Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.
Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.
Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.
In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.
Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.
In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).
Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.
FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).
Fetal Bovine Serum, Premium, US Origin |
F0600-050 |
GenDepot |
500ml |
EUR 789.6 |
Chondrogenic Differentiation Medium For Human Fetal Bone Marrow Mesenchymal Stem Cells |
HUXMX-90041 |
Cyagen |
100mL |
EUR 409 |
Serum Free Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90061 |
Cyagen |
500mL |
EUR 399 |
Fetal Bovine Serum, Premium, US Origin, Dialyzed |
F0602-050 |
GenDepot |
500ml |
EUR 1275.6 |
Fetal Bovine Serum (FBS), Premium, 100% US Origin |
P760-1 |
101Bio |
- |
Ask for price |
Fetal Bovine Serum (FBS), Premium, 100% US Origin |
P760-10 |
101Bio |
- |
Ask for price |
Fetal Bovine Serum (FBS), Premium, 100% US Origin |
P760S |
101Bio |
- |
Ask for price |
Serum Free Medium For Human Adipose-derived Mesenchymal Stem Cells (Type I) |
HUXMD-90061 |
Cyagen |
500mL |
EUR 579 |
Human Mesenchymal Stem Cells |
SC00A1 |
Neuromics |
500000 |
EUR 1086 |
Fetal Bovine Serum, Premium, US Origin , Heat Inactivated |
F0601-050 |
GenDepot |
500ml |
EUR 837.6 |
Bovine Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS), BSE-TSE free (Australian Origin), Low endotoxin |
FETB16-N-1 |
Alpha Diagnostics |
1 g |
EUR 270 |
Rat mesenchymal Stem cell Flow Cytometry Kit |
RAXMX-09011 |
Cyagen |
10 tests |
EUR 1079 |
Human mesenchymal Stem cell Flow Cytometry Kit |
HUXMX-09011 |
Cyagen |
10 tests |
EUR 1359 |
Mouse mesenchymal Stem cell Flow Cytometry Kit |
MUXMX-09011 |
Cyagen |
10 tests |
EUR 799 |
Mesenchymal Stem Cells - Adipose Derived |
SC00AD1 |
Neuromics |
500,000 Cells |
EUR 1086 |
MSCGro-Humanized Mesenchymal Stem Cell, complete |
SC00B2-500 |
Neuromics |
500 ml |
EUR 450 |
Mesenchymal Stem Cells - Placental Derived |
SC00P1 |
Neuromics |
500,000 cells |
EUR 1086 |
Human Bone Marrow Mesenchymal Stem Cells |
HUXMA-01001 |
Cyagen |
1*10^6/vial |
EUR 1030 |
Canine Bone Marrow Mesenchymal Stem Cells |
CAXMX-01001 |
Cyagen |
1*10^6/vial |
EUR 999 |
Rabbit Bone Marrow Mesenchymal Stem Cells |
RBXMX-01001 |
Cyagen |
1*10^6/vial |
EUR 649 |
Mesenchymal Stem Cells - Bone Marrow Derived |
SC00BM1 |
Neuromics |
500,000 cells |
EUR 1086 |
SD Rat Bone Marrow Mesenchymal Stem Cells |
RASMX-01001 |
Cyagen |
1*10^6/vial |
EUR 479 |
Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-01001 |
Cyagen |
1*10^6/vial |
EUR 1159 |
F344 Rat Bone Marrow Mesenchymal Stem Cells |
RAFMX-01001 |
Cyagen |
1*10^6/vial |
EUR 479 |
Human Adipose-derived Mesenchymal Stem Cells |
HUXMD-01001 |
Cyagen |
1*10^6/vial |
EUR 1079 |
Wistar Rat Bone Marrow Mesenchymal Stem Cells |
RAWMX-01001 |
Cyagen |
1*10^6/vial |
EUR 479 |
Canine Adipose-derived Mesenchymal Stem Cells |
CAXMD-01001 |
Cyagen |
1*10^6/vial |
EUR 1200 |
Rabbit Adipose-derived Mesenchymal Stem Cells |
RBXMD-01001 |
Cyagen |
1*10^6/vial |
EUR 799 |
FETAL BOVINE SERUM, REGULAR, NEW ZEALAND ORIGIN, GAMMA IRRADIATED, 500 ML |
35-082-CV |
CORNING |
500 mL/pk |
EUR 1296 |
Description: Serum; Serum |
