP-36 500ml
EUR 64.35

MSCGro-Humanized Mesenchymal Stem Cell, complete
SC00B2-500 500 ml
EUR 450

Fetal Bovine Serum, Origin USA - 500 ml
CAFBS-22A each
EUR 605.54

Fetal Bovine Serum, Origin USA - 100 ml
CAFBS-22B each
EUR 136.04

Human mesenchymal Stem cell Flow Cytometry Kit
HUXMX-09011 10 tests
EUR 1359

Mouse mesenchymal Stem cell Flow Cytometry Kit
MUXMX-09011 10 tests
EUR 799

HighQC™ Human Mesenchymal Stem Cell-Adipose
ABC-TC3701 1 vial Ask for price
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSCs can differentiate into chondrocytes or osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-ad from Gentaur Research Laboratories are isolated from human adipose tissue. HMSC-ad are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-ad are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-ad are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-ad are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

HighQC™ Human Mesenchymal Stem Cell-Hepatic
ABC-TC3703 1 vial Ask for price
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. The fetal liver is the source of early hematopoietic cells and considered as a source of pluripotent stem cells. MSCs can differentiate into chondrocytes or osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence, MSC can be cultured to expand their numbers and transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-he from Gentaur Research Laboratories are isolated from human liver. HMSC-he are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-he are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-he are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-he are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

HighQC™ Human Pulmonary Mesenchymal Stem Cell
ABC-TC3778 1 vial Ask for price
Description: Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. This property, in combination with MSC's developmental plasticity, has generated tremendous interest in the potential use of MSC to replace damaged tissues. Fetal lung has been identified as a rich source of MSCs and has been reported that MSCs can differentiate into neural cells in addition to their mesenchymal differentiation potential; enhance the engraftment of human umbilical cord blood-derived CD34 hematopoietic cells in nonobese diabetic-severe combined immunodeficiency mice. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC.HPMSC from Gentaur Research Laboratories are isolated from lung tissue. HPMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HPMSC are characterized by immunofluorescent method with antibodies to CD73, CD90 and CD105. HPMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMSC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

Fetal Bovine Serum (South Africa Origin) - 100ml
FB-1003/100 100ml
EUR 43.4

Fetal Bovine Serum (South Africa Origin) - 500ml
FB-1003/500 500ml
EUR 195.58

Rat Adipose Mesenchymal Stem Cells
CP-R198 5×105 cells/vial
EUR 580
Description: Rat

HighQC™ CD1 Mouse Lung Mesenchymal Stem Cell
ABC-SC0045T 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult CD1 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

Mouse Adipose Mesenchymal Stem Cells
CP-M138 5×105 cells/vial
EUR 610
Description: Mouse

Rat Endosteal Mesenchymal Stem Cells
CP-R161 5×105 cells/vial
EUR 580
Description: Rat

HighQC™ B129 Mouse Lung Mesenchymal Stem Cell
ABC-SC0043T 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult B129 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

HighQC™ Rat Mesenchymal (Adult) Stem Cell
ABC-SC0010 1 vial Ask for price
Description: Rat Adult Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

Rabbit Adipose Mesenchymal Stem Cells
CP-Rb028 5×105 cells/vial
EUR 640
Description: Rabbit

Mesenchymal Stem Cells cells, cryo amp
LOPT-2501 each
EUR 2046.79

Fetal Bovine Serum (EU Origin). Premium - 100ml
FB-1280B/100 100ml
EUR 46.42

Fetal Bovine Serum (EU Origin). Premium - 50ml
FB-1280B/50 50ml
EUR 36.69

Fetal Bovine Serum (EU Origin). Premium - 500ml
FB-1280B/500 500ml
EUR 202.9

Mouse Endosteal Mesenchymal Stem Cells
CP-M171 5×105 cells/vial
EUR 610
Description: Mouse

Mesenchymal Stem Cells - Adipose Derived
SC00AD1 500,000 Cells
EUR 1086

HighQC™ Mouse Bone Marrow Mesenchymal Stem Cell
ABC-SC0054 1 vial Ask for price
Description: Mouse Bone Marrow Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

Rabbit Endosteal Mesenchymal Stem Cells
CP-Rb014 5×105 cells/vial
EUR 610
Description: Rabbit

HighQC™ Human Mesenchymal (Liver) Stem Cell
ABC-SC0081 1 vial Ask for price
Description: Human Mesenchymal (Liver) Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

HighQC™ Monkey Bone Marrow Mesenchymal Stem Cell
ABC-SC0046T 1 vial Ask for price
Description: Cynomolgus Monkey Bone Marrow Mesenchymal Stem Cells are derived from the tibias of Cynomolgus Monkey. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44 and CD29 by flow cytometry.

