Lysophosphatidylcholine (lysoPtdCho) produced by phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate the proliferation and apoptosis of smooth muscle cells of blood vessels. We investigated the effect of bovine serum (FBS) concentration of the fetus in the culture medium at lysoPtdChomediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as the activation of extracellular signal-regulated kinase (ERK) 1/2.
In the presence of 1% FBS, HASMC increased survival after treatment lysoPtdCho at 1 and 10 pM but decreased at 25 and 50 pM. However, the increased survival lysoPtdCho HASMC dose-dependent manner in the presence of 10% FBS. Caspase 3/7 activity in HASMCs increased by 25 pM lysoPtdCho in the presence of 1% FBS, but not 10% FBS.
Furthermore, lysoPtdCho at 1 and 10 pM induced ERK1 / 2 phosphorylation in the presence of 1% FBS, but not in 10% FBS. Thus, lysoPtdCho HASMC-mediated apoptosis, proliferation, and ERK1 / 2 activation depends on the concentration of FBS.
Fetal bovine serum attenuation-inducing perfluorooctanoic acid toxicity to human cell lines some through albumin binding.
perfluorooctanoic acid (PFOA), a typical emerging organic pollutants, can interact with serum albumin. However, still characterize the binding of PFOA in serum albumin and to address the role of these interactions related toxic effects. We aimed to characterize the interaction between PFOA and albumin to understand the effects of these interactions on the absorption, distribution, and toxicity of PFOA in human cells.
The results showed that the number of viable cells was significantly enhanced by the addition of fetabl bovine serum (FBS) into the cell culture medium with 300 pM treatment PFOA. PFOA is mainly existed as a complex with FBS, the FBS concentration> 10%, which substantially reduces the absorption efficiency of all cell lines PFOA.
The majority of PFOA accumulates in the cytosol fraction, followed by a nucleus, and mitochondria. Conclusively, our research shows that organic contaminants complexation with protein may reduce their toxicity by reducing cellular uptake.
Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.
High quality screening fetal bovine serum for pig oocyte maturation in vitro.
Fetal bovine serum (FBS) is widely used in cell culture due to the high stability and easy access. It is also used as a substitute pig follicular fluid (PFF) in previous studies.
However, components of FBS is not clear, and the FBS in the culture medium can introduce variations from batch to batch. This study aims to establish an effective method to screen in place PFF FBS in culture media for pig oocytes in vitro. We screened FBS from different sources using fibroblast cells from fetal pigs.
Effects of six FBS samples on the growth of fetal pig fibroblast cells were tested through test frozen cell survival, cell clone formation assay, cell growth curve, and the parts of the cell test activities. Serum best we called GFBS (heat-inactivated FBS, cat 10500-64; .. Gibco) showed similar effects on pig oocyte maturation and development with the PFF and can be used as a good substitute for PFF.
Hamster IgG-Biotin conjugate, isotype control (Syrian)
20003-1-B
Alpha Diagnostics
100 test
EUR 164
Hamster IgG, purified (Armenian, Isotype control)
20003-1AH
Alpha Diagnostics
1 mg
EUR 263
G. Pig IgG Fc-Biotin conjugate (isotype control, non-immune) purified
20004-3-B
Alpha Diagnostics
0.1 mg
EUR 225
G. Pig IgG-Biotin conjugate (isotype control)
20004-B
Alpha Diagnostics
100 ug
EUR 202
Rat IgG F(ab')2 fragment, purified (isotype control)
20005-1-FAB2
Alpha Diagnostics
1 mg
EUR 202
Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified
20005-1-FC-B
Alpha Diagnostics
