Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

Lysophosphatidylcholine (lysoPtdCho) produced by phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate the proliferation and apoptosis of smooth muscle cells of blood vessels. We investigated the effect of bovine serum (FBS) concentration of the fetus in the culture medium at lysoPtdChomediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as the activation of extracellular signal-regulated kinase (ERK) 1/2.

In the presence of 1% FBS, HASMC increased survival after treatment lysoPtdCho at 1 and 10 pM but decreased at 25 and 50 pM. However, the increased survival lysoPtdCho HASMC dose-dependent manner in the presence of 10% FBS. Caspase 3/7 activity in HASMCs increased by 25 pM lysoPtdCho in the presence of 1% FBS, but not 10% FBS.

Furthermore, lysoPtdCho at 1 and 10 pM induced ERK1 / 2 phosphorylation in the presence of 1% FBS, but not in 10% FBS. Thus, lysoPtdCho HASMC-mediated apoptosis, proliferation, and ERK1 / 2 activation depends on the concentration of FBS.

Fetal bovine serum attenuation-inducing perfluorooctanoic acid toxicity to human cell lines some through albumin binding.

perfluorooctanoic acid (PFOA), a typical emerging organic pollutants, can interact with serum albumin. However, still characterize the binding of PFOA in serum albumin and to address the role of these interactions related toxic effects. We aimed to characterize the interaction between PFOA and albumin to understand the effects of these interactions on the absorption, distribution, and toxicity of PFOA in human cells.

The results showed that the number of viable cells was significantly enhanced by the addition of fetabl bovine serum (FBS) into the cell culture medium with 300 pM treatment PFOA. PFOA is mainly existed as a complex with FBS, the FBS concentration> 10%, which substantially reduces the absorption efficiency of all cell lines PFOA.

The majority of PFOA accumulates in the cytosol fraction, followed by a nucleus, and mitochondria. Conclusively, our research shows that organic contaminants complexation with protein may reduce their toxicity by reducing cellular uptake.

Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.
Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

High quality screening fetal bovine serum for pig oocyte maturation in vitro.

Fetal bovine serum (FBS) is widely used in cell culture due to the high stability and easy access. It is also used as a substitute pig follicular fluid (PFF) in previous studies.

However, components of FBS is not clear, and the FBS in the culture medium can introduce variations from batch to batch. This study aims to establish an effective method to screen in place PFF FBS in culture media for pig oocytes in vitro. We screened FBS from different sources using fibroblast cells from fetal pigs.

Effects of six FBS samples on the growth of fetal pig fibroblast cells were tested through test frozen cell survival, cell clone formation assay, cell growth curve, and the parts of the cell test activities. Serum best we called GFBS (heat-inactivated FBS, cat 10500-64; .. Gibco) showed similar effects on pig oocyte maturation and development with the PFF and can be used as a good substitute for PFF.

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 164

Hamster IgG, purified (Armenian, Isotype control)

20003-1AH 1 mg
EUR 263

G. Pig IgG Fc-Biotin conjugate (isotype control, non-immune) purified

20004-3-B 0.1 mg
EUR 225

G. Pig IgG-Biotin conjugate (isotype control)

20004-B 100 ug
EUR 202

Rat IgG F(ab')2 fragment, purified (isotype control)

20005-1-FAB2 1 mg
EUR 202

Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified

20005-1-FC-B 0.1 mg
EUR 225

Rat IgG1-Biotin conjugate (isotype control)

20005-11-B 100 ug
EUR 202

Rat IgG2a-APC conjugate (isotype control)

20005-12-APC 25 tests
EUR 202

Rat IgG2a-Biotin conjugate (isotype control)

20005-12-B 100 ug
EUR 202

Rat IgG2b-Biotin conjugate (isotype control)

20005-13-B 100 ug
EUR 202

Rat IgG2c-Biotin conjugate (isotype control)

20005-14-B 100 ug
EUR 225

Rat IgM-Biotin conjugate (isotype control)

20005-21-B 100 ug
EUR 225

Rat IgG-Agarose conjugate(aff matrix)