HighQC™ Canine Bone Marrow Mesenchymal Stem Cell
ABC-SC0048T 1 vial Ask for price
Description: Canine Bone Marrow Mesenchymal Stem Cells are derived from the tibias of beagle dog. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

HighQC™ Rabbit Bone Marrow Mesenchymal Stem Cell
ABC-SC0050T 1 vial Ask for price
Description: Rabbit Bone Marrow Mesenchymal Stem Cells are derived from the tibias of New Zealand Whit Rabbit. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 2-3 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44, CD90 or CD29 by flow cytometry.

Fetal Bovine Serum (South Africa Origin) - 50ml
FB-1003/50 50ml
EUR 34.21

FBS - Qualified USA Origin Fetal Bovine Serum
FBS005 each
EUR 349.2

Mesenchymal Stem Cells - Placental Derived
SC00P1 500,000 cells
EUR 1086

Mesenchymal Stem Cells-Adipose Derived
MBS556462-INQUIRE INQUIRE Ask for price

HighQC™ Human Mesenchymal Stem Cell-Bone Marrow
ABC-TC3702 1 vial Ask for price
Description: Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSCs can differentiate into chondrocytes or osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-bm from Gentaur Research Laboratories are isolated from human bone marrow. HMSC-bm are cryopreserved at passage one culture and delivered frozen. Each vial contains andgt;5 x 10^5 cells in 1 ml volume. HMSC-bm are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-bm are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-bm are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

HighQC™ Porcine Bone Marrow Mesenchymal Stem Cell
ABC-SC0047T 1 vial Ask for price
Description: Porcine Bone Marrow Mesenchymal Stem Cells are derived from porcine tibias. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44 and CD29 by flow cytometry.

HighQC™ Hamster Bone Marrow Mesenchymal Stem Cell
ABC-SC0049T 1 vial Ask for price
Description: Hamster Bone Marrow Mesenchymal Stem Cells are derived from the tibias of Syrian hamster. Cells are grown in tissue culture plates with Gentaur Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells at passage 2-3 are detached and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Cells are tested for expression of markers using antibodies, CD44, CD90 or CD29 by flow cytometry.

HighQC™ Bone Marrow Mesenchymal Stem Cell-Mixed
ABC-TC3120 1 vial Ask for price
Description: Bone Marrow Stromal Cells (Mesenchymal Stem Cells) are derived from Bone Marrow-Mononuclear Cells from the first culture passage. Cells are derived from multiple donors and mixed

Fetal Bovine Serum Bioprocess, US Origin - 500 ml
CAFBS-24A each
EUR 638.48

Fetal Bovine Serum Bioprocess, US Origin - 100 ml
CAFBS-24B each
EUR 142.94

FBS-Standard USA Origin Fetal Bovine Serum
MBS556507-500mL 500mL
EUR 485

FBS-Standard USA Origin Fetal Bovine Serum
MBS556507-5x500mL 5x500mL
EUR 2040

HighQC™ Human Whartons Jelly Mesenchymal Stem Cell
ABC-SC0127T 1 vial Ask for price
Description: Human Whartons Jelly Mesenchymal Stem Cells provide an ideal culture model for the study of multipotent stem cell biology and differentiation processes. HMSC-WJ are isolated from Wharton's Jelly of human umbilical cord and cryopreserved to ensure optimal phenotype and the highest viability and plating efficiency. HMSC-WJ can be expanded in an undifferentiated state for future differentiation to multiple lineages. Human Mesenchymal Stem Cells may be differentiated down the typical mesenchymal lineages, such as adipogenic, chondrogenic, and osteogenic. Human Whartons Jelly Mesenchymal Stem Cells are characterized by flow cytometry to ensure the proper expression of multiple markers of mesenchymal stem cells. They are uniformly positive for CD29, CD44, CD73, CD90, CD105, and CD166. They are uniformly negative for CD14, CD31, CD34, and CD45. Gentaur has developed optimized media to expand the Human Mesenchymal Stem Cell products in the undifferentiated state, as well as optimized media kits for inducing Adipogenesis, Chondrogenesis, and Osteogenesis. Additionally, provides convenient staining kits for staining the fully differentiated cells.