Human Mesenchymal Stem Cells Expressing Oct3/4 |
SC00A6 |
Neuromics |
500000 |
EUR 1420.8 |
SD Rat Adipose-derived Mesenchymal Stem Cells |
RASMD-01001 |
Cyagen |
1*10^6/vial |
EUR 639 |
Human Mesenchymal Stem Cells - Bone Marrow Derived |
MSC001 |
Neuromics |
500,000+ Cells |
EUR 1545.6 |
Rat Bone Marrow Mesenchymal Stem Cells With GFP |
RBXMX-01101 |
Cyagen |
1*10^6/vial |
EUR 1089 |
Rat Bone Marrow Mesenchymal Stem Cells With RFP |
RBXMX-01201 |
Cyagen |
1*10^6/vial |
EUR 1089 |
Human Umbilical Cord Blood Mesenchymal Stem Cells |
HUXUB-01001 |
Cyagen |
1*10^6/vial |
EUR 1159 |
Balb/c Mouse Bone Marrow Mesenchymal Stem Cells |
MUCMX-01001 |
Cyagen |
1*10^6/vial |
EUR 639 |
Human Bone Marrow Mesenchymal Stem Cells With GFP |
HUXMA-01101 |
Cyagen |
1*10^6/vial |
EUR 1479 |
Human Bone Marrow Mesenchymal Stem Cells With RFP |
HUXMA-01201 |
Cyagen |
1*10^6/vial |
EUR 1479 |
C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells |
MUBMX-01001 |
Cyagen |
1*10^6/vial |
EUR 639 |
Wistar Rat Adipose-derived Mesenchymal Stem Cells |
RAWMD-01001 |
Cyagen |
1*10^6/vial |
EUR 639 |
Canine Bone Marrow Mesenchymal Stem Cells With GFP |
CAXMX-01101 |
Cyagen |
1*10^6/vial |
EUR 1229 |
Canine Bone Marrow Mesenchymal Stem Cells With RFP |
CAXMX-01201 |
Cyagen |
1*10^6/vial |
EUR 1229 |
SD Rat Bone Marrow Mesenchymal Stem Cells With GFP |
RASMX-01101 |
Cyagen |
1*10^6/vial |
EUR 929 |
SD Rat Bone Marrow Mesenchymal Stem Cells With RFP |
RASMX-01201 |
Cyagen |
1*10^6/vial |
EUR 929 |
Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7 |
T0352 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human Umbilical Cord Mesenchymal Stem Cells With GFP |
HUXUC-01101 |
Cyagen |
1*10^6/vial |
EUR 1599 |
F344 Rat Bone Marrow Mesenchymal Stem Cells With GFP |
RAFMX-01101 |
Cyagen |
1*10^6/vial |
EUR 929 |
F344 Rat Bone Marrow Mesenchymal Stem Cells With RFP |
RAFMX-01201 |
Cyagen |
1*10^6/vial |
EUR 929 |
Balb/c Mouse Adipose-derived Mesenchymal Stem Cells |
MUCMD-01001 |
Cyagen |
1*10^6/vial |
EUR 649 |
MSCGro-Humanized Mesenchymal Stem Cell Clinical Grade Medium, SF |
SC00B4-500 |
Neuromics |
500 ml |
EUR 434.4 |
Human Mesenchymal Stem Cells-Red Fluorescent-Labeled |
SC00A3 |
Neuromics |
500000 |
EUR 1154.4 |
Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc |
T0169 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras |
T0170 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Wistar Rat Bone Marrow Mesenchymal Stem Cells With GFP |
RAWMX-01101 |
Cyagen |
1*10^6/vial |
EUR 929 |
Wistar Rat Bone Marrow Mesenchymal Stem Cells With RFP |
RAWMX-01201 |
Cyagen |
1*10^6/vial |
EUR 929 |
Human Adipose-derived Mesenchymal Stem Cells With GFP |
HUXMD-01101 |
Cyagen |
1*10^6/vial |
EUR 1479 |
Human Adipose-derived Mesenchymal Stem Cells With RFP |
HUXMD-01201 |
Cyagen |
1*10^6/vial |
EUR 1479 |
C57BL/6 Mouse Adipose-Derived Mesenchymal Stem Cells |
MUBMD-01001 |
Cyagen |
1*10^6/vial |
EUR 649 |
Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40 |
T0167 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human Mesenchymal Stem Cells-Green Fluorescent-Labeled |
SC00A2 |
Neuromics |
500000 |
EUR 1154.4 |
Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT |
T0168 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
SD Rat Adipose-derived Mesenchymal Stem Cells With GFP |