20005-AS-1 0.5 ml
EUR 164

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

20005-B 100 ug
EUR 164

Sheep IgG-Biotin Conjugate (isotype control, non-immune), purified

20006-1-B 0.5 mg
EUR 202

Sheep IgM-Biotin Conjugate (non-immune) control, purified

20006-2-B 0.1 mg
EUR 225

Sheep IgG Fc-Biotin Conjugate (isotype control, non-immune), purified

20006-4-B 0.5 mg
EUR 202

Human IgG-Biotin conjugate (isotype control, non-immune), purified

20007-1-B 0.5 mg
EUR 225

Human IgG (>98%, non-immune, control, Liquid @ 10 mg/ml, azide free, bulk size)

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Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-1 1 g
EUR 651

Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-10 10 g
EUR 4313

Human IgG Fab fragment-Biotin Conjugate, purified

20007-1-FAB-B 0.1 mg
EUR 202

Human IgG F(ab')2 fragment, purified

20007-1-FAB2 1 mg
EUR 202

Human IgG Fab2 fragment-Biotin Conjugate, purified

20007-1-FAB2-B 0.1 mg
EUR 202

Human IgG Fc-Biotin conjugate (isotype control, non-immune), purified

20007-1-Fc-B 0.1 mg
EUR 164

Human IgG (Fc) fragment, purified (>95%, low endotoxin)

20007-1-FC-LE 100 ul
EUR 225

Human IgG (Fc) fragment-Biotin purified (>95%, low endotoxin)

20007-1-FC-LE-B 25 ug
EUR 286

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 164

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 286

TruStrip RDT Human IgG Rapid test cards, 10/pk

20007-1-RDT 1 Pk
EUR 171

TruStrip RDT Human IgG Rapid test cards, 25/pk

20007-1-RDT-25 1 Pk
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Human IgM, purified (native, isotype control, >96% pure, ELISA Grade)

20007-2 1 mg
EUR 164

Human IgM purified (isotype control, >96% pure, ELISA Grade)

20007-2-10 10 mg
EUR 895

Human IgM purified (isotype control, >96% pure, ELISA Grade)

20007-2-5 5 mg
EUR 529

Human IgM (myeloma, >96%, non-immune, control; bulk size)

20007-2-B-100 100 mg Ask for price

Human IgM (myeloma, >96%, non-immune, control; bulk size)

20007-2-B-1000 1000 mg Ask for price

Human IgM-Biotin conjugate (myeloma, isotype control)

20007-2-MB 100 ug
EUR 164

Human IgM (Rheumatoid Factor plasma, >40,000 IU/ml, purifed for ELISA)

20007-2-RF 100 ul
EUR 469

Human IgE (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

20007-3-LE-50 100 ug
EUR 408

Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

20007-3-LE-BTN 100 ul
EUR 408

Human IgM Fab mu fragment (myeloma)-Biotin conjugate (isotype control)

20007-4-B 100 ug
EUR 225

Human IgG-Fc fragment (97%, low endotoxin, azide free)

20007-F-LE-100 100 ug
EUR 164

Human IgG-Fc fragment-Biotin (97%, low endotoxin, azide free)

20007-F-LE-BTN 100 ug
EUR 286

Human IgG1-Biotin Conjugate (-ve control)

20007-G1-B 100 ug
EUR 164

Recombinant (HEK) Human IgG1 Fc (103Cys/Ser, 99-330aa, His-tag, >95%, low endotoxin)

20007-G1-FC 50 ug
EUR 286

Human IgG2-Biotin Conjugate

20007-G2-B 100 ug
EUR 164

Recombinant (HEK) Human IgG2-Fc (257Ser/Ala, 99-327aa, His-tag, >95%, low endotoxin)

20007-G2-FC 50 ug
EUR 286

Human IgG3-Biotin Conjugate

20007-G3-B 100 ug
EUR 164

Recombinant (HEK) Human IgG3-Fc (99-377aa, His-tag, >95%, low endotoxin)

20007-G3-FC 50 ug
EUR 286

Human IgG4-Biotin Conjugate

20007-G4-B 100 ug
EUR 164

Recombinant (HEK) Human IgG4-Fc (108Ser/Pro, 99-327aa, His-tag, >95%, low endotoxin)

20007-G4-FC 50 ug
EUR 286

Mouse IgG F(ab')2 fragment, purified

20008-1-Fab2 1 mg
EUR 202

Mouse IgG (>97%, ELISA/RIA/Diagnostic Grade; -ve mouse viruses & Protein A/G free)