Rat Bone Marrow Mesenchymal Stem Cells
CP-R131 5×105 cells/vial
EUR 580
Description: Rat

Fetal Bovine Serum, Premium, US Origin, Dialyzed
F0602-050 500ml
EUR 1275.6

Innovative Grade US Origin Fetal Bovine Serum
IGFBSER25ML each
EUR 102
Description: Innovative Grade US Origin Fetal Bovine Serum

Innovative Grade US Origin Fetal Bovine Serum
IGFBSER500ml each
EUR 528
Description: Innovative Grade US Origin Fetal Bovine Serum

USDA Research Grade Origin Fetal Bovine Serum
TM999-100 100ml
EUR 425

USDA Research Grade Origin Fetal Bovine Serum
TM999-500 500ml
EUR 1695

Fetal Bovine Serum Heat Inactivated US Origin
AlphaFBS-HI 500ml bottle
EUR 0.1

Mesenchymal Stem Cells-Placental Derived
MBS556468-INQUIRE INQUIRE Ask for price

HighQC™ Dog Adipose-Derived Mesenchymal Stem Cell
ABC-SC0057T 1 vial Ask for price
Description: Derived from inguinal fat tissue of beagle dogs (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium. Cells are cryopreserved at the second passage.

HighQC™ C57BL/6 Mouse Lung Mesenchymal Stem Cell
ABC-SC0044T 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult C57BL/6 mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry. Cells can be expanded 3-8 passages by the ratio of 1: 3 under the cell culture conditions specified by Gentaur.

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760-1 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760-10 - Ask for price

Fetal Bovine Serum (FBS), Premium, 100% US Origin
P760S - Ask for price

HighQC™ Human Pre-Adipocytes Mesenchymal Stem Cell
ABC-SC0040T 1 vial Ask for price
Description: Human Pre-Adipocyte MSCs from a single adult donor. Gentaur human mesenchymal stem cells have been functionally tested and are compatible with our​ MSC Expansion Medium. Our mesenchymal stem cells are andgt;95% positive for the presence of CD29, CD44, CD73, CD90, CD105, CD166 markers by flow cytometry analysis. Our MSCs are andlt;2% positive for the presence of CD14, CD31, CD34, CD45 markers by flow cytometry analysis. All of the cells provided are tested and are negative for HIV-1, HIV-2, Hepatitis B and Hepatitis C as detected by PCR. The cells are confirmed to be negative for mycoplasma and other detectable microbial contamination.

Mouse Bone Marrow Mesenchymal Stem Cells
ABC-TC3916 1 vial Ask for price
Description: Mouse bone marrow mesenchymal stem cells, 6-week ICR mouse

Mouse Bone Marrow Mesenchymal Stem Cells
CP-M131 5×105 cells/vial
EUR 580
Description: Mouse

Human Bone Marrow Mesenchymal Stem Cells
HUXMA-01001 1*10^6/vial
EUR 1030

Human Mesenchymal Stem cells Bone Marrow
ALHE31 1ml frozen Vial
EUR 620

Fetal Bovine Serum, (US, Origin) Heat-Inactivated
cAP-55-HI 500ml
EUR 470.25

HighQC™ Human Placental Derived Mesenchymal Stem Cell
ABC-SC0131T 1 vial Ask for price
Description: As interest grows in the expanding field of stem cell research, areas such as regenerative medicine, tissue engineering and basic stem cell biology necessitate a reliable cost-effective cell-based tool for use in discovery and design projects. Adipose-derived adult stem cells and their unique differentiation properties can be utilized in any number of research applications and offer decided benefits over similar multipotent cell types.Adipose tissue is typically in greater supply enabling ample sourcing for isolation and production of adult stem cells – at a fraction of the cost for bone marrow-derived counterparts while maintaining similar markers and characteristics. Adult stem cells offer another more subtle benefit allowing scientists to circumvent the ethical issues normally associated with employing embryonic stem cells in biomedical research.Gentaur adult stem cells are available with optimized media formulations to maximize growth, survival and differentiation in culture. All cells have been screened negative for HIV-1, HIV-2, HTLC-1, HTLV-2, Hep-B, Hep-C, and mycoplama.

