The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.

The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.

High -Mobilitas group box 1 (HMGB1) may act as chromatin structural proteins, and at the same time as the mediator of the immune system. a high correlation with graft acceptance on the receiver makes it a biomarker of pancreatic islet in islet transplantation.

With a pre-existing suspicion that HMGB1 in fetal bovine serum (FBS) will be detrimental to the survival and function of beta cells of murine, HMGB1 has been removed from the FBS and its effects investigated. Interestingly, HMGB1 abolition of FBS does not seem favorable for the survival and function of beta cultured murine cells, suggesting that HMGB1 that already exists in the FBS may be an integral component of islet cell culture.

Immunosuppressive Mechanisms of mesenchymal stem cells differently regulated by platelet poor plasma and bovine fetal serum supplemented media.

Mesenchymal Stem Cells (MSC) can interact with and modulate the immune function of all cells, pressing both the innate and adaptive immune responses. Currently, the majority of in vitro studies have led to the description of the property MSC has been generated from MSC culture in the presence of fetal bovine serum (FBS), in spite of the recognition of the limitations of FBS and an attempt to replace this component of the MSC media.

Herein, we compare FBS and platelets Poor Plasma (PPP) as a supplement MSC media, according to adipose-derived MSCs (AMSC) phenotype, proliferation and immunoregulatory mechanisms.Interestingly, although maintaining the classical phenotypic profile of MSC, cultured PPP AMSCs showed impaired proliferative potential.

Furthermore, our results clearly show that the culture of AMSC with PPP leads to decreased expression of soluble immunosuppressive factors, resulting in decreased capacity of inducing regulatory T-cells (Tregs) generation by these cells. Instead, the PPP supplements promoted increased VCAM-1 and ICAM-1 expression AMSC surface.

Therefore, AMSCs cultured with PPP showed limited potential to produce soluble immunomodulatory factors, showed a diminished capacity to control the immune system thought paracrine activity.Overall, our data warehouse stoked about the importance of supplementing the culture medium for MSC immunomodulatory behavior, as well as serve as a warning about complexity replace FBS in MSC culture.

The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.
The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.

Food-Derived Collagen Peptides, prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhancing the Growth of Primary Rat Skin Fibroblasts Using Cultivation of Fetal Bovine Serum Free from Hydroxyprolyl Peptide.

Prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly) appear in human blood after ingestion of hydrolyzed collagen and growth triggers fibroblasts embedded in the gel of collagen, which has been associated with a beneficial effect on the consumption of hydrolyzed collagen, such as skin repair and joint conditions.

In this study, the inconsistent results obtained using different lots of fetal bovine serum (FBS). Fibroblasts proliferated in collagen gel without adding a Pro-Hyp and the Hyp-Gly and did not respond to the addition of Pro-Hyp and the Hyp-Gly, which raises doubts about the conclusions of previous studies. Unexpectedly high levels of peptide hydroxyprolyl, including Pro-Hyp, however, present in the FBS (around 100 AD), and also in other forms commercially available from FBS (70-80 F). After the removal of low molecular weight (LMW, <6000 Da) compound from FBS by size exclusion chromatography, Pro-Hyp and the Hyp-Gly longer triggered fibroblast growth embedded in collagen and increase the number of fibroblasts migrate from the skin of mice.

Hyclone Characterized Fetal Bovine Serum US Origin 500 mL (SH30071.03)

HYC39 EACH
EUR 575.7

Hyclone Dialyzed Fetal Bovine Serum US Origin 50 mL (SH30079.01)

HYC43 EACH
EUR 92.34

Hyclone Dialyzed Fetal Bovine Serum US Origin 100 mL (SH30079.02)

HYC45 EACH
EUR 157.32

Hyclone Dialyzed Fetal Bovine Serum US Origin 500 mL (SH30079.03)

HYC47 EACH
EUR 656.64

Hyclone Standard Fetal Bovine Serum US Origin 100 mL (SH30088.02)

HYC56 EACH
EUR 112.86

Hyclone Standard Fetal Bovine Serum US Origin 500 mL (SH30088.03)

HYC58 EACH
EUR 471.96

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered US Origin

SLS1154 500ML
EUR 978.12

Hyclone Tetracycline Screened Fetal Bovine Serum US Origin 500 mL (SH30070.03T)

HYC32 EACH
EUR 1010.04

Hyclone Characterized Fetal Bovine Serum US Origin HeatInact 100 mL (SH30071.02HI)

HYC38 EACH
EUR 155.04

Hyclone Characterized Fetal Bovine Serum US Origin HeatInact 500 mL (SH30071.03HI)