0.1 mg
EUR 225
Rat IgG1-Biotin conjugate (isotype control)
20005-11-B
Alpha Diagnostics
100 ug
EUR 202
Rat IgG2a-APC conjugate (isotype control)
20005-12-APC
Alpha Diagnostics
25 tests
EUR 202
Rat IgG2a-Biotin conjugate (isotype control)
20005-12-B
Alpha Diagnostics
100 ug
EUR 202
Rat IgG2b-Biotin conjugate (isotype control)
20005-13-B
Alpha Diagnostics
100 ug
EUR 202
Rat IgG2c-Biotin conjugate (isotype control)
20005-14-B
Alpha Diagnostics
100 ug
EUR 225
Rat IgM-Biotin conjugate (isotype control)
20005-21-B
Alpha Diagnostics
100 ug
EUR 225
Rat IgG-Biotin conjugate (isotype control) (Isotype control)
20005-B
Alpha Diagnostics
100 ug
EUR 164
Sheep IgG-Biotin Conjugate (isotype control, non-immune), purified
20006-1-B
Alpha Diagnostics
0.5 mg
EUR 202
Sheep IgM-Biotin Conjugate (non-immune) control, purified
20006-2-B
Alpha Diagnostics
0.1 mg
EUR 225
Sheep IgG Fc-Biotin Conjugate (isotype control, non-immune), purified
20006-4-B
Alpha Diagnostics
0.5 mg
EUR 202
Human IgG-Biotin conjugate (isotype control, non-immune), purified
20007-1-B
Alpha Diagnostics
0.5 mg
EUR 225
Human IgG (>98%, non-immune, control, Liquid @ 10 mg/ml, azide free, bulk size)
20007-1-BL-1
Alpha Diagnostics
10 ml
Ask for price
Human IgG (>98%, non-immune, control, powder, azide free, bulk size)
20007-1-BP-1
Alpha Diagnostics
1 g
EUR 651
Human IgG (>98%, non-immune, control, powder, azide free, bulk size)
20007-1-BP-10
Alpha Diagnostics
10 g
EUR 4313
Human IgG Fab fragment-Biotin Conjugate, purified
20007-1-FAB-B
Alpha Diagnostics
0.1 mg
EUR 202
Human IgG Fab2 fragment-Biotin Conjugate, purified
20007-1-FAB2-B
Alpha Diagnostics
0.1 mg
EUR 202
Human IgG Fc-Biotin conjugate (isotype control, non-immune), purified
20007-1-Fc-B
Alpha Diagnostics
0.1 mg
EUR 164
Human IgG (Fc) fragment, purified (>95%, low endotoxin)
20007-1-FC-LE
Alpha Diagnostics
100 ul
EUR 225
Human IgG (Fc) fragment-Biotin purified (>95%, low endotoxin)
20007-1-FC-LE-B
Alpha Diagnostics
25 ug
EUR 286
Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1
Alpha Diagnostics
1 mg
EUR 164
Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN
Alpha Diagnostics
100 ug
EUR 286
TruStrip RDT Human IgG Rapid test cards, 10/pk
20007-1-RDT
Alpha Diagnostics
1 Pk
EUR 171
TruStrip RDT Human IgG Rapid test cards, 25/pk
20007-1-RDT-25
Alpha Diagnostics
1 Pk
EUR 293
Human IgM, purified (native, isotype control, >96% pure, ELISA Grade)
20007-2
Alpha Diagnostics
1 mg
EUR 164
Human IgM purified (isotype control, >96% pure, ELISA Grade)
20007-2-10
Alpha Diagnostics
10 mg
EUR 895
Human IgM purified (isotype control, >96% pure, ELISA Grade)
20007-2-5
Alpha Diagnostics
5 mg
EUR 529
Human IgM (myeloma, >96%, non-immune, control; bulk size)
20007-2-B-100
Alpha Diagnostics
100 mg
Ask for price
Human IgM (myeloma, >96%, non-immune, control; bulk size)
20007-2-B-1000
Alpha Diagnostics
1000 mg
Ask for price
Human IgM-Biotin conjugate (myeloma, isotype control)
20007-2-MB
Alpha Diagnostics
100 ug
EUR 164
Human IgM (Rheumatoid Factor plasma, >40,000 IU/ml, purifed for ELISA)
20007-2-RF
Alpha Diagnostics
100 ul
EUR 469
Human IgE (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)
20007-3-LE-50
Alpha Diagnostics
100 ug
EUR 408
Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)
20007-3-LE-BTN
Alpha Diagnostics
100 ul
EUR 408
Human IgM Fab mu fragment (myeloma)-Biotin conjugate (isotype control)
20007-4-B
Alpha Diagnostics
100 ug
EUR 225
Human IgG-Fc fragment (97%, low endotoxin, azide free)
20007-F-LE-100
Alpha Diagnostics
100 ug
EUR 164
Human IgG-Fc fragment-Biotin (97%, low endotoxin, azide free)
20007-F-LE-BTN
Alpha Diagnostics
100 ug
EUR 286
Human IgG1-Biotin Conjugate (-ve control)
20007-G1-B
Alpha Diagnostics
100 ug
EUR 164