Rabbit Bone Marrow Mesenchymal Stem Cells
ABC-TC4058 1 vial Ask for price
Description: Rabbit bone marrow mesenchymal stem cells, 5-week rabbit

Canine Bone Marrow Mesenchymal Stem Cells
CAXMX-01001 1*10^6/vial
EUR 999

Rabbit Bone Marrow Mesenchymal Stem Cells
CP-Rb007 5×105 cells/vial
EUR 610
Description: Rabbit

Rabbit Bone Marrow Mesenchymal Stem Cells
RBXMX-01001 1*10^6/vial
EUR 649

Mesenchymal Stem Cells, Mouse, Basal Medium
MBS653076-500mL 500mL
EUR 420

Mesenchymal Stem Cells, Mouse, Basal Medium
MBS653076-5x500mL 5x500mL
EUR 1740

HighQC™ Rabbit Adipose-Derived Mesenchymal Stem Cell
ABC-SC0056T 1 vial Ask for price
Description: Derived from the inguinal fat tissue of New Zealand white rabbits (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium. Cells are cryopreserved at the second passage.

Mesenchymal Stem Cells - Bone Marrow Derived
SC00BM1 500,000 cells
EUR 1086

CD-1 Mouse Lung Mesenchymal Stem Cells
ABC-TC3293 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mesenchymal Stem Cells, MSCGM, T-150 FSK
LOPT-2501T150 each Ask for price

Human Umbilical Cord Mesenchymal Stem Cells
HUXUC-01001 1*10^6/vial
EUR 1159

Human iPSC-derived Mesenchymal Stem Cells
CSC-00205L One Frozen vial Ask for price

HighQC™ Rat Mesenchymal (Bone Marrow) Stem Cell
ABC-SC0009 1 vial Ask for price
Description: Rat Bone Marrow Mesenchymal Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

SD Rat Bone Marrow Mesenchymal Stem Cells
RASMX-01001 1*10^6/vial
EUR 479

Mesenchymal Stem Cells, Mouse, Freezing Medium
MBS653422-50mL 50mL
EUR 435

Mesenchymal Stem Cells, Mouse, Freezing Medium
MBS653422-5x50mL 5x50mL
EUR 1740

Mesenchymal Stem Cells-Bone Marrow Derived
MBS556467-INQUIRE INQUIRE Ask for price

C57BL/6 Mouse Lung Mesenchymal Stem Cells
ABC-TC3213 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

HighQC™ Human Bone Marrow Derived Mesenchymal Stem Cell
ABC-SC0138 1 vial Ask for price
Description: Human Bone Marrow Derived Mesenchymal Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

F344 Rat Bone Marrow Mesenchymal Stem Cells
RAFMX-01001 1*10^6/vial
EUR 479

Human Adipose-Derived Mesenchymal Stem Cells
CR1004-500 1 vial
EUR 725
Description: Cryopreserved vial of human adipose-derived mesenchymal stem cells (500,000 cells)

Human Adipose-derived Mesenchymal Stem Cells
HUXMD-01001 1*10^6/vial
EUR 1079

HighQC™ Human Mesenchymal Stem Cell-Expressing OCT3/4
ABC-SC0032T 1 vial Ask for price
Description: It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent.Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms.Optimizing your hMSC Cultures: 1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..2) Use of our MSCGroTM media is highly recommended.3) Subtrate-We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.

FBS Xtra, Fetal Bovine Serum, Origin USA - 500 ml
CAFBS-26A each
EUR 476.18

FBS Xtra, Fetal Bovine Serum, Origin USA - 100 ml
CAFBS-26B each
EUR 106.14

Exosome derived from human preadipocyte (Mesenchymal Stem Cell)
Exo-SC01-2 Each
EUR 2200.65
Description: Exosome

CD1 Mouse Bone Marrow Mesenchymal Stem Cells
CSC-00800L One Frozen vial Ask for price

Canine Adipose-derived Mesenchymal Stem Cells
CAXMD-01001 1*10^6/vial
EUR 1200

Rabbit Adipose-derived Mesenchymal Stem Cells
RBXMD-01001 1*10^6/vial
EUR 799

HighQC™ RFP-Human Adipose Derived Mesenchymal Stem Cell
ABC-FC0047 1 vial Ask for price
Description: HAdMSCs are isolated from lipoaspirate derived from white adipose tissue and demonstrated with spindle-shaped-, and fibroblast-like cells. RFP-HAdMSCs are puromycin resistant HAdMSCs after trandsfected with lentiviruses expressing RFP under the control of CMV protomer. Each lot of RFP-HAdMSCs is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)), when cultured following the detailed protocol described below.

