Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells.
Erythropoietin receptor (EPORs) are present not only in the precursors of erythrocytes but also in non-hematopoietic cells, including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in the culture medium at EPOR expression and survival of estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells.
Using flow cytometry, we demonstrated that the inclusion of 10% FBS in the medium increased the expression of EPOR and viability of MDA-MB-231 and MCF-7 cells. MDA-MB-231 demonstrated greater EPOR expression of MCF-7 cells, suggesting that the presence of ERs in the cells is associated with poor expression of EPOR.
Culture medium containing 10% FBS also leads to an increase in the number of breast cancer cells enter the synthesis phase of the cell cycle. The study also shows that rHuEPO treatment did not affect the survival of breast cancer cells.
In conclusion, it was shown that the inclusion of FBS in the culture medium EPOR increased expression in breast cancer cells and rHuEPO treatment had no effect on the proliferation of the cancer cells.
Replacement of Fetal Bovine Serum with Bovine Serum Albumin During oocyte maturation and Embryo Culture Does Not Improve Quality After Slow Freezing Blastocyst Cryopreservation.
In this study, four experimental groups were used: fresh embryos, cultured for in vitro maturation and in vitro culture media are equipped with bovine serum albumin (BSA) (fresh BSA) or fetal bovine serum (FBS) (fresh FBS); and two groups of embryos cryopreserved and thawed, produced under the same conditions (BSA frozen and frozen FBS).
Experiment 1 evaluated the effects of a source of protein in embryonic development and in response to cryopreservation. On day 7, half of expanded blastocysts (Bx) of each group cryopreserved and warm and the other half was used as control. After heating, the embryos were incubated in the same conditions for 48 hours, and hatching rates were measured at 24 and 48 hours. Total and the number of apoptotic cells were measured in a subset of Bx after 24 hours. Experiment 2 uses Bx 1 trial to compare the expression KRT8, PLAC8, FOSL1, HSP1A1 and HSPA5 genes at the blastocyst hatches at 24 and 48 hours for all groups.
FBS group showed a higher percentage (p <0.05) embryo (42.8% vs 27.9%) and a higher level of Bx (75.0% vs 63.8%) at day 7, compared with the BSA group. At 24 hours postwarming, fresh FBS group showed the highest hatching rate (p <0.05) compared with other treatments. However, at 48 hours, hatching rates were similar (p> 0.05) between groups: fresh FBS (68.1% ± 23.3%), fresh BSA (70.0% ± 31.0%), frozen FBS (39.2 ± 27.1), and BSA frozen (38.2 ± 23.9).
 ETHYL VIOLET AZIDE BROTH |
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| E05-105-500g | Alphabiosciences | 500 g | EUR 124 |
 EUGONIC AGAR |
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| E05-106-10kg | Alphabiosciences | 10 kg | Ask for price |
EUGONIC AGAR |
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| E05-106-2Kg | Alphabiosciences | 2 Kg | Ask for price |
EUGONIC AGAR |
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 ENDO AGAR |
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| E05-110-10kg | Alphabiosciences | 10 kg | EUR 2381 |
ENDO AGAR |
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| E05-110-2Kg | Alphabiosciences | 2 Kg | EUR 557 |
ENDO AGAR |
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| E05-110-500g | Alphabiosciences | 500 g | EUR 188 |
 APT AGAR |
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| A01-101-10kg | Alphabiosciences | 10 kg | EUR 1173 |
APT AGAR |
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| A01-101-2Kg | Alphabiosciences | 2 Kg | EUR 294 |
APT AGAR |
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| A01-101-500g | Alphabiosciences | 500 g | EUR 116 |
 AGAR, BACTERIOLOGICAL |
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| A01-102-10kg | Alphabiosciences | 10 kg | EUR 1684 |
AGAR, BACTERIOLOGICAL |