Recombinant (HEK) Human IgG1 Fc (103Cys/Ser, 99-330aa, His-tag, >95%, low endotoxin)
20007-G1-FC
Alpha Diagnostics
50 ug
EUR 286
Recombinant (HEK) Human IgG2-Fc (257Ser/Ala, 99-327aa, His-tag, >95%, low endotoxin)
20007-G2-FC
Alpha Diagnostics
50 ug
EUR 286
Recombinant (HEK) Human IgG3-Fc (99-377aa, His-tag, >95%, low endotoxin)
20007-G3-FC
Alpha Diagnostics
50 ug
EUR 286
Recombinant (HEK) Human IgG4-Fc (108Ser/Pro, 99-327aa, His-tag, >95%, low endotoxin)
20007-G4-FC
Alpha Diagnostics
50 ug
EUR 286
Mouse IgG (>97%, ELISA/RIA/Diagnostic Grade; -ve mouse viruses & Protein A/G free)
20008-1-WA
Alpha Diagnostics
1 mg
EUR 164
Mouse IgG-Biotin conjugate (isotype control)
20008-B
Alpha Diagnostics
50 Tests
EUR 164
Mouse IgG-Biotin conjugate (isotype control)
20008-BT-1
Alpha Diagnostics
1 mg
EUR 164
Mouse IgG Fab-Biotin conjugate (isotype control)
20008-Fab-B
Alpha Diagnostics
100 ug
EUR 164
Mouse IgG F(ab')2-Biotin conjugate (isotype control)
20008-Fab2-B
Alpha Diagnostics
100 ug
EUR 164
Mouse IgG F(ab')2-FITC conjugate (isotype control)
20008-Fab2-F
Alpha Diagnostics
100 ug
EUR 164
Mouse IgG F(ab')2-HRP conjugate (isotype control)
20008-Fab2-HP
Alpha Diagnostics
100 ug
EUR 164
Mouse IgG F(c)-Biotin conjugate (isotype control)
20008-Fc-B
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG Fab-Biotin Conjugate, purified
20009-1-FAB-B
Alpha Diagnostics
0.1 mg
EUR 202
Recombinant (HEK) Rabbit IgG (Fc) (101-323aa, 185Thr/Asn284Ser, ~33 kda, >95%, Low Endotoxin)
20009-1-R-FC
Alpha Diagnostics
50 ug
EUR 286
Rabbit IgM-Biotin Conjugate (isotype control, non-immune), purified
20009-2-1-B
Alpha Diagnostics
0.1 mg
EUR 225
Rabbit IgA-Biotin Conjugate (isotype control, non-immune), purified
20009-3-1-B
Alpha Diagnostics
0.1 mg
EUR 408
Rabbit IgG (Whole, non-immune)-Biotin conjugate
20009-B
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG F(ab')2-biotin conjugate, isotype control
20009-FAb2-B
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG F(ab')2-FITC conjugate, isotype control
20009-FAb2-F
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG F(ab')2-HRP conjugate, isotype control
20009-FAb2-HP
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG F(ab')2-R-PE conjugate, isotype control
20009-FAb2-PE
Alpha Diagnostics
100 tests
EUR 225
Rabbit IgG F(c)-biotin conjugate, isotype control
20009-Fc-B
Alpha Diagnostics
100 ug
EUR 164
Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-1
Alpha Diagnostics
1 mg
EUR 141
Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-5
Alpha Diagnostics
5 mg
EUR 286
Human Insulin Antibody
20010-05011
AssayPro
150 ug
EUR 217
Human Insulin Antibody (Biotin Conjugate)
20010-05021
AssayPro
150 ug
EUR 276
Chicken IgG-Biotin conjugate (isotype control, non-immune), purified
20010-1-B
Alpha Diagnostics
0.5 mg
EUR 225
Chicken IgM-Biotin conjugate (isotype control, non-immune) purified
20010-2-1-b
Alpha Diagnostics
0.1 mg
EUR 225
Chicken IgG-Fc-Biotin conjugate (isotype control, non-immune), purified
20010-3-B
Alpha Diagnostics
0.1 mg
EUR 225
Human Synaptobrevin-2 Antibody
20011-05011
AssayPro
150 ug
EUR 217
Human Synaptobrevin-2 Antibody (Biotin Conjugate)
20011-05021
AssayPro
150 ug
EUR 276
Human Synaptobrevin-2 AssayLite Antibody (FITC Conjugate)
20011-05041
AssayPro
150 ug
EUR 428
Human Synaptobrevin-2 AssayLite Antibody (RPE Conjugate)
20011-05051
AssayPro
150 ug
EUR 428
Human Synaptobrevin-2 AssayLite Antibody (APC Conjugate)
20011-05061
AssayPro
150 ug
EUR 428
Human Synaptobrevin-2 AssayLite Antibody (PerCP Conjugate)
20011-05071
AssayPro
150 ug
EUR 471
Goat IgG FAB fragment-Biotin conjugate
20011-1-FAB-B
Alpha Diagnostics
100 tests
EUR 225
Goat IgG (Whole, non-immune)-Biotin conjugate
20011-B
Alpha Diagnostics