20008-1-WA 1 mg
EUR 164

Mouse IgG-Agarose conjugate (aff matrix)

20008-AS-1 0.5 ml
EUR 164

Mouse IgG-Biotin conjugate (isotype control)

20008-B 50 Tests
EUR 164

Mouse IgG-Biotin conjugate (isotype control)

20008-BT-1 1 mg
EUR 164

Mouse IgG Fab-Biotin conjugate (isotype control)

20008-Fab-B 100 ug
EUR 164

Mouse IgG F(ab')2-Biotin conjugate (isotype control)

20008-Fab2-B 100 ug
EUR 164

Mouse IgG F(ab')2-FITC conjugate (isotype control)

20008-Fab2-F 100 ug
EUR 164

Mouse IgG F(ab')2-HRP conjugate (isotype control)

20008-Fab2-HP 100 ug
EUR 164

Mouse IgG F(c)-Biotin conjugate (isotype control)

20008-Fc-B 100 ug
EUR 164

Rabbit IgG Fab-Biotin Conjugate, purified

20009-1-FAB-B 0.1 mg
EUR 202

Rabbit IgG F(ab')2 fragment, purified

20009-1-FAB2 1 mg
EUR 202

Recombinant (HEK) Rabbit IgG (Fc) (101-323aa, 185Thr/Asn284Ser, ~33 kda, >95%, Low Endotoxin)

20009-1-R-FC 50 ug
EUR 286

Rabbit IgM-Biotin Conjugate (isotype control, non-immune), purified

20009-2-1-B 0.1 mg
EUR 225

Rabbit IgA-Biotin Conjugate (isotype control, non-immune), purified

20009-3-1-B 0.1 mg
EUR 408

Rabbit IgG-Agarose conjugate

20009-AS-1 0.5 ml
EUR 164

Rabbit IgG (Whole, non-immune)-Biotin conjugate

20009-B 100 ug
EUR 164

Rabbit IgG-biotinylated (isotype control)

20009-BT-1 1 mg
EUR 286

Rabbit IgG F(ab')2-biotin conjugate, isotype control

20009-FAb2-B 100 ug
EUR 164

Rabbit IgG F(ab')2-FITC conjugate, isotype control

20009-FAb2-F 100 ug
EUR 164

Rabbit IgG F(ab')2-HRP conjugate, isotype control

20009-FAb2-HP 100 ug
EUR 164

Rabbit IgG F(ab')2-R-PE conjugate, isotype control

20009-FAb2-PE 100 tests
EUR 225

Rabbit IgG F(c)-biotin conjugate, isotype control

20009-Fc-B 100 ug
EUR 164

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-1 1 mg
EUR 141

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-5 5 mg
EUR 286

Human Insulin Antibody

20010-05011 150 ug
EUR 217

Human Insulin Antibody (Biotin Conjugate)

20010-05021 150 ug
EUR 276

Chicken IgG-Biotin conjugate (isotype control, non-immune), purified

20010-1-B 0.5 mg
EUR 225

Chicken IgM-Biotin conjugate (isotype control, non-immune) purified

20010-2-1-b 0.1 mg
EUR 225

Chicken IgG-Fc-Biotin conjugate (isotype control, non-immune), purified

20010-3-B 0.1 mg
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Human Synaptobrevin-2 Antibody

20011-05011 150 ug
EUR 217

Human Synaptobrevin-2 Antibody (Biotin Conjugate)

20011-05021 150 ug
EUR 276

Human Synaptobrevin-2 AssayLite Antibody (FITC Conjugate)

20011-05041 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (RPE Conjugate)

20011-05051 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (APC Conjugate)

20011-05061 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (PerCP Conjugate)

20011-05071 150 ug
EUR 471

Goat IgG FAB fragment-Biotin conjugate

20011-1-FAB-B 100 tests
EUR 225

Goat IgG F(ab')2 fragment, purified

20011-1-FAB2 1 mg
EUR 164

Goat IgG-Agarose conjugate(aff matrix)

