Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

Lysophosphatidylcholine (lysoPtdCho) produced by phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate the proliferation and apoptosis of smooth muscle cells of blood vessels. We investigated the effect of bovine serum (FBS) concentration of the fetus in the culture medium at lysoPtdChomediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as the activation of extracellular signal-regulated kinase (ERK) 1/2.

In the presence of 1% FBS, HASMC increased survival after treatment lysoPtdCho at 1 and 10 pM but decreased at 25 and 50 pM. However, the increased survival lysoPtdCho HASMC dose-dependent manner in the presence of 10% FBS. Caspase 3/7 activity in HASMCs increased by 25 pM lysoPtdCho in the presence of 1% FBS, but not 10% FBS.

Furthermore, lysoPtdCho at 1 and 10 pM induced ERK1 / 2 phosphorylation in the presence of 1% FBS, but not in 10% FBS. Thus, lysoPtdCho HASMC-mediated apoptosis, proliferation, and ERK1 / 2 activation depends on the concentration of FBS.

Fetal bovine serum attenuation-inducing perfluorooctanoic acid toxicity to human cell lines some through albumin binding.

perfluorooctanoic acid (PFOA), a typical emerging organic pollutants, can interact with serum albumin. However, still characterize the binding of PFOA in serum albumin and to address the role of these interactions related toxic effects. We aimed to characterize the interaction between PFOA and albumin to understand the effects of these interactions on the absorption, distribution, and toxicity of PFOA in human cells.

The results showed that the number of viable cells was significantly enhanced by the addition of fetabl bovine serum (FBS) into the cell culture medium with 300 pM treatment PFOA. PFOA is mainly existed as a complex with FBS, the FBS concentration> 10%, which substantially reduces the absorption efficiency of all cell lines PFOA.

The majority of PFOA accumulates in the cytosol fraction, followed by a nucleus, and mitochondria. Conclusively, our research shows that organic contaminants complexation with protein may reduce their toxicity by reducing cellular uptake.

High quality screening fetal bovine serum for pig oocyte maturation in vitro.

Fetal bovine serum (FBS) is widely used in cell culture due to the high stability and easy access. It is also used as a substitute pig follicular fluid (PFF) in previous studies.

However, components of FBS is not clear, and the FBS in the culture medium can introduce variations from batch to batch. This study aims to establish an effective method to screen in place PFF FBS in culture media for pig oocytes in vitro. We screened FBS from different sources using fibroblast cells from fetal pigs.

Effects of six FBS samples on the growth of fetal pig fibroblast cells were tested through test frozen cell survival, cell clone formation assay, cell growth curve, and the parts of the cell test activities. Serum best we called GFBS (heat-inactivated FBS, cat 10500-64; .. Gibco) showed similar effects on pig oocyte maturation and development with the PFF and can be used as a good substitute for PFF.

Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0050	Equitech	50 ml	EUR 285.48

Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0100	Equitech	100 ml	EUR 388.44

Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0500	Equitech	500 ml	EUR 751.92

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBMDY30-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBMDY30-0500	Equitech	500 ml	Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBUDY30-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBUDY30-0500	Equitech	500 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0050	Equitech	50 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0500	Equitech	500 ml	Ask for price

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0100	Equitech	100 ml	EUR 308.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0500	Equitech	500 ml	EUR 780

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0100	Equitech	100 ml	EUR 354.12

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0500	Equitech	500 ml	EUR 900.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0050	Equitech	50 ml	Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0500	Equitech	500 ml	Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0050	Equitech	50 ml	EUR 258.96

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0100	Equitech	100 ml	EUR 291.72

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0500	Equitech	500 ml	EUR 659.88

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0050	Equitech	50 ml	EUR 293.28

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0100	Equitech	100 ml	EUR 354.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0500	Equitech	500 ml	EUR 759.72

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered US Origin
SLS1154	Scientific Laboratory Supplies	500ML	EUR 1120.2

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated
SFBMDY32-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated
SFBMDY32-0500	Equitech	500 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0050	Equitech	50 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0100	Equitech	100 ml	Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0500	Equitech	500 ml	Ask for price

