High -Mobilitas group box 1 (HMGB1) may act as chromatin structural proteins, and at the same time as the mediator of the immune system. a high correlation with graft acceptance on the receiver makes it a biomarker of pancreatic islet in islet transplantation.
With a pre-existing suspicion that HMGB1 in fetal bovine serum (FBS) will be detrimental to the survival and function of beta cells of murine, HMGB1 has been removed from the FBS and its effects investigated. Interestingly, HMGB1 abolition of FBS does not seem favorable for the survival and function of beta cultured murine cells, suggesting that HMGB1 that already exists in the FBS may be an integral component of islet cell culture.
Immunosuppressive Mechanisms of mesenchymal stem cells differently regulated by platelet poor plasma and bovine fetal serum supplemented media.
Mesenchymal Stem Cells (MSC) can interact with and modulate the immune function of all cells, pressing both the innate and adaptive immune responses. Currently, the majority of in vitro studies have led to the description of the property MSC has been generated from MSC culture in the presence of fetal bovine serum (FBS), in spite of the recognition of the limitations of FBS and an attempt to replace this component of the MSC media.
Herein, we compare FBS and platelets Poor Plasma (PPP) as a supplement MSC media, according to adipose-derived MSCs (AMSC) phenotype, proliferation and immunoregulatory mechanisms.Interestingly, although maintaining the classical phenotypic profile of MSC, cultured PPP AMSCs showed impaired proliferative potential.
Furthermore, our results clearly show that the culture of AMSC with PPP leads to decreased expression of soluble immunosuppressive factors, resulting in decreased capacity of inducing regulatory T-cells (Tregs) generation by these cells. Instead, the PPP supplements promoted increased VCAM-1 and ICAM-1 expression AMSC surface.
Therefore, AMSCs cultured with PPP showed limited potential to produce soluble immunomodulatory factors, showed a diminished capacity to control the immune system thought paracrine activity.Overall, our data warehouse stoked about the importance of supplementing the culture medium for MSC immunomodulatory behavior, as well as serve as a warning about complexity replace FBS in MSC culture.
The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.
Food-Derived Collagen Peptides, prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhancing the Growth of Primary Rat Skin Fibroblasts Using Cultivation of Fetal Bovine Serum Free from Hydroxyprolyl Peptide.
Prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly) appear in human blood after ingestion of hydrolyzed collagen and growth triggers fibroblasts embedded in the gel of collagen, which has been associated with a beneficial effect on the consumption of hydrolyzed collagen, such as skin repair and joint conditions.
In this study, the inconsistent results obtained using different lots of fetal bovine serum (FBS). Fibroblasts proliferated in collagen gel without adding a Pro-Hyp and the Hyp-Gly and did not respond to the addition of Pro-Hyp and the Hyp-Gly, which raises doubts about the conclusions of previous studies. Unexpectedly high levels of peptide hydroxyprolyl, including Pro-Hyp, however, present in the FBS (around 100 AD), and also in other forms commercially available from FBS (70-80 F). After the removal of low molecular weight (LMW, <6000 Da) compound from FBS by size exclusion chromatography, Pro-Hyp and the Hyp-Gly longer triggered fibroblast growth embedded in collagen and increase the number of fibroblasts migrate from the skin of mice.
Fetal Bovine Serum, Premium, US Origin , Heat Inactivated
F0601-050
GenDepot
500ml
EUR 837.6
FBS - Fetal Bovine Serum USDA Origin
FBS002
Neuromics
500 mL
EUR 574.8
500 ML FETAL BOVINE SERUM, AUSTRALIAN ORIGIN
35-076-CV
CORNING
500 mL/pk
EUR 952.8
Description: Serum; Serum
FETAL BOVINE SERUM, REGULAR, NEW ZEALAND ORIGIN, GAMMA IRRADIATED, 500 ML
35-082-CV
CORNING
500 mL/pk
EUR 1296
Description: Serum; Serum
HQ Fetal Bovine Serum
abx098128-500ml
Abbexa
500 ml
EUR 895.2
EQ Fetal Bovine Serum
abx298025-500ml
Abbexa
500 ml
EUR 1362
FBS - Fetal Bovine Serum
FBS001
Neuromics
500 ml
EUR 654
Fetal Bovine Serum, Triple Low
F0610-050
GenDepot
500ml
Ask for price
Fetal Bovine Serum(Best-Quality )
FBSAD-01011
Cyagen
100/500mL
Ask for price
Fetal Bovine Serum (FBS) [50 ml]
ABP-MED-FBS0050
Allele Biotech
50ml
Ask for price
Fetal Bovine Serum (FBS) [500 ml]
ABP-MED-FBS0500
Allele Biotech
500ml
Ask for price
Fetal Bovine Serum(Without Exosome )
FBSNE-01061
Cyagen
20/50mL
Ask for price
Fetal Bovine Serum(Superior-Quality)
FBSSR-01021
Cyagen
500mL
EUR 939
Fetal Bovine Serum(Standard-Quality)
FBSST-01033
Cyagen
500mL
EUR 599
Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested
CA068-010
GenDepot
100g
EUR 375.6
Bovine Serum Albumin, Low Endotoxin, Cell Cultre Tested
CA068-050
GenDepot
500g
EUR 1170
Fetal Bovine Serum, Hormone Level Reduced
F0620-050
GenDepot
500ml
EUR 902.4
Fetal Bovine Serum, Premium Tetracyclin Free
F0500-050
GenDepot
500ml
EUR 1388.4
Fetal Bovine Serum,Low IgG , < 5 ug/ml
F0710-050
GenDepot
500 ml
EUR 1437.6
500 ML FETAL BOVINE SERUM, PREMIUM (DIALYZED)
35-071-CV
CORNING
500 mL/pk
EUR 1110
Description: Serum; Serum
500 ML FETAL BOVINE SERUM, PREMIUM (LOW IGG)
35-073-CV
CORNING
500 mL/pk
EUR 2173.2
Description: Serum; Serum
FBS-HI - Heat Inactivated Fetal Bovine Serum
FBS001-HI
Neuromics
500 ml
EUR 676.8
Fetal Bovine Serum,IgG Depleted, < 2 ug/ml
F0720-050
GenDepot
500 ml
EUR 1437.6
Rabbit Anti-Fetal Bovine Serum (FBS) protein IgG-Agarose affinity matrix to remove fetal bovine serum
FBS12-AS
Alpha Diagnostics
2 ml
EUR 562.8
500 ML FETAL BOVINE SERUM, PREMIUM (GAMMA IRRADIATED)
35-070-CV
CORNING
500 mL/pk
EUR 735.6
Description: Serum; Serum
500 ML FETAL BOVINE SERUM, PREMIUM (TETRACYCLINE NEGATIVE)
35-075-CV
CORNING
500 mL/pk
EUR 1136.4
Description: Serum; Serum
BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, HEAT INACTIVATED
35-054-CM
CORNING
1L/pk
EUR 327.6
Description: Serum; Serum
BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, GAMMA IRRADIATED
35-055-CM
CORNING
1L/pk
EUR 387.6
Description: Serum; Serum
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBA30-0050
Equitech
50 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBA30-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBA30-0500
Equitech
500 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBM30-0050
Equitech
50 ml
EUR 252.72
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBM30-0100
Equitech
100 ml
EUR 283.92