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| A01-102-2kg | Alphabiosciences | 2kg | EUR 405 |
AGAR, BACTERIOLOGICAL |
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| A01-102-500g | Alphabiosciences | 500 g | EUR 146 |
 AGAR, HIGH GEL |
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| A01-102IHG-10kg | Alphabiosciences | 10 kg | EUR 2219 |
AGAR, HIGH GEL |
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| A01-102IHG-2Kg | Alphabiosciences | 2 Kg | EUR 521 |
AGAR, HIGH GEL |
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| A01-102IHG-500g | Alphabiosciences | 500 g | EUR 178 |
 AGAR,NOBLE |
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| A01-102N-10kg | Alphabiosciences | 10 kg | EUR 3910 |
AGAR,NOBLE |
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| A01-102N-2kg | Alphabiosciences | 2kg | EUR 889 |
AGAR,NOBLE |
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| A01-102N-500g | Alphabiosciences | 500 g | EUR 278 |
 ALT. THIOGLYCOLLATE MED. |
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| A01-103-10kg | Alphabiosciences | 10 kg | Ask for price |
ALT. THIOGLYCOLLATE MED. |
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| A01-103-2Kg | Alphabiosciences | 2 Kg | Ask for price |
ALT. THIOGLYCOLLATE MED. |
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| A01-103-500g | Alphabiosciences | 500 g | Ask for price |
 AMIES BROTH WITH CHARCOAL |
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 AMIES BROTH W/O CHARCOAL |
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| A01-105-10kg | Alphabiosciences | 10 kg | Ask for price |
AMIES BROTH W/O CHARCOAL |
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AMIES BROTH W/O CHARCOAL |
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| A01-105-500g | Alphabiosciences | 500 g | Ask for price |
 AZIDE BLOOD AGAR BASE |
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| A01-113-10kg | Alphabiosciences | 10 kg | EUR 2532 |
AZIDE BLOOD AGAR BASE |
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| A01-113-2kg | Alphabiosciences | 2kg | EUR 589 |
AZIDE BLOOD AGAR BASE |
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| A01-113-500g | Alphabiosciences | 500 g | EUR 197 |
 AZIDE DEXTROSE BROTH |
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| A01-114-10kg | Alphabiosciences | 10 kg | EUR 868 |
AZIDE DEXTROSE BROTH |
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| A01-114-2Kg | Alphabiosciences | 2 Kg | EUR 228 |
AZIDE DEXTROSE BROTH |
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| A01-114-500g | Alphabiosciences | 500 g | EUR 98 |
 ANAEROBIC AGAR |
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| A01-115-10kg | Alphabiosciences | 10 kg | EUR 2556 |
ANAEROBIC AGAR |
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| A01-115-2kg | Alphabiosciences | 2kg | EUR 595 |
ANAEROBIC AGAR |
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| A01-115-500g | Alphabiosciences | 500 g | EUR 198 |
 A-1 MEDIUM |
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| A01-116-10kg | Alphabiosciences | 10 kg | EUR 1034 |
A-1 MEDIUM |
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| A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 264 |
A-1 MEDIUM |
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| A01-116-500g | Alphabiosciences | 500 g | EUR 108 |
 AGAROSE 25G-125G -500G |
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| A01-118-10kg | Alphabiosciences | 10 kg | Ask for price |
AGAROSE 25G-125G -500G |
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| A01-118-2kg | Alphabiosciences | 2kg | Ask for price |
AGAROSE 25G-125G -500G |
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| A01-118-500g | Alphabiosciences | 500 g | EUR 494 |
 IL-2 Receptor ?, Human Recombinant |
|||
| 7100-10 | Biovision | EUR 196 | |
IL-2 Receptor ?, Human Recombinant |
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| 7100-50 | Biovision | EUR 637 | |
 Human CellExp? IL-4 R?, Human Recombinant |