500ML LSG Fetal Bovine Serum (FBS) -Sterile Filtered - Heat Inactivated 500ml
SLS1150	Scientific Laboratory Supplies	500ML	EUR 415.2

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Gamma Irradiated 500ml
SLS1152	Scientific Laboratory Supplies	500ML	EUR 534

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0100	Equitech	100 ml	EUR 330.72

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0500	Equitech	500 ml	EUR 787.8

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0100	Equitech	100 ml	EUR 360.36

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0500	Equitech	500 ml	EUR 907.92

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Heat Inactivated US Origin
SLS1156	Scientific Laboratory Supplies	500ML	EUR 1096.7

Sterile filtered bovine serum, 0.2 micron
SBSU30-0100	Equitech	100 ml	EUR 179.4

Sterile filtered bovine serum, 0.2 micron
SBSU30-0500	Equitech	500 ml	EUR 205.92

Sterile filtered bovine serum, 0.2 micron, heat-inactivated
SBSU32-0100	Equitech	100 ml	EUR 188.76

Sterile filtered bovine serum, 0.2 micron, heat-inactivated
SBSU32-0500	Equitech	500 ml	EUR 209.04

Calf Serum (Sterile Filtered)
88-NC10	Fitzgerald	100 ml	EUR 150
Description: Sterile Filtered Calf Serum