RASMD-01101 |
Cyagen |
1*10^6/vial |
EUR 1239 |
SD Rat Adipose-derived Mesenchymal Stem Cells With RFP |
RASMD-01201 |
Cyagen |
1*10^6/vial |
EUR 1239 |
Immortalized Human Bone Marrow Mesenchymal Stem Cells SV40 |
T0520 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Balb/c Mouse Bone Marrow Mesenchymal Stem Cells With GFP |
MUCMX-01101 |
Cyagen |
1*10^6/vial |
EUR 1079 |
Human Umbilical Cord Blood Mesenchymal Stem Cells With GFP |
HUXUB-01101 |
Cyagen |
1*10^6/vial |
EUR 1599 |
Complete Medium For Rat Bone Marrow Mesenchymal Stem Cells |
RAXMX-90011 |
Cyagen |
500mL |
EUR 289 |
C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With GFP |
MUBMX-01101 |
Cyagen |
1*10^6/vial |
EUR 1079 |
C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells With RFP |
MUBMX-01201 |
Cyagen |
1*10^6/vial |
EUR 1079 |
Complete Medium For Human Bone Marrow Mesenchymal Stem Cells |
HUXMA-90011 |
Cyagen |
500mL |
EUR 319 |
Complete Medium For Mouse Bone Marrow Mesenchymal Stem Cells |
MUXMX-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Canine Bone Marrow Mesenchymal Stem Cells |
CAXMX-90011 |
Cyagen |
500mL |
EUR 319 |
Complete Medium For Monkey Bone Marrow Mesenchymal Stem Cells |
MKXMA-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Rabbit Bone Marrow Mesenchymal Stem Cells |
RBXMX-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90011 |
Cyagen |
500mL |
EUR 319 |
Complete Medium For Rat Adipose-derived Mesenchymal Stem Cells |
RAXMD-90011 |
Cyagen |
500mL |
EUR 289 |
C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP |
MUBMD-01101 |
Cyagen |
1*10^6/vial |
EUR 1239 |
Complete Medium For Canine Adipose-derived Mesenchymal Stem Cells |
CAXMD-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Human Adipose-derived Mesenchymal Stem Cells |
HUXMD-90011 |
Cyagen |
500mL |
EUR 319 |
Complete Medium For Monkey Adipose-derived Mesenchymal Stem Cells |
MKXMD-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Mouse Adipose-derived Mesenchymal Stem Cells |
MUXMD-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Rabbit Adipose-derived Mesenchymal Stem Cells |
RBXMD-90011 |
Cyagen |
500mL |
EUR 289 |
Complete Medium For Human Umbilical Cord Blood Mesenchymal Stem Cells |
HUXUB-90011 |
Cyagen |
500mL |
EUR 319 |
Osteogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells |
RAXMX-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells |
RAXMX-90031 |
Cyagen |
200+100mL |
EUR 359 |
Osteogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells |
HUXMX-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells |
HUXMX-90031 |
Cyagen |
200+100mL |
EUR 359 |
Osteogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells |
MUXMX-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells |
MUXMX-90031 |
Cyagen |
200+100mL |
EUR 359 |
Chondrogenic Differentiation Medium For Rat Bone Marrow Mesenchymal Stem Cells |
RAXMX-90041 |
Cyagen |
100mL |
EUR 409 |
Chondrogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells |
HUXMD-90041 |
Cyagen |
100mL |
EUR 409 |
Hepatogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells |
HUXMX-90101 |
Cyagen |
50+100+200mL |
EUR 759 |
Chondrogenic Differentiation Medium For Mouse Bone Marrow Mesenchymal Stem Cells |
MUXMX-90041 |
Cyagen |
100mL |
EUR 409 |
PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes |
EXOP-140A-1 |
SBI |
50 ug |
EUR 525 |
Mesenchymal Cell Kit |
abx290006-50test |
Abbexa |
50 test |
EUR 2949.6 |
|
Osteogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90031 |
Cyagen |
200+100mL |
EUR 359 |
Osteogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells |
RAXMD-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells |
RAXMD-90031 |
Cyagen |
200+100mL |
EUR 359 |
Osteogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells |
HUXMD-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Human Adipose-derived Mesenchymal Stem Cells |
HUXMD-90031 |
Cyagen |
200+100mL |
EUR 359 |
Chondrogenic Differentiation Medium For Human Umbilical Cord Mesenchymal Stem Cells |
HUXUC-90041 |
Cyagen |
100mL |
EUR 409 |
Osteogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells |
MUXMD-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells |
MUXMD-90031 |
Cyagen |
200+100mL |
EUR 359 |
Chondrogenic Differentiation Medium For Rat Adipose-derived Mesenchymal Stem Cells |
RAXMD-90041 |
Cyagen |
100mL |
EUR 409 |
PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes |
EXOP-145A-1 |
SBI |
50 ug |
EUR 525 |
Osteogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose) |
GUXMX-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose) |
GUXMX-90031 |
Cyagen |
200+100mL |
EUR 359 |
Chondrogenic Differentiation Medium For Bone Marrow Mesenchymal Stem Cells (Multipurpose) |
GUXMX-90041 |
Cyagen |
100mL |
EUR 409 |
Chondrogenic Differentiation Medium For Mouse Adipose-derived Mesenchymal Stem Cells |
MUXMD-90041 |
Cyagen |
100mL |
EUR 409 |
Osteogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose) |
GUXMD-90021 |
Cyagen |
200mL |
EUR 250 |
Adipogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose) |
GUXMD-90031 |
Cyagen |
200+100mL |
EUR 359 |
Chondrogenic Differentiation Medium For Adipose-derived Mesenchymal Stem Cells (Multipurpose) |
GUXMD-90041 |
Cyagen |
100mL |
EUR 409 |
Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested |
CA068-010 |
GenDepot |
100g |
EUR 375.6 |
Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested |
CA068-050 |
GenDepot |
500g |
EUR 1170 |
HQ Fetal Bovine Serum |
abx098128-500ml |
Abbexa |
500 ml |
EUR 895.2 |
|
EQ Fetal Bovine Serum |
abx298025-500ml |
Abbexa |
500 ml |
EUR 1362 |
|
FBS - Fetal Bovine Serum |
FBS001 |
Neuromics |
500 ml |
EUR 654 |
Vimentin (Mesenchymal Cell Marker) (VM452) Antibody |
BNUB0452-50 |
Biotium |
50uL |
EUR 486 |
Description: Primary antibody against Vimentin (Mesenchymal Cell Marker) (VM452), 1mg/mL |
Fetal Bovine Serum, Triple Low |
F0610-050 |
GenDepot |
500ml |
Ask for price |
Fetal Bovine Serum(Best-Quality ) |
FBSAD-01011 |
Cyagen |
100/500mL |
Ask for price |
Fetal Bovine Serum (FBS) [50 ml] |
ABP-MED-FBS0050 |
Allele Biotech |
50ml |
Ask for price |
|
Fetal Bovine Serum (FBS) [500 ml] |
ABP-MED-FBS0500 |
Allele Biotech |
500ml |
Ask for price |
|
Fetal Bovine Serum(Without Exosome ) |
FBSNE-01061 |
Cyagen |
20/50mL |
Ask for price |
Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate) |
RA0338-C.1 |
ScyTek Laboratories |
0.1 ml |
EUR 150 |
Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate) |
RA0338-C.5 |
ScyTek Laboratories |
0.5 ml |
EUR 296.4 |
After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.