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| 7101-10 | Biovision | EUR 229 | |
Human CellExp? IL-4 R?, Human Recombinant |
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| 7101-50 | Biovision | EUR 881 | |
 Human CellExp? IL-6 R?, Human Recombinant |
|||
| 7102-10 | Biovision | EUR 207 | |
Human CellExp? IL-6 R?, Human Recombinant |
|||
| 7102-50 | Biovision | EUR 800 | |
 IL-9, Rat Recombinant |
|||
| 7103-10 | Biovision | EUR 278 | |
IL-9, Rat Recombinant |
|||
| 7103-50 | Biovision | EUR 1132 | |
 IL-12p80, Human Recombinant |
|||
| 7104-10 | Biovision | EUR 392 | |
IL-12p80, Human Recombinant |
|||
| 7104-50 | Biovision | EUR 1675 | |
 IL-17A, Mouse Recombinant |
|||
| 7105-10 | Biovision | EUR 196 | |
IL-17A, Mouse Recombinant |
|||
| 7105-50 | Biovision | EUR 675 | |
 IL-17B, Human Recombinant |
|||
| 7106-10 | Biovision | EUR 196 | |
IL-17B, Human Recombinant |
|||
| 7106-50 | Biovision | EUR 675 | |
 IL-17D, Human Recombinant |
|||
| 7107-10 | Biovision | EUR 196 | |
IL-17D, Human Recombinant |
|||
| 7107-50 | Biovision | EUR 675 | |
 Human CellExp? IL-34, Human Recombinant |
|||
| 7108-10 | Biovision | EUR 278 | |
Human CellExp? IL-34, Human Recombinant |
|||
| 7108-50 | Biovision | EUR 1132 | |
 IL-36RA, Human Recombinant |
|||
| 7109-10 | Biovision | EUR 196 | |
IL-36RA, Human Recombinant |
|||
| 7109-50 | Biovision | EUR 675 | |
 IL-36?, Human Recombinant |
|||
| 7110-10 | Biovision | EUR 278 | |
IL-36?, Human Recombinant |
|||
| 7110-50 | Biovision | EUR 1132 | |
IL-36?, Human Recombinant |
|||
| 7111-10 | Biovision | EUR 278 | |
IL-36?, Human Recombinant |
|||
| 7111-50 | Biovision | EUR 1132 | |
 Activin B, Human Recombinant |
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| 7112-10 | Biovision | EUR 479 | |
Activin B, Human Recombinant |
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| 7112-50 | Biovision | EUR 2094 | |
 AITRL, Human Recombinant |
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| 7113-10 | Biovision | EUR 251 | |
AITRL, Human Recombinant |
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| 7113-50 | Biovision | EUR 936 | |
 Amphiregulin, Human Recombinant |
|||
| 7114-10 | Biovision | EUR 147 | |
Amphiregulin, Human Recombinant |
|||
| 7114-50 | Biovision | EUR 376 | |
 ANG-1, Human Recombinant |
|||
| 7115-10 | Biovision | EUR 251 | |
ANG-1, Human Recombinant |
|||
| 7115-50 | Biovision | EUR 936 | |
 ANG-2, Human Recombinant |
|||
| 7116-10 | Biovision | EUR 251 | |
ANG-2, Human Recombinant |
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| 7116-50 | Biovision | EUR 936 | |
 APRIL, Human Recombinant |
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| 7117-10 | Biovision | EUR 311 | |
APRIL, Human Recombinant |
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| 7117-50 | Biovision | EUR 1300 | |
 APRIL, Mouse Recombinant |
|||
| 7118-10 | Biovision | EUR 196 | |
APRIL, Mouse Recombinant |
|||
| 7118-50 | Biovision | EUR 675 | |
 Betacellulin, Murine Recombinant |
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| 7119-10 | Biovision | EUR 196 | |
Betacellulin, Murine Recombinant |
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| 7119-50 | Biovision | EUR 675 | |
 Cardiotrophin-1, Murine Recombinant |
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| 7121-10 | Biovision | EUR 278 | |
Cardiotrophin-1, Murine Recombinant |
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| 7121-50 | Biovision | EUR 1132 | |
 sCD100/Semaphorin-4D, Human Recombinant |
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| 7126-10 | Biovision | EUR 251 | |
sCD100/Semaphorin-4D, Human Recombinant |
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| 7126-50 | Biovision | EUR 936 | |
 CTGFL/WISP-2, Human Recombinant |
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| 7129-10 | Biovision | EUR 234 | |
CTGFL/WISP-2, Human Recombinant |
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| 7129-50 | Biovision | EUR 860 | |
 Human CellExp? DKK-1, Human Recombinant |