Human AB Serum, Male , Sterile Filtered
H0900-010	GenDepot	100 ml	EUR 295.2

Sterile filtered goat serum, 0.2 micro
SG30-0500	Equitech	500 ml	EUR 234

Sterile filtered rat serum, 0.2 micron
SRT30-0050	Equitech	50 ml	EUR 308.88

Sterile filtered rat serum, 0.2 micron
SRT30-0100	Equitech	100 ml	EUR 383.76

Sterile filtered rat serum, 0.2 micron
SRT30-0500	Equitech	500 ml	EUR 652.08

Sterile filtered Cynomolgus monkey serum
SCM30-0025	Equitech	25ml	EUR 723.84

Sterile filtered goat serum, 0.2 micron
SG30-0100	Equitech	100 ml	EUR 202.8

Sterile filtered calf serum, 0.2 micron
SBCU30-0100	Equitech	100 ml	EUR 179.4

Sterile filtered calf serum, 0.2 micron
SBCU30-0500	Equitech	500 ml	EUR 205.92

Sterile filtered mouse serum, 0.2 micron
SM30-0050	Equitech	50 ml	EUR 293.28

Sterile filtered mouse serum, 0.2 micron
SM30-0100	Equitech	100 ml	EUR 402.48

Sterile filtered mouse serum, 0.2 micron
SM30-0500	Equitech	500 ml	EUR 603.72

Sterile filtered mouse serum, 0.2 micron
SM30-3000	Equitech	3 L	Ask for price

Sterile filtered sheep serum, 0.2 micron
SS30-0100	Equitech	100 ml	EUR 216.84

Sterile filtered sheep serum, 0.2 micron
SS30-0500	Equitech	500 ml	EUR 283.92

Sterile filtered feline serum, 0.2 micron
SF30-0100	Equitech	100 ml	EUR 603.72

Sterile filtered feline serum, 0.2 micron
SF30-0500	Equitech	500 ml	EUR 1404

Sterile filtered equine serum, 0.2 micron
SE30-0100	Equitech	100 ml	EUR 202.8

Sterile filtered equine serum, 0.2 micron
SE30-0500	Equitech	500 ml	EUR 263.64

Sterile filtered donkey serum, 0.2 micron
SD30-0100	Equitech	100 ml	EUR 202.8

Sterile filtered donkey serum, 0.2 micron
SD30-0500	Equitech	500 ml	EUR 263.64

Sterile filtered canine serum, 0.2 micron
SCA30-0050	Equitech	50 ml	EUR 293.28

Sterile filtered canine serum, 0.2 micron
SCA30-0100	Equitech	100 ml	EUR 383.76

Sterile filtered canine serum, 0.2 micron
SCA30-0500	Equitech	500 ml	EUR 723.84

Sterile filtered rabbit serum, 0.2 micron
SR30-0100	Equitech	100 ml	EUR 207.48

Sterile filtered rabbit serum, 0.2 micron
SR30-0500	Equitech	500 ml	EUR 277.68

Sterile filtered porcine serum, 0.2 micron
SP30-0100	Equitech	100 ml	EUR 209.04

Sterile filtered porcine serum, 0.2 micron
SP30-0500	Equitech	500 ml	EUR 249.6

Sterile filtered hamster serum, 0.2 micron
SHA30-0050	Equitech	50 ml	EUR 388.44

Sterile filtered hamster serum, 0.2 micron
SHA30-0100	Equitech	100 ml	EUR 452.4

Sterile filtered hamster serum, 0.2 micron
SHA30-0500	Equitech	500 ml	EUR 865.8

Sterile filtered chicken serum, 0.2 micron
SC30-0100	Equitech	100 ml	EUR 195

Sterile filtered chicken serum, 0.2 micron
SC30-0500	Equitech	500 ml	EUR 234

Sterile filtered guinea pig serum, 0.2 micron
SGP30-0050	Equitech	50 ml	EUR 321.36

Sterile filtered guinea pig serum, 0.2 micron
SGP30-0100	Equitech	100 ml	EUR 375.96

Sterile filtered guinea pig serum, 0.2 micron
SGP30-0500	Equitech	500 ml	EUR 708.24

Sterile filtered newborn calf serum, 0.2 micron
SNBU30-0100	Equitech	100 ml	EUR 195

Sterile filtered newborn calf serum, 0.2 micron
SNBU30-0500	Equitech	500 ml	EUR 240.24

Sterile filtered Rhesus monkey serum, 0.2 micron
SRM30-0025	Equitech	25ml	EUR 723.84

Sterile filtered Rhesus monkey serum, 0.2 micron
SRM30-0050	Equitech	50 ml	EUR 1071.72

Sterile filtered Rhesus monkey serum, 0.2 micron
SRM30-0100	Equitech	100 ml	Ask for price

Sterile filtered Rhesus monkey serum, 0.2 micron
SRM30-0500	Equitech	500 ml	Ask for price

Sterile filtered Cynomolgus monkey serum, 0.2 micron
SCM30-0050	Equitech	50 ml	EUR 1071.72

Sterile filtered Cynomolgus monkey serum, 0.2 micron
SCM30-0100	Equitech	100 ml	Ask for price

Sterile filtered Cynomolgus monkey serum, 0.2 micron
SCM30-0500	Equitech	500 ml	Ask for price

Sterile filtered mouse serum, 0.2 micron, low endotoxin
SM70-0050	Equitech	50 ml	EUR 517.92

Sterile filtered mouse serum, 0.2 micron, low endotoxin
SM70-0100	Equitech	100 ml	EUR 804.96

Sterile filtered mouse serum, 0.2 micron, low endotoxin
SM70-0500	Equitech	500 ml	EUR 2917.2

Sterile filtered mouse serum, 0.2 micron, low endotoxin
SM70-3000	Equitech	3 L	EUR 8255.52

Human AB Serum, Male , Off-The-Clot , Sterile Filtered
H0910-010	GenDepot	100 ml	EUR 360

Sterile filtered rat serum, 0.2 micron, heat-inactivated
SRT32-0050	Equitech	50 ml	EUR 312

Sterile filtered rat serum, 0.2 micron, heat-inactivated
SRT32-0100	Equitech	100 ml	EUR 386.88

Sterile filtered rat serum, 0.2 micron, heat-inactivated
SRT32-0500	Equitech	500 ml	EUR 659.88

Sterile filtered goat serum, 0.2 micron, heat-inactivated
SG32-0100	Equitech	100 ml	EUR 212.16

Sterile filtered goat serum, 0.2 micron, heat-inactivated
SG32-0500	Equitech	500 ml	EUR 246.48

Sterile filtered calf serum, 0.2 micron, heat-inactivated
SBCU32-0100	Equitech	100 ml	EUR 182.52