HYC40 EACH
EUR 593.94

Hyclone Characterized Fetal Bovine Serum US Origin Irrad 500 mL (SH30071.03IR)

HYC42 EACH
EUR 600.78

Hyclone Dialyzed Fetal Bovine Serum US Origin Irradiated 500 mL (SH30079.03IR)

HYC50 EACH
EUR 694.26

FBS - Fetal Bovine Serum USDA Origin

FBS002 500 mL
EUR 574.8

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 500 mL (SH30068.03)

HYC11 EACH
EUR 656.64

Hyclone Defined Fetal Bovine Serum US Origin Heat Inactivated 50 mL (SH30070.01HI)

HYC16 EACH
EUR 135.66

Hyclone Defined Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30070.02HI)

HYC20 EACH
EUR 228

Hyclone Defined Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30070.03HI)

HYC27 EACH
EUR 865.26

Hyclone Insect Cell Screened Fetal Bovine Serum US Origin 500 mL (SH30070.03I)

HYC28 EACH
EUR 1010.04

Hyclone Characterized Fetal Bovine Serum US Origin Heat Inact 50 mL (SH30071.01HI)

HYC36 EACH
EUR 96.9

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 50 mL (SH30079.01HI)

HYC44 EACH
EUR 108.3

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30079.02HI)

HYC46 EACH
EUR 173.28

Hyclone Dialyzed Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30079.03HI)

HYC48 EACH
EUR 674.88

Hyclone Standard Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30088.02HI)

HYC57 EACH
EUR 128.82

Hyclone Standard Fetal Bovine Serum US Origin Heat Inact 500 mL (SH30088.03HI)

HYC59 EACH
EUR 489.06

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 50 mL (SH30068.01)

HYC7 EACH
EUR 92.34

Hyclone Super Low IgG Fetal Bovine Serum US Origin 100 mL (SH30898.02)

HYC72 EACH
EUR 171

Hyclone Super Low IgG Fetal Bovine Serum US Origin 500 mL (SH30898.03)

HYC75 EACH
EUR 711.36

Hyclone Charcoal/Dextran Treated Fetal Bovine Serum US Origin 100 mL (SH30068.02)

HYC9 EACH
EUR 157.32

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Heat Inactivated US Origin

SLS1156 500ML
EUR 957.6

500 ML FETAL BOVINE SERUM, AUSTRALIAN ORIGIN

35-076-CV 500 mL/pk
EUR 952.8
Description: Serum; Serum

Hyclone ES Screened Fetal Bovine Serum US Origin Heat Inact 100 mL (SH30070.02EH)

HYC19 EACH
EUR 257.64

Hyclone Super Low IgG Fetal Bovine Serum US Origin HeatInact 100 mL (SH30898.02HI)

HYC73 EACH
EUR 185.82

Hyclone Super Low IgG Fetal Bovine Serum US Origin HeatInact 500 mL (SH30898.03HI)

HYC76 EACH
EUR 728.46

Hyclone Super Low IgG Fetal Bovine Serum US Origin Irrad 500 mL (SH30898.03IR)

HYC78 EACH
EUR 728.46

Hyclone Bovine Growth Serum US Origin 100 mL (SH30541.02)

HYC67 EACH
EUR 43.32

Hyclone Bovine Growth Serum US Origin 500 mL (SH30541.03)

HYC69 EACH
EUR 188.1

Hyclone Bovine Growth Serum US Origin Irradiated 500 mL (SH30541.03IR)

HYC71 EACH
EUR 212.04

FBS - Fetal Bovine Serum

FBS001 500 ml
EUR 654

HQ Fetal Bovine Serum

abx098128-500ml 500 ml
EUR 895.2

EQ Fetal Bovine Serum

abx298025-500ml 500 ml
EUR 1362

Hyclone Bovine Growth Serum US Origin Heat Inactivated 100 mL (SH30541.02HI)

HYC68 EACH
EUR 59.28

Hyclone Bovine Growth Serum US Origin Heat Inactivated 500 mL (SH30541.03HI)

HYC70 EACH
EUR 206.34

FETAL BOVINE SERUM, REGULAR, NEW ZEALAND ORIGIN, GAMMA IRRADIATED, 500 ML

35-082-CV 500 mL/pk
EUR 1296
Description: Serum; Serum

Hyclone Research Grade Fetal Bovine Serum SouthAmerican Origin 100 mL (SV30160.02)

HYC83 EACH
EUR 57

Hyclone Research Grade Fetal Bovine Serum SouthAmerican Origin 500 mL (SV30160.03)