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| 7132-10 | Biovision | EUR 278 | |
Human CellExp? DKK-1, Human Recombinant |
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| 7132-50 | Biovision | EUR 1132 | |
 Human CellExp? sDLL-1, Human Recombinant |
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| 7133-10 | Biovision | EUR 207 | |
Human CellExp? sDLL-1, Human Recombinant |
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| 7133-50 | Biovision | EUR 675 | |
 Human CellExp? sDLL-4, Human Recombinant |
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| 7134-10 | Biovision | EUR 207 | |
Human CellExp? sDLL-4, Human Recombinant |
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| 7134-50 | Biovision | EUR 675 | |
 Eotaxin-2/CCL24, murine recombinant |
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| 7137-10 | Biovision | EUR 207 | |
Eotaxin-2/CCL24, murine recombinant |
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| 7137-50 | Biovision | EUR 675 | |
 Eotaxin-3/CCL26, human recombinant |
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| 7138-10 | Biovision | EUR 207 | |
Eotaxin-3/CCL26, human recombinant |
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| 7138-50 | Biovision | EUR 675 | |
 Human CellExp? Fetuin A/ASHG, Human Recombinant |
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| 7142-10 | Biovision | EUR 153 | |
Human CellExp? Fetuin A/ASHG, Human Recombinant |
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| 7142-50 | Biovision | EUR 381 | |
 Histone H3 Peptide, biotin conjugate |
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| 7143-01 | Biovision | EUR 240 | |
Histone H3 Peptide, biotin conjugate |
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| 7143-1 | Biovision | EUR 1132 | |
 Histone H4 Peptide, tetra-acetylated, biotin conjugate |
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| 7144-01 | Biovision | EUR 240 | |
Histone H4 Peptide, tetra-acetylated, biotin conjugate |
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| 7144-1 | Biovision | EUR 1132 | |
 FGF-5, human recombinant |
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| 7147-10 | Biovision | EUR 142 | |
FGF-5, human recombinant |
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| 7147-50 | Biovision | EUR 359 | |
 FGF-6, human recombinant |
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| 7148-10 | Biovision | EUR 207 | |
FGF-6, human recombinant |
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| 7148-50 | Biovision | EUR 675 | |
 FGF-16, human recombinant |
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| 7149-10 | Biovision | EUR 207 | |
FGF-16, human recombinant |
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| 7149-50 | Biovision | EUR 675 | |
 FGF-17, human recombinant |
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| 7150-10 | Biovision | EUR 207 | |
FGF-17, human recombinant |
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| 7150-50 | Biovision | EUR 675 | |
 FGF-23, human recombinant |
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| 7151-10 | Biovision | EUR 229 | |
FGF-23, human recombinant |
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| 7151-50 | Biovision | EUR 860 | |
 GASP-1, human recombinant |
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| 7152-10 | Biovision | EUR 229 | |
GASP-1, human recombinant |
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| 7152-50 | Biovision | EUR 833 | |
, human recombinant) GCP-2 (CXCL6), human recombinant |
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| 7153-10 | Biovision | EUR 207 | |
GCP-2 (CXCL6), human recombinant |
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| 7153-50 | Biovision | EUR 675 | |
 BMP-9/GDF-2, human recombinant |
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| 7154-10 | Biovision | EUR 278 | |
BMP-9/GDF-2, human recombinant |
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| 7154-50 | Biovision | EUR 1132 | |
 BMP-11/GDF-11, human recombinant |