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBM30-0500
Equitech
500 ml
EUR 652.08
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0050
Equitech
50 ml
EUR 285.48
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0100
Equitech
100 ml
EUR 388.44
Sterile filtered fetal bovine serum, triple 0.1 micron filtered
SFBU30-0500
Equitech
500 ml
EUR 751.92
CORNING® 500 ML FETAL BOVINE SERUM, PREMIUM (CHARCOAL STRIPPED)
35-072-CV
CORNING
500 mL/pk
EUR 1171.2
Description: Serum; Serum
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBMDY30-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBMDY30-0500
Equitech
500 ml
Ask for price
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBUDY30-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered
SFBUDY30-0500
Equitech
500 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0050
Equitech
50 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered
SFBIU30-0500
Equitech
500 ml
Ask for price
BOVINE CALF SERUM, UNITED STATES ORIGIN, IRON FORTIFIED, HEAT INACTIVATED, GAMMA IRRADIATED
35-056-CM
CORNING
1L/pk
EUR 447.6
Description: Serum; Serum
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0100
Equitech
100 ml
EUR 308.88
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0500
Equitech
500 ml
EUR 780
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0100
Equitech
100 ml
EUR 354.12
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0500
Equitech
500 ml
EUR 900.12
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0050
Equitech
50 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBA32-0500
Equitech
500 ml
Ask for price
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0050
Equitech
50 ml
EUR 258.96
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0100
Equitech
100 ml
EUR 291.72
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBM32-0500
Equitech
500 ml
EUR 659.88
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0050
Equitech
50 ml
EUR 293.28
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0100
Equitech
100 ml
EUR 354.12
Sterile filtered fetal bovine serum, triple 0.1 micron filtered, heat-inactivated
SFBU32-0500
Equitech
500 ml
EUR 759.72
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated
SFBMDY32-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, heat-inactivated
SFBMDY32-0500
Equitech
500 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0050
Equitech
50 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0100
Equitech
100 ml
Ask for price
Sterile filtered fetal bovine serum, low IgG, triple 0.1 micron filtered, heat-inactivated
SFBIU32-0500
Equitech
500 ml
Ask for price
Fetal Bovien Serum, Charcol Stripped
F0700-010
GenDepot
100ml
EUR 481.2
Fetal Bovien Serum, Charcol Stripped
F0700-050
GenDepot
500ml
EUR 1064.4
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0100
Equitech
100 ml
EUR 330.72
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0500
Equitech
500 ml
EUR 787.8
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0100
Equitech
100 ml
EUR 360.36
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0500
Equitech
500 ml
EUR 907.92
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC941270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF594 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC941270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF594 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC801270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF680 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC801270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF680 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC811270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF680R conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC811270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF680R conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC701270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF770 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC701270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF770 conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC881270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF488A conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC881270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF488A conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC051270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF405M conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC051270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF405M conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC041270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF405S conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC041270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF405S conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC401270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF640R conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNC401270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), CF640R conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCB1270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Biotin conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCB1270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Biotin conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCAP1270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCAP1270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCA1270-250
Biotium
250uL
EUR 459.6
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), APC conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCR1270-250
Biotium
250uL
EUR 459.6
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), RPE conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCP1270-250
Biotium
250uL
EUR 459.6
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), PerCP conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNUM1270-50
Biotium
50uL
EUR 474
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), 1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCH1270-100
Biotium
100uL
EUR 238.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) (IL6/1270) Antibody
BNCH1270-500
Biotium
500uL
EUR 652.8
Description: Primary antibody against IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) ( IL6/1270), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL
These results indicate the presence of bioactive peptides hydroxyprolyl in FBS commercially available, which can mask the effects of Pro-Hyp and Hyp-Gly supplements; Our work confirms that Pro-Hyp and the Hyp-Gly do not play an important role in the proliferation of fibroblasts.