HYC85 EACH
EUR 241.68

Fetal Bovine Serum, Triple Low

F0610-050 500ml Ask for price

FBS Opti-Gold, US Origin

F0900-050 500ml
EUR 675.6

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1388.4

Fetal Bovine Serum, Hormone Level Reduced

F0620-050 500ml
EUR 902.4

Fetal Bovine Serum (FBS) [50 ml]

ABP-MED-FBS0050 50ml Ask for price

Fetal Bovine Serum (FBS) [500 ml]

ABP-MED-FBS0500 500ml Ask for price

GOOD fetal bovine serum - 500 mL

DDP40-37500 EACH
EUR 144.78

Rabbit Anti-Fetal Bovine Serum (FBS) protein IgG-Agarose affinity matrix to remove fetal bovine serum

FBS12-AS 2 ml
EUR 562.8

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested

CA068-010 100g
EUR 375.6

Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested

CA068-050 500g
EUR 1170

FBS-HI - Heat Inactivated Fetal Bovine Serum

FBS001-HI 500 ml
EUR 676.8

Fetal Bovine Serum (FBS) proteins for ELISA

FBS15-N-1 1 ml
EUR 270

500 ML FETAL BOVINE SERUM, PREMIUM (DIALYZED)

35-071-CV 500 mL/pk
EUR 1110
Description: Serum; Serum

Fetal Bovine Serum,Low IgG , < 5 ug/ml

F0710-050 500 ml
EUR 1437.6

Fetal Bovine Serum,IgG Depleted, < 2 ug/ml

F0720-050 500 ml
EUR 1437.6

Rabbit Anti-Fetal Bovine Serum (FBS) protein IgG

FBS11-A 100 ul
EUR 489.6

500 ML FETAL BOVINE SERUM, PREMIUM (GAMMA IRRADIATED)

35-070-CV 500 mL/pk
EUR 735.6
Description: Serum; Serum

500 ML FETAL BOVINE SERUM, PREMIUM (LOW IGG)

35-073-CV 500 mL/pk
EUR 2173.2
Description: Serum; Serum

500 ML FETAL BOVINE SERUM, PREMIUM (TETRACYCLINE NEGATIVE)

35-075-CV 500 mL/pk
EUR 1136.4
Description: Serum; Serum

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBA30-0050 50 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBA30-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBA30-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0050 50 ml
EUR 252.72

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0100 100 ml
EUR 283.92

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBM30-0500 500 ml
EUR 652.08

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBU30-0050 50 ml
EUR 285.48

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBU30-0100 100 ml
EUR 388.44

Sterile filtered fetal bovine serum, triple 0.1 micron filtered

SFBU30-0500 500 ml
EUR 751.92

BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, HEAT INACTIVATED

35-054-CM 1L/pk
EUR 327.6
Description: Serum; Serum

BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, GAMMA IRRADIATED

35-055-CM 1L/pk
EUR 387.6
Description: Serum; Serum

Fetal Bovien Serum, Charcol Stripped

F0700-010 100ml
EUR 481.2

Fetal Bovien Serum, Charcol Stripped

F0700-050 500ml
EUR 1064.4

CORNING® 500 ML FETAL BOVINE SERUM, PREMIUM (CHARCOAL STRIPPED)

35-072-CV 500 mL/pk
EUR 1171.2
Description: Serum; Serum

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

SFBMDY30-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

SFBMDY30-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

SFBUDY30-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered

SFBUDY30-0500 500 ml Ask for price

Chondroitin sulfate, from bovine origin

GC0982-1G 1 g
EUR 51.6

Chondroitin sulfate, from bovine origin

GC0982-25G 25 g
EUR 90

Chondroitin sulfate, from bovine origin

GC0982-50G 50 g
EUR 132

Chondroitin sulfate, from bovine origin

GC0982-5G 5 g
EUR 62.4

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBA32-0050 50 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBA32-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBA32-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered

SFBIU30-0050 50 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered

SFBIU30-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered

SFBIU30-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0100 100 ml
EUR 308.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0500 500 ml
EUR 780

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0050 50 ml
EUR 258.96

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0100 100 ml
EUR 291.72

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBM32-0500 500 ml
EUR 659.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBU31-0100 100 ml
EUR 354.12

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBU31-0500 500 ml
EUR 900.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0050 50 ml
EUR 293.28

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0100 100 ml
EUR 354.12

Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated

SFBU32-0500 500 ml
EUR 759.72

500ML LSG Fetal Bovine Serum (FBS) -Sterile Filtered - Heat Inactivated 500ml

SLS1150 500ML
EUR 296.4

500ML LSG Fetal Bovine Serum (FBS) - Sterile Filtered - Gamma Irradiated 500ml

SLS1152 500ML
EUR 444.6

BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, HEAT INACTIVATED, GAMMA IRRADIATED