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| 7155-10 | Biovision | EUR 240 | |
BMP-11/GDF-11, human recombinant |
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| 7155-50 | Biovision | EUR 860 | |
 Gremlin-1, human recombinant |
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| 7159-10 | Biovision | EUR 142 | |
Gremlin-1, human recombinant |
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| 7159-50 | Biovision | EUR 359 | |
 ICAM-1, human recombinant |
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| 7161-10 | Biovision | EUR 142 | |
ICAM-1, human recombinant |
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| 7161-50 | Biovision | EUR 359 | |
 IFN-?1, human recombinant |
|||
| 7162-10 | Biovision | EUR 207 | |
IFN-?1, human recombinant |
|||
| 7162-50 | Biovision | EUR 735 | |
 IFN-?2, human recombinant |
|||
| 7163-10 | Biovision | EUR 207 | |
IFN-?2, human recombinant |
|||
| 7163-50 | Biovision | EUR 675 | |
 IGF-BP2, human recombinant |
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| 7165-10 | Biovision | EUR 251 | |
IGF-BP2, human recombinant |
|||
| 7165-50 | Biovision | EUR 936 | |
 IGF-BP4, human recombinant |
|||
| 7166-10 | Biovision | EUR 251 | |
IGF-BP4, human recombinant |
|||
| 7166-50 | Biovision | EUR 936 | |
 IGF-BP7, human recombinant |
|||
| 7167-10 | Biovision | EUR 207 | |
IGF-BP7, human recombinant |
|||
| 7167-50 | Biovision | EUR 675 | |
 MCP-5/CCL12, murine recombinant |
|||
| 7168-10 | Biovision | EUR 207 | |
MCP-5/CCL12, murine recombinant |
|||
| 7168-50 | Biovision | EUR 675 | |
 MIP-1?/CCL3, human recombinant |
|||
| 7173-10 | Biovision | EUR 207 | |
MIP-1?/CCL3, human recombinant |
|||
| 7173-50 | Biovision | EUR 675 | |
 MIP-1?/CCL9/10, murine recombinant |
|||
| 7174-10 | Biovision | EUR 207 | |
MIP-1?/CCL9/10, murine recombinant |
|||
| 7174-50 | Biovision | EUR 675 | |
 MIP-3/CCL23, human recombinant |
|||
| 7175-10 | Biovision | EUR 207 | |
MIP-3/CCL23, human recombinant |
|||
| 7175-50 | Biovision | EUR 675 | |
 Nesfatin-1, human recombinant |
|||
| 7178-10 | Biovision | EUR 137 | |
Nesfatin-1, human recombinant |
|||
| 7178-50 | Biovision | EUR 272 | |
 Human CellExp? PECAM-1, Human Recombinant |
|||
| 7184-10 | Biovision | EUR 153 | |
Human CellExp? PECAM-1, Human Recombinant |
|||
| 7184-50 | Biovision | EUR 381 | |
 PF-4/CXCL4, murine recombinant |
|||
| 7187-10 | Biovision | EUR 207 | |
PF-4/CXCL4, murine recombinant |
|||
| 7187-50 | Biovision | EUR 675 | |
 R-Spondin-1, human recombinant |
|||
| 7189-10 | Biovision | EUR 207 | |
R-Spondin-1, human recombinant |
|||
| 7189-50 | Biovision | EUR 675 | |
 R-Spondin-2, human recombinant |
|||
| 7190-10 | Biovision | EUR 207 | |
R-Spondin-2, human recombinant |
|||
| 7190-50 | Biovision | EUR 675 | |
 R-Spondin-3, human recombinant |
|||
| 7191-10 | Biovision | EUR 207 | |
R-Spondin-3, human recombinant |
|||
| 7191-50 | Biovision | EUR 675 | |
 Relaxin-2, human recombinant |
|||
| 7194-10 | Biovision | EUR 207 | |
Relaxin-2, human recombinant |
|||
| 7194-50 | Biovision | EUR 675 | |
 Relaxin-3, human recombinant |
|||
| 7195-10 | Biovision | EUR 207 | |
Relaxin-3, human recombinant |
|||
| 7195-50 | Biovision | EUR 675 | |
 SDF-1?/CXCL2, rat recombinant |
|||
| 7199-10 | Biovision | EUR 207 | |
SDF-1?/CXCL2, rat recombinant |
|||
| 7199-50 | Biovision | EUR 675 | |
SDF-1?/CXCL2, rat recombinant |
|||
| 7200-10 | Biovision | EUR 207 | |
SDF-1?/CXCL2, rat recombinant |
|||
| 7200-50 | Biovision | EUR 675 | |
 Semaphorin 3A, human recombinant |
|||
| 7201-10 | Biovision | EUR 207 | |
After 24 hours, frozen BSA showed a higher number of cells compared with frozen FBS (p <0.05). PLAC8 gene expression was higher (p <0.05) in BSA fresh embryos compared with frozen embryos FBS at 24 hours. In this study, the replacement of BSA reduce embryonic development, but does not affect the response to cryopreservation. However, an increase in gene regulation PLAC8 showed that embryos cultured in BSA may have a better quality to support further development.