100 ug
EUR 164
Goat IgG F(ab')2 fragment-Biotin conjugate
20011-FAB2-B
Alpha Diagnostics
100 tests
EUR 202
Goat IgG F(ab')2 fragment-Cy5 conjugate
20011-FAB2-Cy5
Alpha Diagnostics
50 tests
EUR 202
Goat IgG F(ab')2 fragment-FITC conjugate
20011-FAB2-F
Alpha Diagnostics
100 tests
EUR 202
Goat IgG FAB(ab')2 fragment-HRP conjugate
20011-FAB2-HP
Alpha Diagnostics
100 tests
EUR 202
Goat IgG F(ab')2 fragment-R-PE conjugate
20011-FAB2-PE
Alpha Diagnostics
50 tests
EUR 202
Goat IgG (fc)-Biotin conjugate (isotype control)
20011-Fc-B
Alpha Diagnostics
100 ug
EUR 164
E. coli GroEL Antibody
20012-05011
AssayPro
150 ug
EUR 217
E. coli GroEL Antibody (Biotin Conjugate)
20012-05021
AssayPro
150 ug
EUR 276
E. coli GroEL AssayLite Antibody (FITC Conjugate)
20012-05041
AssayPro
150 ug
EUR 428
E. coli GroEL AssayLite Antibody (RPE Conjugate)
20012-05051
AssayPro
150 ug
EUR 428
E. coli GroEL AssayLite Antibody (APC Conjugate)
20012-05061
AssayPro
150 ug
EUR 428
E. coli GroEL AssayLite Antibody (PerCP Conjugate)
20012-05071
AssayPro
150 ug
EUR 471
Monkey IgG (Rhesus, Whole, non-immune)-Biotin conjugate
20012-B
Alpha Diagnostics
100 ug
EUR 202
Chimpanzee IgG (non-immune)-Biotin conjugate
20012-Ch-B
Alpha Diagnostics
100 ug
EUR 286
CF488A F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL
20013
Biotium
0.25 mL
EUR 190
Description: Minimum order quantity: 1 unit of 0.25 mL
Human Interferon-gamma (IFN-gamma) Antibody
20013-05011
AssayPro
150 ug
EUR 217
Human Interferon-gamma (IFN-gamma) Antibody (Biotin Conjugate)
20013-05021
AssayPro
150 ug
EUR 276
Human Interferon-gamma (IFN-gamma) AssayLite Antibody (FITC Conjugate)
20013-05041
AssayPro
150 ug
EUR 428
Human Interferon-gamma (IFN-gamma) AssayLite Antibody (RPE Conjugate)
20013-05051
AssayPro
150 ug
EUR 428
Human Interferon-gamma (IFN-gamma) AssayLite Antibody (APC Conjugate)
20013-05061
AssayPro
150 ug
EUR 428
Human Interferon-gamma (IFN-gamma) AssayLite Antibody (PerCP Conjugate)
20013-05071
AssayPro
150 ug
EUR 471
Monkey Serum IgM-biotin conjugate (Rhesus, non-immune, isotype control), purified
20013-1-B
Alpha Diagnostics
25 ug
EUR 225
Annexin V, Recombinant
20014
AAT Bioquest
100 ug
EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200
Annexin V, Recombinant
20015
AAT Bioquest
1 mg
EUR 702
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200
Donkey IgG-Biotin conjugate (non-immune, isotype control) purified
20015-1-B
Alpha Diagnostics
0.5 mg
EUR 225
Donkey IgM-Biotin conjugate (unlabeled, non-immune, isotype control) purified
20015-2-1-B
Alpha Diagnostics
0.1 mg
EUR 225
Donkey IgG (Whole, non-immune)-Biotin conjugate
20015-B
Alpha Diagnostics
100 ug
EUR 164
Dog IgG-Biotin Conjugate (isotype control, non-immune), purified
20016-1-B
Alpha Diagnostics
0.1 mg
EUR 225
Dog IgG F(ab')2 fragment (isotype control, non-immune) purified
20016-1-FAB2
Alpha Diagnostics
1 mg
EUR 164
Dog IgG Fc-Biotin Conjugate (isotype control, non-immune), purified
20016-FC-B
Alpha Diagnostics
0.1 mg
EUR 225
Pig IgG-Biotin conjugate (swine non-immune, isotype control), purified
20017-1-B
Alpha Diagnostics
0.1 mg
EUR 263
Pig IgM-Biotin (swine non-immune, isotype control), purified
20017-2-1-B
Alpha Diagnostics
0.1 mg
EUR 263
Pig IgG Fc-Biotin conjugate (swine non-immune, isotype control), purified
20017-3-B
Alpha Diagnostics
0.1 mg
EUR 263
Monkey Serum IgM-biotin conjugate (Cyno., non-immune, isotype control), purified
20018-1-B
Alpha Diagnostics
100 ug
EUR 263
Monkey IgG (Cynomolgous, non-immune)-Biotin conjugate
20018-B
Alpha Diagnostics
100 ug
EUR 202
These results suggest that fetal fibroblast cell culture test pigs can be used as a valuable method for FBS screen for pig oocyte maturation and embryo development in vitro.