Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

Lysophosphatidylcholine (lysoPtdCho) produced by phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate the proliferation and apoptosis of smooth muscle cells of blood vessels. We investigated the effect of bovine serum (FBS) concentration of the fetus in the culture medium at lysoPtdChomediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as the activation of extracellular signal-regulated kinase (ERK) 1/2.

In the presence of 1% FBS, HASMC increased survival after treatment lysoPtdCho at 1 and 10 pM but decreased at 25 and 50 pM. However, the increased survival lysoPtdCho HASMC dose-dependent manner in the presence of 10% FBS. Caspase 3/7 activity in HASMCs increased by 25 pM lysoPtdCho in the presence of 1% FBS, but not 10% FBS.

Furthermore, lysoPtdCho at 1 and 10 pM induced ERK1 / 2 phosphorylation in the presence of 1% FBS, but not in 10% FBS. Thus, lysoPtdCho HASMC-mediated apoptosis, proliferation, and ERK1 / 2 activation depends on the concentration of FBS.

Fetal bovine serum attenuation-inducing perfluorooctanoic acid toxicity to human cell lines some through albumin binding.

perfluorooctanoic acid (PFOA), a typical emerging organic pollutants, can interact with serum albumin. However, still characterize the binding of PFOA in serum albumin and to address the role of these interactions related toxic effects. We aimed to characterize the interaction between PFOA and albumin to understand the effects of these interactions on the absorption, distribution, and toxicity of PFOA in human cells.

The results showed that the number of viable cells was significantly enhanced by the addition of fetabl bovine serum (FBS) into the cell culture medium with 300 pM treatment PFOA. PFOA is mainly existed as a complex with FBS, the FBS concentration> 10%, which substantially reduces the absorption efficiency of all cell lines PFOA.

The majority of PFOA accumulates in the cytosol fraction, followed by a nucleus, and mitochondria. Conclusively, our research shows that organic contaminants complexation with protein may reduce their toxicity by reducing cellular uptake.

Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.
Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.

High quality screening fetal bovine serum for pig oocyte maturation in vitro.

Fetal bovine serum (FBS) is widely used in cell culture due to the high stability and easy access. It is also used as a substitute pig follicular fluid (PFF) in previous studies.

However, components of FBS is not clear, and the FBS in the culture medium can introduce variations from batch to batch. This study aims to establish an effective method to screen in place PFF FBS in culture media for pig oocytes in vitro. We screened FBS from different sources using fibroblast cells from fetal pigs.

Effects of six FBS samples on the growth of fetal pig fibroblast cells were tested through test frozen cell survival, cell clone formation assay, cell growth curve, and the parts of the cell test activities. Serum best we called GFBS (heat-inactivated FBS, cat 10500-64; .. Gibco) showed similar effects on pig oocyte maturation and development with the PFF and can be used as a good substitute for PFF.

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0050 50 ml
EUR 252.72

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0100 100 ml
EUR 283.92

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0500 500 ml
EUR 652.08

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

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EUR 285.48

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

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EUR 388.44

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SFBU30-0500 500 ml
EUR 751.92

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

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Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

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Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered

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Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0100 100 ml
EUR 308.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0500 500 ml
EUR 780

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBU31-0100 100 ml
EUR 354.12

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EUR 900.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

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Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

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Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBA32-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0050 50 ml
EUR 258.96

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0100 100 ml
EUR 291.72

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0500 500 ml
EUR 659.88

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0050 50 ml
EUR 293.28

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0100 100 ml
EUR 354.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0500 500 ml
EUR 759.72

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered US Origin

SLS1154 500ML
EUR 1120.2

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated

SFBMDY32-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated

SFBMDY32-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

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Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

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Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

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Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0100 100 ml
EUR 330.72

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0500 500 ml
EUR 787.8

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0100 100 ml
EUR 360.36

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0500 500 ml
EUR 907.92

500ML LSG Fetal Bovine Serum (FBS) -Sterile Filtered - Heat Inactivated 500ml

SLS1150 500ML
EUR 415.2

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Gamma Irradiated 500ml

SLS1152 500ML
EUR 534

Calf Serum. Iron Supplemented - 500ml

CA-115R/500 500ml
EUR 36.69

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Heat Inactivated US Origin

SLS1156 500ML
EUR 1096.7

Sterile filtered bovine serum, 0.2 micron

SBSU30-0100 100 ml
EUR 179.4

Sterile filtered bovine serum, 0.2 micron

SBSU30-0500 500 ml
EUR 205.92

Sterile filtered bovine serum, 0.2 micron, heat-inactivated

SBSU32-0100 100 ml
EUR 188.76

Sterile filtered bovine serum, 0.2 micron, heat-inactivated

SBSU32-0500 500 ml
EUR 209.04

Calf Serum (Sterile Filtered)