Sterile filtered calf serum, 0.2 micron, heat-inactivated
SBCU32-0500	Equitech	500 ml	EUR 213.72

Sterile filtered mouse serum, 0.2 micron, heat-inactivated
SM32-0050	Equitech	50 ml	EUR 296.4

Sterile filtered mouse serum, 0.2 micron, heat-inactivated
SM32-0100	Equitech	100 ml	EUR 474.24

Sterile filtered mouse serum, 0.2 micron, heat-inactivated
SM32-0500	Equitech	500 ml	EUR 609.96

Sterile filtered mouse serum, 0.2 micron, heat-inactivated
SM32-3000	Equitech	3 L	Ask for price

Sterile filtered sheep serum, 0.2 micron, heat-inactivated
SS32-0100	Equitech	100 ml	EUR 221.52

Sterile filtered sheep serum, 0.2 micron, heat-inactivated
SS32-0500	Equitech	500 ml	EUR 291.72

Sterile filtered feline serum, 0.2 micron, heat-inactivated
SF32-0100	Equitech	100 ml	EUR 606.84

Sterile filtered feline serum, 0.2 micron, heat-inactivated
SF32-0500	Equitech	500 ml	EUR 1410.24

Sterile filtered chicken serum, 0.2 micron, charcoal stripped
SC31-0050	Equitech	50 ml	EUR 196.56

Sterile filtered chicken serum, 0.2 micron,charcoal stripped
SC31-0100	Equitech	100 ml	EUR 223.08

Sterile filtered chicken serum, 0.2 micron, charcoal stripped
SC31-0500	Equitech	500 ml	EUR 293.28

Sterile filtered equine serum, 0.2 micron, heat-inactivated
SE32-0100	Equitech	100 ml	EUR 205.92

Sterile filtered equine serum, 0.2 micron, heat-inactivated
SE32-0500	Equitech	500 ml	EUR 269.88

Sterile filtered donkey serum, 0.2 micron, heat-inactivated
SD32-0100	Equitech	100 ml	EUR 205.92

Sterile filtered donkey serum, 0.2 micron, heat-inactivated
SD32-0500	Equitech	500 ml	EUR 269.88

Sterile filtered canine serum, 0.2 micron, heat-inactivated
SCA32-0050	Equitech	50 ml	EUR 296.4

Sterile filtered canine serum, 0.2 micron, heat-inactivated
SCA32-0100	Equitech	100 ml	EUR 386.88

Sterile filtered canine serum, 0.2 micron, heat-inactivated
SCA32-0500	Equitech	500 ml	EUR 730.08

Sterile filtered rabbit serum, 0.2 micron, heat-inactivated
SR32-0100	Equitech	100 ml	EUR 210.6

Sterile filtered rabbit serum, 0.2 micron, heat-inactivated
SR32-0500	Equitech	500 ml	EUR 280.8

Sterile filtered porcine serum, 0.2 micron, heat-inactivated
SP32-0100	Equitech	100 ml	EUR 213.72

Sterile filtered porcine serum, 0.2 micron, heat-inactivated
SP32-0500	Equitech	500 ml	EUR 255.84

Sterile filtered hamster serum, 0.2 micron, heat-inactivated
SHA32-0050	Equitech	50 ml	EUR 349.44

Sterile filtered hamster serum, 0.2 micron, heat-inactivated
SHA32-0100	Equitech	100 ml	EUR 455.52

Sterile filtered hamster serum, 0.2 micron, heat-inactivated
SHA32-0500	Equitech	500 ml	EUR 872.04

Sterile filtered chicken serum, 0.2 micron, heat-inactivated
SC32-0100	Equitech	100 ml	EUR 198.12

Sterile filtered chicken serum, 0.2 micron, heat-inactivated
SC32-0500	Equitech	500 ml	EUR 241.8

Sterile filtered bovine plasma with sodium citrate, 0.2 micron
SBPUC35-0100	Equitech	100 ml	EUR 179.4

These results suggest that fetal fibroblast cell culture test pigs can be used as a valuable method for FBS screen for pig oocyte maturation and embryo development in vitro.