Wistar Rat Bone Marrow Mesenchymal Stem Cells
RAWMX-01001 1*10^6/vial
EUR 479

HighQC™ Wistar Rat Adipose-Derived Mesenchymal Stem Cell
ABC-SC0055T 1 vial Ask for price
Description: Derived from fat tissue of strain Wistar rats (SPF) and cultured as a monolayer for two passages in Adipose-Derived Mesenchymal Stem Cell Growth Medium.Cells are cryopreserved at the second passage.

Exosome derived from hTERT-immortalized Mesenchymal Stem Cell
Exo-SC03 Each
EUR 2556.8
Description: Exosome

Fetal Bovine Serum (EU Origin). Heat Inactivated - 100ml
FB-1280H/100 100ml
EUR 46.42

Fetal Bovine Serum (EU Origin). Heat Inactivated - 50ml
FB-1280H/50 50ml
EUR 37.29

Fetal Bovine Serum (EU Origin). Heat Inactivated - 500ml
FB-1280H/500 500ml
EUR 198.61

Rat Primary Bone Marrow Mesenchymal Stem Cells
T4835 5x10^5 cells / 1.0 ml
EUR 925

Hyclone Insect Cell Screened Fetal Bovine Serum US Origin 500 mL (SH30070.03I) - EACH
HYC28 EACH
EUR 1301.36

Human Mesenchymal Stem Cell (MSC) Nucleofector Kit, 25 reactions
LOVPE-1001 each
EUR 630.82

Human Mesenchymal Stem Cell (MSC) Nucleofector Kit, 100 reactions
LOVVPE-1001 each Ask for price

Fetal Bovine Serum (EU Origin). Charcoal Stripped - 100ml
FB-1280F/100 100ml
EUR 56.6

Fetal Bovine Serum (EU Origin). Charcoal Stripped - 50ml
FB-1280F/50 50ml
EUR 37.9

Fetal Bovine Serum (EU Origin). Charcoal Stripped - 500ml
FB-1280F/500 500ml
EUR 242

Fetal Bovine Serum (EU Origin). Tetracycline free - 100ml
FB-1280T/100 100ml
EUR 56.82

Fetal Bovine Serum (EU Origin). Tetracycline free - 50ml
FB-1280T/50 50ml
EUR 45.49

Fetal Bovine Serum (EU Origin). Tetracycline free - 500ml
FB-1280T/500 500ml
EUR 220

HighQC™ Human Mesenchymal (Adipose Derived) Stem Cell
ABC-SC0082 1 vial Ask for price
Description: Human Mesenchymal (Adipose Derived) Stem Cell; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

SD Rat Adipose-derived Mesenchymal Stem Cells
RASMD-01001 1*10^6/vial
EUR 639

Human Mesenchymal Stem Cells Expressing Oct3/4
SC00A6 500000
EUR 1420.8

Human Primary Bone Marrow Mesenchymal Stem Cells
T4019 5x10^5 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells - Bone Marrow Derived
MSC001 500,000+ Cells
EUR 1545.6

Mouse Primary Bone Marrow Mesenchymal Stem Cells
T4535 10^6 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells-hepatic (HMSC-he)
CSC-7755W One Frozen vial Ask for price

Human Mesenchymal Stem Cells-adipose (HMSC-ad)
CSC-7757W One Frozen vial Ask for price

Fetal Bovine Serum, Premium, US Origin , Heat Inactivated
F0601-050 500ml
EUR 837.6

Human Umbilical Cord Blood Mesenchymal Stem Cells
HUXUB-01001 1*10^6/vial
EUR 1159

Diabetic Mouse Bone Marrow Mesenchymal Stem Cells
CSC-00801L One Frozen vial Ask for price

Rat Bone Marrow Mesenchymal Stem Cells With GFP
RBXMX-01101 1*10^6/vial
EUR 1089

Rat Bone Marrow Mesenchymal Stem Cells With RFP
RBXMX-01201 1*10^6/vial
EUR 1089

After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.

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