88-NC10 100 ml
EUR 150
Description: Sterile Filtered Calf Serum

Rabbit Serum Sterile Filtered

IRBSERSF500ML each
EUR 1760
Description: Rabbit Serum Sterile Filtered

Human AB Serum, Male , Sterile Filtered

H0900-010 100 ml
EUR 295.2

Sterile filtered goat serum, 0.2 micro

SG30-0500 500 ml
EUR 234

Sterile filtered rat serum, 0.2 micron

SRT30-0050 50 ml
EUR 308.88

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SRT30-0100 100 ml
EUR 383.76

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SRT30-0500 500 ml
EUR 652.08

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SCM30-0025 25ml
EUR 723.84

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SG30-0100 100 ml
EUR 202.8

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EUR 179.4

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SBCU30-0500 500 ml
EUR 205.92

Sterile filtered mouse serum, 0.2 micron

SM30-0050 50 ml
EUR 293.28

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SM30-0100 100 ml
EUR 402.48

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SM30-0500 500 ml
EUR 603.72

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SM30-1000 1 L
EUR 631.25

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SS30-0100 100 ml
EUR 216.84

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SS30-0500 500 ml
EUR 283.92

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SF30-0100 100 ml
EUR 603.72

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SF30-0500 500 ml
EUR 1404

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SE30-0100 100 ml
EUR 202.8

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EUR 263.64

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EUR 202.8

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EUR 263.64

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EUR 293.28

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EUR 383.76

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EUR 723.84

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EUR 431.25

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EUR 207.48

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EUR 277.68

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SP30-0100 100 ml
EUR 209.04

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EUR 249.6

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SHA30-0050 50 ml
EUR 388.44

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EUR 452.4

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EUR 865.8

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EUR 195

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EUR 234

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EUR 321.36

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EUR 375.96

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EUR 708.24

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EUR 195

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EUR 240.24

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SRM30-0025 25ml
EUR 723.84

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EUR 1071.72

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Bovine Trypsin Purified Diafiltered Sterile Filtered

IBOTRYPFSTFT250MG each
EUR 353
Description: Bovine Trypsin Purified Diafiltered Sterile Filtered

Bovine Trypsin Purified Diafiltered Sterile Filtered

IBOTRYPFSTFT50MG each
EUR 103
Description: Bovine Trypsin Purified Diafiltered Sterile Filtered

Sterile filtered Cynomolgus monkey serum, 0.2 micron

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EUR 1071.72

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EUR 517.92

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EUR 804.96

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EUR 2917.2

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EUR 8255.52

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H0910-010 100 ml
EUR 360

Sterile filtered rat serum, 0.2 micron, heat-inactivated

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EUR 312

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EUR 386.88

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EUR 659.88

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EUR 212.16

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EUR 246.48

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EUR 182.52

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EUR 213.72

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SM32-0050 50 ml
EUR 296.4

Sterile filtered mouse serum, 0.2 micron, heat-inactivated

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EUR 474.24

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EUR 609.96

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SM32-3000 3 L Ask for price

Sterile filtered sheep serum, 0.2 micron, heat-inactivated

SS32-0100 100 ml
EUR 221.52

Sterile filtered sheep serum, 0.2 micron, heat-inactivated

SS32-0500 500 ml
EUR 291.72

Sterile filtered feline serum, 0.2 micron, heat-inactivated

SF32-0100 100 ml
EUR 606.84

Sterile filtered feline serum, 0.2 micron, heat-inactivated

SF32-0500 500 ml
EUR 1410.24

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SC31-0050 50 ml
EUR 196.56

Sterile filtered chicken serum, 0.2 micron,charcoal stripped

SC31-0100 100 ml
EUR 223.08

Sterile filtered chicken serum, 0.2 micron, charcoal stripped

SC31-0500 500 ml
EUR 293.28

Sterile filtered equine serum, 0.2 micron, heat-inactivated

SE32-0100 100 ml
EUR 205.92

Sterile filtered equine serum, 0.2 micron, heat-inactivated

SE32-0500 500 ml
EUR 269.88

Sterile filtered donkey serum, 0.2 micron, heat-inactivated

SD32-0100 100 ml
EUR 205.92

Sterile filtered donkey serum, 0.2 micron, heat-inactivated

SD32-0500 500 ml
EUR 269.88

Sterile filtered canine serum, 0.2 micron, heat-inactivated

SCA32-0050 50 ml
EUR 296.4

Sterile filtered canine serum, 0.2 micron, heat-inactivated

SCA32-0100 100 ml
EUR 386.88

Sterile filtered canine serum, 0.2 micron, heat-inactivated

SCA32-0500 500 ml
EUR 730.08

Sterile filtered donkey serum, 0.2 micron, heat-inactivated

SD32-1000 1L
EUR 443.75

These results suggest that fetal fibroblast cell culture test pigs can be used as a valuable method for FBS screen for pig oocyte maturation and embryo development in vitro.

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