35-056-CM 1L/pk
EUR 447.6
Description: Serum; Serum

Purified fetal calf/bovine Hemoglobin

HEMG18-N-1 1 mg Ask for price

Goat Complement serum, (Lyophilized), tested for complement activity

COMPGT-1 1 ml
EUR 270

Llama Complement serum, (Lyophilized), tested for complement activity

COMPLA-1 1 ml
EUR 343.2

Mouse Complement serum, (Lyophilized), tested for complement activity

COMPMO-1 1 ml
EUR 343.2

Rat Complement serum, (Lyophilized), tested for complement activity

COMPRT-1 1 ml
EUR 343.2

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated

SFBMDY32-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated

SFBMDY32-0500 500 ml Ask for price

Hyclone Tetracycline Screened FBS US Origin Heat Inactivated 500 mL (SH30070.03TH)

HYC33 EACH
EUR 1026

Hyclone Characterized FBS US Origin Irradiated Heat Inactiv 500 mL (SH30071.03IH)

HYC41 EACH
EUR 611.04

Bovine Serum Albumin

7-04585 10gr Ask for price

Bovine Serum Albumin

7-04586 50gr Ask for price

Bovine Serum Albumin

7-04587 100gr Ask for price

Normal Bovine Serum

abx098263-100ml 100 ml
EUR 309.6

Normal Bovine Serum

88R-B001 100 ml
EUR 198
Description: Control Normal Bovine Serum

Bovine Serum Albumin

HY-D0842 10g
EUR 224.4

Bovine Serum Albumin

GE1877-100G 100 g
EUR 189.6

Bovine Serum Albumin

GE1877-10G 10 g
EUR 64.8

Bovine Serum Albumin

GE1877-250G 250 g
EUR 342

Bovine Serum Albumin

GE1877-25G 25 g
EUR 84

Bovine Serum Albumin

GE1877-500G 500 g
EUR 600

Bovine Serum Albumin

GE1877-50G 50 g
EUR 122.4

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

SFBIU32-0050 50 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

SFBIU32-0100 100 ml Ask for price

Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated

SFBIU32-0500 500 ml Ask for price

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0100 100 ml
EUR 330.72

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0500 500 ml
EUR 787.8

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0100 100 ml
EUR 360.36

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0500 500 ml
EUR 907.92

Baby Rabbit Complement serum (Lyophilized), tested for complement activity

COMPBR-10 10 ml
EUR 634.8

Baby Rabbit Complement serum (Lyophilized), tested for complement activity

COMPBR-2 2 ml
EUR 270

Standard Rabbit Complement serum (Lyophilized), tested for complement activity

COMPR-2 2 ml
EUR 343.2

Standard Rabbit Complement serum (Lyophilized), tested for complement activity

COMPR-20 20 ml
EUR 489.6

Standard Rabbit Complement serum (Lyophilized), tested for complement activity

COMPR-5 5 ml
EUR 343.2

Hyclone Charcoal/Dextran Treated FBS US Origin Heat Inactiv 100 mL (SH30068.02HI)

HYC10 EACH
EUR 173.28

Hyclone Char/Dext Treated FBS US Origin Heat Inactivated 500 mL (SH30068.03HI)

HYC12 EACH
EUR 674.88

Hyclone Human Mesenchymal Stem Cell Screened FBS US Origin 500 mL (SH30070.03M)

HYC31 EACH
EUR 1010.04

Hyclone Dialyzed FBS US Origin Irradiated and Heat Inactiv 500 mL (SH30079.03IH)

HYC49 EACH
EUR 690.84

Hyclone Standard FBS US Origin Irradiated and Heat Inactiv 500 mL (SH30088.03IH)

HYC60 EACH
EUR 506.16

Hyclone Charcoal/Dextran Treated FBS US Origin Heat Inactiv 50 mL (SH30068.01HI)

HYC8 EACH
EUR 108.3

Immortalized Fetal Bovine Kidney Cells - SV40

T0081 1x106 cells / 1.0 ml Ask for price

Immortalized Fetal Bovine Lung Cells - SV40

T0091 1x106 cells / 1.0 ml Ask for price

These results indicate the presence of bioactive peptides hydroxyprolyl in FBS commercially available, which can mask the effects of Pro-Hyp and Hyp-Gly supplements; Our work confirms that Pro-Hyp and the Hyp-Gly do not play an important role in the proliferation of fibroblasts.

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