20011-AS-1 0.5 ml
EUR 164

Goat IgG (Whole, non-immune)-Biotin conjugate

20011-B 100 ug
EUR 164

Goat IgG F(ab')2 fragment-Biotin conjugate

20011-FAB2-B 100 tests
EUR 202

Goat IgG F(ab')2 fragment-Cy5 conjugate

20011-FAB2-Cy5 50 tests
EUR 202

Goat IgG F(ab')2 fragment-FITC conjugate

20011-FAB2-F 100 tests
EUR 202

Goat IgG FAB(ab')2 fragment-HRP conjugate

20011-FAB2-HP 100 tests
EUR 202

Goat IgG F(ab')2 fragment-R-PE conjugate

20011-FAB2-PE 50 tests
EUR 202

Goat IgG (fc)-Biotin conjugate (isotype control)

20011-Fc-B 100 ug
EUR 164

E. coli GroEL Antibody

20012-05011 150 ug
EUR 217

E. coli GroEL Antibody (Biotin Conjugate)

20012-05021 150 ug
EUR 276

E. coli GroEL AssayLite Antibody (FITC Conjugate)

20012-05041 150 ug
EUR 428

E. coli GroEL AssayLite Antibody (RPE Conjugate)

20012-05051 150 ug
EUR 428

E. coli GroEL AssayLite Antibody (APC Conjugate)

20012-05061 150 ug
EUR 428

E. coli GroEL AssayLite Antibody (PerCP Conjugate)

20012-05071 150 ug
EUR 471

Monkey IgG (Rhesus, Whole, non-immune)-Biotin conjugate

20012-B 100 ug
EUR 202

Chimpanzee IgG (non-immune)-Biotin conjugate

20012-Ch-B 100 ug
EUR 286

CF488A F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL

20013 0.25 mL
EUR 190
Description: Minimum order quantity: 1 unit of 0.25 mL

Human Interferon-gamma (IFN-gamma) Antibody

20013-05011 150 ug
EUR 217

Human Interferon-gamma (IFN-gamma) Antibody (Biotin Conjugate)

20013-05021 150 ug
EUR 276

Human Interferon-gamma (IFN-gamma) AssayLite Antibody (FITC Conjugate)

20013-05041 150 ug
EUR 428

Human Interferon-gamma (IFN-gamma) AssayLite Antibody (RPE Conjugate)

20013-05051 150 ug
EUR 428

Human Interferon-gamma (IFN-gamma) AssayLite Antibody (APC Conjugate)

20013-05061 150 ug
EUR 428

Human Interferon-gamma (IFN-gamma) AssayLite Antibody (PerCP Conjugate)

20013-05071 150 ug
EUR 471

Monkey Serum IgM-biotin conjugate (Rhesus, non-immune, isotype control), purified

20013-1-B 25 ug
EUR 225

Annexin V, Recombinant

20014 100 ug
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Annexin V, Recombinant

20015 1 mg
EUR 702
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Donkey IgG-Biotin conjugate (non-immune, isotype control) purified

20015-1-B 0.5 mg
EUR 225

Donkey IgM-Biotin conjugate (unlabeled, non-immune, isotype control) purified

20015-2-1-B 0.1 mg
EUR 225

Donkey IgG (Whole, non-immune)-Biotin conjugate

20015-B 100 ug
EUR 164

Dog IgG-Biotin Conjugate (isotype control, non-immune), purified

20016-1-B 0.1 mg
EUR 225

Dog IgG F(ab')2 fragment (isotype control, non-immune) purified

20016-1-FAB2 1 mg
EUR 164

Dog IgG Fc-Biotin Conjugate (isotype control, non-immune), purified

20016-FC-B 0.1 mg
EUR 225

Pig IgG-Biotin conjugate (swine non-immune, isotype control), purified

20017-1-B 0.1 mg
EUR 263

Pig IgM-Biotin (swine non-immune, isotype control), purified

20017-2-1-B 0.1 mg
EUR 263

Pig IgG Fc-Biotin conjugate (swine non-immune, isotype control), purified

20017-3-B 0.1 mg
EUR 263

Monkey Serum IgM-biotin conjugate (Cyno., non-immune, isotype control), purified

20018-1-B 100 ug
EUR 263

Monkey IgG (Cynomolgous, non-immune)-Biotin conjugate

20018-B 100 ug
EUR 202

These results suggest that fetal fibroblast cell culture test pigs can be used as a valuable method for FBS screen for pig oocyte maturation and embryo development in vitro.

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